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Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells

Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells

Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Metho...

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Main Authors: Gubar, O.S., Kropyvko, S.V., Tsyba, L.O., Gasman, S., Rynditch, A.V.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2012
Series:Вiopolymers and Cell
Subjects:
Structure and Function of Biopolymers
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/156729
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spelling irk-123456789-1567292019-06-19T01:30:03Z Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells Gubar, O.S. Kropyvko, S.V. Tsyba, L.O. Gasman, S. Rynditch, A.V. Structure and Function of Biopolymers Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Methods. During this study we employed immunoprecipitation and confocal microscopy. Results. We have shown that endogenous ITSN1-S coprecipitates with overexpressed ITSN1-L in PC12, 293 and 293T cells. Long and short isoforms of ITSN1 also colocalize in 293T cells. Conclusions. ITSN1-L and ITSN1-S form complexes in mammalian cells. Keywords: ITSN1, alternatively spliced isoforms, adaptor/scaffold proteins. Інтерсетин 1 (ITSN1) – адатоний білок, залучений до мембранного транспорту та передачі клітинних сигналів. Довга і коротка ізоформи ITSN1 (ITSN1-L і ITSN1-S) утворюються в результаті альтернативного сплайсингу. Метою роботи було встановити, чи можуть ITSN1-L і ITSN1-S взаємодіяти в клітинах ссавців. Методи. Використано методи імунопреципітації та конфокальної мікроскопії. Результати. Показано, що ендогенний ITSN1- S копреципітується з надекспресованим ITSN1-L у клітинах PC12, 293 і 293T. Довга і коротка ізоформи ITSN1 також колокалізуються у клітинах 293T. Висновки. ITSN1-L і ITSN1-S формують комплекси в клітинах ссавців. Ключові слова: ITSN1, альтернативно сплайсовані ізоформи, адапторні білки. Интерсектин 1 (ITSN1) – адапторный белок, участвующий в мембранном транспорте и передаче клеточных сигналов. Длинная и короткая изоформы ITSN1 (ITSN1-L и ITSN1-S) образуются вследствие альтернативного сплайсинга. Целью исследования было выяснить, могут ли ITSN1-L и ITSN1-S взаимодействовать в клетках млекопитающих. Методы. Использованы методы иммунопреципитации и конфокальной микроскопии. Результаты. Показано, что эндогенный ITSN1-S копреципитируется со сверхэкпрессированным ITSN1-L в клетках PC12, 293 и 293T. Длинная и короткая изоформы ITSN1 так же колокализуются в клетках 293T. Выводы. ITSN1-L и ITSN1-S формируют комплексы в клетках млекопитающих. Ключевые слова: ITSN1, альтернативно сплайсированные изоформы, адапторные белки. 2012 Article Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells / O.S. Gubar, S.V. Kropyvko, L.O. Tsyba, S. Gasman, A.V. Rynditch // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 429-423. — Бібліогр.: 29 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.000132 http://dspace.nbuv.gov.ua/handle/123456789/156729 577.22 en Вiopolymers and Cell Інститут молекулярної біології і генетики НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Structure and Function of Biopolymers
Structure and Function of Biopolymers
spellingShingle Structure and Function of Biopolymers
Structure and Function of Biopolymers
Gubar, O.S.
Kropyvko, S.V.
Tsyba, L.O.
Gasman, S.
Rynditch, A.V.
Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
Вiopolymers and Cell
description Intersectin 1 (ITSN1) is an adaptor protein involved in membrane trafficking and cell signaling. Long and short isoforms of ITSN1 (ITSN1-L and ITSN1-S) are produced by alternative splicing. The aim of our study was to investigate whether ITSN1-L and ITSN1-S could interact in mammalian cells. Methods. During this study we employed immunoprecipitation and confocal microscopy. Results. We have shown that endogenous ITSN1-S coprecipitates with overexpressed ITSN1-L in PC12, 293 and 293T cells. Long and short isoforms of ITSN1 also colocalize in 293T cells. Conclusions. ITSN1-L and ITSN1-S form complexes in mammalian cells. Keywords: ITSN1, alternatively spliced isoforms, adaptor/scaffold proteins.
format Article
author Gubar, O.S.
Kropyvko, S.V.
Tsyba, L.O.
Gasman, S.
Rynditch, A.V.
author_facet Gubar, O.S.
Kropyvko, S.V.
Tsyba, L.O.
Gasman, S.
Rynditch, A.V.
author_sort Gubar, O.S.
title Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
title_short Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
title_full Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
title_fullStr Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
title_full_unstemmed Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
title_sort alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells
publisher Інститут молекулярної біології і генетики НАН України
publishDate 2012
topic_facet Structure and Function of Biopolymers
url http://dspace.nbuv.gov.ua/handle/123456789/156729
citation_txt Alternatively spliced short and long isoforms of adaptor protein intersectin 1 form complexes in mammalian cells / O.S. Gubar, S.V. Kropyvko, L.O. Tsyba, S. Gasman, A.V. Rynditch // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 429-423. — Бібліогр.: 29 назв. — англ.
series Вiopolymers and Cell
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first_indexed 2023-05-20T17:50:27Z
last_indexed 2023-05-20T17:50:27Z
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