Potentiality of application of the conductometric L-arginine biosensors for the real sample analysis

• Дата: 2012
• Автори: Saiapina, O.Y., Dzyadevych, S.V., Jaffrezic-Renault, N.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2012
• Назва видання: Вiopolymers and Cell
• Теми: Molecular and Cell Biotechnologies
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/156756
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Potentiality of application of the conductometric L-arginine biosensors for the real sample analysis / O.Y. Saiapina, S.V. Dzyadevych, N. Jaffrezic-Renault // Вiopolymers and Cell. — 2012. — Т. 28, № 6. — С. 441-448. — Бібліогр.: 27 назв. — англ.

Aim. To determine an influence of serum components on the L-arginine biosensor sensitivity and to formulate practical recommendations for its reliable analysis. Methods. The L-arginine biosensor comprised arginase and urease co-immobilized by cross-linking. Results. The biosensor specificity was investigated based on a series of representative studies (namely, through urea determination in the serum; inhibitory effect studies of mercury ions; high temperature treatment of sensors; studying the biosensor sensitivity to the serum treated by enzymes, and selectivity studies). It was found that the response of the biosensor to the serum injections was determined by high sensitivity of the L-arginine biosensor toward not only to L-arginine but also toward two other basic amino acids (L-lysine and L-histidine). Conclusions. A detailed procedure of optimization of the conductometric biosensor for L-arginine determination in blood serum has been proposed. Keywords: L-arginine, conductometric biosensors, serum, optimization procedure.