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Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior

Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior

Aim. To study subcellular nitrergic response in the rat brain regions following chronic stress-induced depression-like behavior. Methods. An animal model of depression induced by chronic circadian stress (CCS) established in our laboratory was used. The L-arginine, L-citrulline and reactive nitrogen...

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Main Authors: Nazaryan, N.S., Movsesyan, N.H., Alchujyan, N.Kh., Movsesyan, H.A., Guevorkian, A.G., Hairapetyan, H.L., Barsegyan, K.A., Kevorkian, G.A.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2011
Series:Вiopolymers and Cell
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/156357
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description Aim. To study subcellular nitrergic response in the rat brain regions following chronic stress-induced depression-like behavior. Methods. An animal model of depression induced by chronic circadian stress (CCS) established in our laboratory was used. The L-arginine, L-citrulline and reactive nitrogen species (RNS) levels were determined spectrophotometrically. Results. Immediately after CCS and four dayslater, а depression-like behavior of rats was observed and accompanied by a substantial persistent elevation of the L-arginine, L-citrulline and RNS levels with a simultaneous up-regulation of the inducible nitric oxide synthase (iNOS) in both cytosolic and mitochondrial compartments of the rat prefrontal cortex, striatum, hippocampus, and hypothalamus, and a down-regulation of their cytosolic constitutive NOS isoforms (cNOS), mitochondrial cNOS was not significantly changed, with the exception for hypothalamus, in which the latter dropped. Conclusions. Compromised balance of the L-arginine levels and NO synthesisin both mitochondria and cytosol in the limbic brain appearsto be implicated in the pathogenesis of depression and pathological anxiety. Keywords: L-arginine, brain, chronic stress, depression-like behavior, mitochondria, nitric oxide synthase.
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author Nazaryan, N.S.
Movsesyan, N.H.
Alchujyan, N.Kh.
Movsesyan, H.A.
Guevorkian, A.G.
Hairapetyan, H.L.
Barsegyan, K.A.
Kevorkian, G.A.
spellingShingle Nazaryan, N.S.
Movsesyan, N.H.
Alchujyan, N.Kh.
Movsesyan, H.A.
Guevorkian, A.G.
Hairapetyan, H.L.
Barsegyan, K.A.
Kevorkian, G.A.
Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
Вiopolymers and Cell
author_facet Nazaryan, N.S.
Movsesyan, N.H.
Alchujyan, N.Kh.
Movsesyan, H.A.
Guevorkian, A.G.
Hairapetyan, H.L.
Barsegyan, K.A.
Kevorkian, G.A.
author_sort Nazaryan, N.S.
title Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
title_short Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
title_full Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
title_fullStr Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
title_full_unstemmed Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
title_sort region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior
publisher Інститут молекулярної біології і генетики НАН України
publishDate 2011
url http://dspace.nbuv.gov.ua/handle/123456789/156357
citation_txt Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior / N.S. Nazaryan, N.H. Movsesyan, N.Kh. Alchujyan, H.A. Movsesyan, A.G. Guevorkian, H.L. Hairapetyan, K.A. Barsegyan, G.A. Kevorkian // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 394-397. — Бібліогр.: 12 назв. — англ.
series Вiopolymers and Cell
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last_indexed 2023-05-20T17:43:22Z
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spelling irk-123456789-1563572019-06-19T01:24:41Z Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior Nazaryan, N.S. Movsesyan, N.H. Alchujyan, N.Kh. Movsesyan, H.A. Guevorkian, A.G. Hairapetyan, H.L. Barsegyan, K.A. Kevorkian, G.A. Aim. To study subcellular nitrergic response in the rat brain regions following chronic stress-induced depression-like behavior. Methods. An animal model of depression induced by chronic circadian stress (CCS) established in our laboratory was used. The L-arginine, L-citrulline and reactive nitrogen species (RNS) levels were determined spectrophotometrically. Results. Immediately after CCS and four dayslater, а depression-like behavior of rats was observed and accompanied by a substantial persistent elevation of the L-arginine, L-citrulline and RNS levels with a simultaneous up-regulation of the inducible nitric oxide synthase (iNOS) in both cytosolic and mitochondrial compartments of the rat prefrontal cortex, striatum, hippocampus, and hypothalamus, and a down-regulation of their cytosolic constitutive NOS isoforms (cNOS), mitochondrial cNOS was not significantly changed, with the exception for hypothalamus, in which the latter dropped. Conclusions. Compromised balance of the L-arginine levels and NO synthesisin both mitochondria and cytosol in the limbic brain appearsto be implicated in the pathogenesis of depression and pathological anxiety. Keywords: L-arginine, brain, chronic stress, depression-like behavior, mitochondria, nitric oxide synthase. Мета. Вивчення субклітинної нітрергічної відповіді у ділянках мозку щурів при депресивноподібній поведінці, індукованій хронічним стресом. Методи. Використано розроблену в нашій лабораторії тваринну (щур) модель депресії, індуковану хронічним циркадіанним стресом (ХЦС). L-аргінін, L-цитрулін і активні форми азоту (АФА) визначали спектрофотометрично. Результати. Відразу після ХЦС та чотири дні потому спостерігається депресивноподібна поведінка щурів, яка супроводжується значним підвищенням вмісту L-аргініну, L-цитруліну та АФА з одночасним стимулюванням індуцибельної ізоформи NO синтази в мітохондріях та цитозолі префронтального кортексу, стріатуму, гіпокампа і гіпоталамуса. При цьому відмічено пригнічення загальної активності конститутивних ізоформ NOS (cNOS) у цитозолі цих відділів мозку, тоді як для мітохондріальної cNOS статистично вірогідних змін не виявлено, за винятком зменшення її активності у гіпоталамусі. Висновки. Порушення вмісту L-аргініну і збалансованого синтезу NO в лімбічному мозку, очевидно, причетні до патогенезу депресії і тривоги. Ключові слова: L-аргінін, мозок, хронічний стрес, депресивноподібна поведінка, мітохондрії, синтаза оксиду азоту. Цель. Изучение субклеточного нитрергического ответа в участках мозга крыс при депрессивноподобном поведении, индуцированном хроническим стрессом. Методы. Использовали разработанную в нашей лаборатории животную (крыса) модель депрессии, индуцированную хроническим циркадианным стрессом (ХЦС). L-аргинин, L-цитруллин и активные формы азота (АФА) определяли спектрофотометрически. Результаты. Сразу после ХЦС и четыре дня спустя наблюдается депрессивноподобное поведение крыс, сопровождающееся значительным повышением содержания L-аргинина, L-цитруллина и АФА, с одновременным стимулированием индуцибельной изоформы NO синтазы в митохондриях и цитозоле префронтального кортекса, стриатума, гиппокампа и гипоталамуса. При этом отмечено подавление общей активности конститутивных изоформ NOS (cNOS) в цитозоле этих отделов мозга, тогда как для митохондриальной cNOS статистически достоверных изменений не выявлено, за исключением уменьшения ее активности в гипоталамусе. Выводы. Нарушения в содержании L-аргинина и сбалансированного синтеза NO в лимбическом мозге, повидимому, причастны к патогенезу депрессии и тревоги. Ключевые слова: L-аргинин, мозг, хронический стресс, депрессивноподобное поведение, митохондрии, синтаза оксида азота. 2011 Article Region-specific nitric oxide production in cytosolic and mitochondrial compartments of the rat brain tissues following chronic stress-induced depression-like behavior / N.S. Nazaryan, N.H. Movsesyan, N.Kh. Alchujyan, H.A. Movsesyan, A.G. Guevorkian, H.L. Hairapetyan, K.A. Barsegyan, G.A. Kevorkian // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 394-397. — Бібліогр.: 12 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.00012C http://dspace.nbuv.gov.ua/handle/123456789/156357 577.158:616.831-005 en Вiopolymers and Cell Інститут молекулярної біології і генетики НАН України

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