Modelling and measurements of fibrinogen adsorption on positively charged microspheres

• Дата: 2016
• Автори: Zeliszewska, P., Bratek-Skicki, A., Adamczyk, Z., Ciesla, M.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут фізики конденсованих систем НАН України 2016
• Назва видання: Condensed Matter Physics
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/155785
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ.

Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maximum coverage of fibrinogen on latex particles was precisely determined by the AFM method. Unexpectedly, at pH = 3.5, where both fibrinogen molecule and the latex particles were positively charged, the maximum coverage varied between 0.9 mg m⁻² and 1.1 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. On the other hand, at pH = 9.7, the maximum coverage of fibrinogen was larger, varying between 1.8 mg m⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations.