Disturbances in lipid second messengers generation by stimulated blood lymphocytes in breast cancer
Aim. The main objective of thisstudy wasthe comparative investigation of diverse lipid second messenger(LSM) generation by human peripheral blood lymphocytes (HPBL) at different (5, 10, 30 and 60 s) time points of cell co-stimulation by anti-CD3 and anti-CD28 monoclonal antibodies in norm and brea...
Збережено в:
| Дата: | 2011 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
2011
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| Назва видання: | Вiopolymers and Cell |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/156351 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Disturbances in lipid second messengers generation by stimulated blood lymphocytes in breast cancer / M.P. Lazyan, H.H. Davtyan, T.B. Batikyan, R.A. Kazaryan, K.A. Alexanyan, H.M. Galstyan, Yu.V. Tadevosyan // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 387-389. — Бібліогр.: 4 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Резюме: | Aim. The main objective of thisstudy wasthe comparative investigation of diverse lipid second messenger(LSM)
generation by human peripheral blood lymphocytes (HPBL) at different (5, 10, 30 and 60 s) time points of cell
co-stimulation by anti-CD3 and anti-CD28 monoclonal antibodies in norm and breast cancer (BC). Methods.
Ficoll-Hypaque gradient centrifugation. Results. The data obtained indicate that some mechanisms of LSM generation/utilization in stimulated crude HPBL were significantly altered in BC compared to norm. Particularly,
the reliable generation of arachidonyl-1,2-diacylglycerol (1,2-DAG) at the initial step (5 s) of cell stimulation
observed in norm was depressed in BC and reached the value below the basal level in unstimulated cells. It is
important that the disturbances in 1,2-DAG formation in HPBL obtained from patients with BC were identical
with those observed earlier in other forms of cancer. Conclusions. We conclude that the regularities revealed
are common characteristics for all the types of malignancy studied and can be used as additional testing parameters for cancer definition and individual correction of the chemotherapy programs for disease treatment.
Keywords: blood lymphocytes, lipid second messengers, breast cancer. |
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