Modelling and measurements of fibrinogen adsorption on positively charged microspheres
Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maxim...
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| Дата: | 2016 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут фізики конденсованих систем НАН України
2016
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| Назва видання: | Condensed Matter Physics |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/155785 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ. |
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irk-123456789-1557852019-06-18T01:28:34Z Modelling and measurements of fibrinogen adsorption on positively charged microspheres Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maximum coverage of fibrinogen on latex particles was precisely determined by the AFM method. Unexpectedly, at pH = 3.5, where both fibrinogen molecule and the latex particles were positively charged, the maximum coverage varied between 0.9 mg m⁻² and 1.1 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. On the other hand, at pH = 9.7, the maximum coverage of fibrinogen was larger, varying between 1.8 mg m⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations. Теоретично та експериментально дослiджено адсорбцiю фiбриногену на позитивно заряджених мiкросферах. Структура моношарiв i максимальне покриття були визначенi експериментально при pH = 3.5 i 9.7 для концентрацiї NaCl в дiапазонi 10⁻³ − 0.15 M. Максимальне покриття фiбриногену на частинках латексу було визначене прецизiйно методом мiкроскопу атомної сили (AFM). Несподiвано, що при pH = 3.5, коли i фiбриноген i частинки латексу зарядженi позитивно, максимальне покриття змiнюється мiж 0.9 мг м⁻² i 1.1 мг м⁻² для 10⁻² i 0.15 M NaCl, вiдповiдно. При pH = 9.7 максимальне покриття фiбриногену є бiльшим i змiнюється вiд 1.8 мг м⁻² до 3.4 мг м⁻² для 10⁻² i 0.15 M NaCl, вiдповiдно. Експериментальнi результати пiдтвердженi чисельним моделюванням. 2016 Article Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ. 1607-324X DOI:10.5488/CMP.19.13801 arXiv:1603.02437 PACS: 82.70.Dd, 87.15A, 87.14.E http://dspace.nbuv.gov.ua/handle/123456789/155785 en Condensed Matter Physics Інститут фізики конденсованих систем НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| description |
Adsorption of fibrinogen on positively charged microspheres was theoretically and experimentally studied. The structure of monolayers and the maximum coverage were determined by applying the experimental measurements at pH = 3.5 and 9.7 for NaCl concentration in the range of 10⁻³ - 0.15 M. The maximum coverage of fibrinogen on latex particles was precisely determined by the AFM method. Unexpectedly, at pH = 3.5, where both fibrinogen molecule and the latex particles were positively charged, the maximum coverage varied between 0.9 mg m⁻² and 1.1 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. On the other hand, at pH = 9.7, the maximum coverage of fibrinogen was larger, varying between 1.8 mg m⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations⁻² and 3.4 mg m⁻² for 10⁻² and 0.15 M NaCl, respectively. The experimental results were quantitatively interpreted by the numerical simulations. |
| format |
Article |
| author |
Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. |
| spellingShingle |
Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. Modelling and measurements of fibrinogen adsorption on positively charged microspheres Condensed Matter Physics |
| author_facet |
Zeliszewska, P. Bratek-Skicki, A. Adamczyk, Z. Ciesla, M. |
| author_sort |
Zeliszewska, P. |
| title |
Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_short |
Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_full |
Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_fullStr |
Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_full_unstemmed |
Modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| title_sort |
modelling and measurements of fibrinogen adsorption on positively charged microspheres |
| publisher |
Інститут фізики конденсованих систем НАН України |
| publishDate |
2016 |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/155785 |
| citation_txt |
Modelling and measurements of fibrinogen adsorption on positively charged microspheres / P. Zeliszewska, A. Bratek-Skicki, Z. Adamczyk, M. Ciesla // Condensed Matter Physics. — 2016. — Т. 19, № 1. — С. 13801: 1–14. — Бібліогр.: 42 назв. — англ. |
| series |
Condensed Matter Physics |
| work_keys_str_mv |
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2023-05-20T17:48:04Z |
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2023-05-20T17:48:04Z |
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