Expression of Arabidopsis gdh2 gene depends on activity of alternative electron transfer pathway in mitochondria

• Main Authors: Tarasenko, V.I., Garnik, E.Yu., Konstantinov, Yu.M.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2012
• Series: Вiopolymers and Cell
• Subjects: Genomics, Transcriptomics and Proteomics
• Online Access: http://dspace.nbuv.gov.ua/handle/123456789/156890

Aim. We studied the expression level of gdh2 gene, encoding subunit of mitochondrial glutamate dehydrogenase, in Arabidopsis suspension culture cells with genetically modified level of alternative oxidase AOX1a. Methods. Polymerase chain reaction, Northern-blotting. Results. The treatment with main electron transfer pathway inhibitor antimycin A led to an increase in gdh2 transcript level in the wild-type cells and the cells with decreased level of alternative oxidase, but not in the cells with elevated level of alternative oxidase. Conclusions. It is known that an increase in alternative oxidase level in the plant cell results in more oxidized state of ubiquinone pool in respiratory chain. Therefore, the obtained results support the earlier proposed model according to which the expression level of gdh2 gene depends on the redox state of ubiquinone pool. Keywords: Arabidopsis thaliana, electron transport chain, alternative oxidase, glutamate dehydrogenase, antimycin A.