Selective cytotoxicity and modification activity of picornaviruses on transformed cell lines

Aim. We do analyze of the dynamics of morphometabolic changes in transformed cells (of susceptoible lines) demonstrating resistance to picrnaviral infection. Methods. The study was performed by application of cell culture technology and a complex of cytochemical and cytophotometric assays. Were us...

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Бібліографічні деталі
Дата:2011
Автори: Karalyan, Z.A., Avagyan, H.R.
Формат: Стаття
Мова:English
Опубліковано: Інститут молекулярної біології і генетики НАН України 2011
Назва видання:Вiopolymers and Cell
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/156377
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Selective cytotoxicity and modification activity of picornaviruses on transformed cell lines / Z.A. Karalyan, H.R. Avagyan // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 377-380. — Бібліогр.: 8 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
Опис
Резюме:Aim. We do analyze of the dynamics of morphometabolic changes in transformed cells (of susceptoible lines) demonstrating resistance to picrnaviral infection. Methods. The study was performed by application of cell culture technology and a complex of cytochemical and cytophotometric assays. Were used picornaviruses from various genu. Results. According to the results obtained, resistant to picornavirus infection cells of different susceptible lines have similar changes in the phenotype. They have decreased number of nucleoli and increased percentage of euploidy (and near euploid). In resistant cells of all cultures the reduction in amount of DNA and RNA both in nucleus and in cytoplasm wasfound. All these data correlated with the increased euploidy (and near euploid) of the resistant population. All picornavirus resistant cells had a less transformed phenotype, and decreased proliferative activity. Decreased nucleolar status becomes apparent by reduction of all nucleolar indices. Conclusions. Picornaviruses on the susceptible cells produce 2 types of changes – selection and modification. Whateverthe mechanism, it isspecific for an individual virus, since no restrictions occur in case of infection caused by another picornavirus. Keywords: picornavirus, euploidy, nucleus, nucleolus.