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Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior

Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior

Our aim was to investigate the influence of some exogenous agents on the recombinant protein accumulation in plants via Agrobacterium-mediated transient expression. Methods. Agrobacterium-mediated transient expression method, spectrophotometric methods for protein analysis, statistical calculations....

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Main Authors: Sindarovska, Y.R., Gerasymenko, I.M., Kuchuk, M.V., Sheludko, Y.V.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2012
Series:Вiopolymers and Cell
Subjects:
Genomics, Transcriptomics and Proteomics
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/156891
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spelling irk-123456789-1568912019-06-20T01:26:45Z Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior Sindarovska, Y.R. Gerasymenko, I.M. Kuchuk, M.V. Sheludko, Y.V. Genomics, Transcriptomics and Proteomics Our aim was to investigate the influence of some exogenous agents on the recombinant protein accumulation in plants via Agrobacterium-mediated transient expression. Methods. Agrobacterium-mediated transient expression method, spectrophotometric methods for protein analysis, statistical calculations. Results. It was shown that the tested compounds in different concentrations (namely, auxins, cytokinin, methyl jasmonate and suppressors of jasmonate biosynthesis (phenidon and diethyldithiocarbamic acid)) added to the infiltration buffer did not influence either GFP transient expression nor total protein accumulation in plant tissue. Conclusions. The results suggest that the tested factors are not substantial for Agrobacterium-mediated transient expression. Keywords: Nicotiana excelsior, Agrobacterium, transient expression, GFP. Мета. Дослідження впливу деяких екзогенних агентів на накопичення рекомбінантного білка в рослинах за допомогою Agrobacterium-опосередкованої транзієнтної експресії. Методи. Agrobacterium-опосередкована транзієнтна експресія, спектрофотометричні методи для аналізу білків, статистичні розрахунки. Результати. Показано, що протестовані речовини, а саме – деякі концентрації ауксинів, цитокініну, метилжасмонату і супресорів його біосинтезу (фенідону і диетилдитіокарбамату), додані до інфільтраційного буфера, не впливають на транзієнтну експресію GFP і загальний рівень вмісту білка в рослинній тканині. Висновки. Результати свідчать, що протестовані фактори не є суттєвими для проведення Agrobacterium-опосередкованої транзієнтної експресії. Ключові слова: Nicotiana excelsior, Agrobacterium, транзієнтна експресія, GFP. Цель. Исследование влияния некоторых экзогенных агентов на накопление рекомбинантного белка в растениях с помощью Agrobacterium-опосредованной транзиентной экспрессии. Методы. Agrobacterium-опосредованная транзиентная экспрессия, спектрофотометрические методы для анализа белков, статистические расчеты. Результаты. Показано, что тестируемые вещества, а именно – некоторые концентрации ауксинов, цитокинина, метилжасмоната и супрессоров его биосинтеза (фенидона и диэтилдитиокарбамата), добавленные к инфильтрационному буферу, не влияют на транзиентную экспрессию GFP и общий уровень содержания белка в растительной ткани. Выводы. Результаты свидетельствуют, что тестируемые факторы не являются существенными для проведения Agrobacterium-опосредованной транзиентной экспрессии. Ключевые слова: Nicotiana excelsior, Agrobacterium, транзиентная экспрессия, GFP. 2012 Article Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior / Y.R. Sindarovska, I.M. Gerasymenko, M.V. Kuchuk, Y.V. Sheludko // Вiopolymers and Cell. — 2012. — Т. 28, № 5. — С. 368-373. — Бібліогр.: 30 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.000072 http://dspace.nbuv.gov.ua/handle/123456789/156891 57.084.1: 582.926.2: 577.21 en Вiopolymers and Cell Інститут молекулярної біології і генетики НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Genomics, Transcriptomics and Proteomics
Genomics, Transcriptomics and Proteomics
spellingShingle Genomics, Transcriptomics and Proteomics
Genomics, Transcriptomics and Proteomics
Sindarovska, Y.R.
Gerasymenko, I.M.
Kuchuk, M.V.
Sheludko, Y.V.
Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior
Вiopolymers and Cell
description Our aim was to investigate the influence of some exogenous agents on the recombinant protein accumulation in plants via Agrobacterium-mediated transient expression. Methods. Agrobacterium-mediated transient expression method, spectrophotometric methods for protein analysis, statistical calculations. Results. It was shown that the tested compounds in different concentrations (namely, auxins, cytokinin, methyl jasmonate and suppressors of jasmonate biosynthesis (phenidon and diethyldithiocarbamic acid)) added to the infiltration buffer did not influence either GFP transient expression nor total protein accumulation in plant tissue. Conclusions. The results suggest that the tested factors are not substantial for Agrobacterium-mediated transient expression. Keywords: Nicotiana excelsior, Agrobacterium, transient expression, GFP.
format Article
author Sindarovska, Y.R.
Gerasymenko, I.M.
Kuchuk, M.V.
Sheludko, Y.V.
author_facet Sindarovska, Y.R.
Gerasymenko, I.M.
Kuchuk, M.V.
Sheludko, Y.V.
author_sort Sindarovska, Y.R.
title Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior
title_short Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior
title_full Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior
title_fullStr Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior
title_full_unstemmed Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior
title_sort influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on agrobacterium-mediated transient expression in nicotiana excelsior
publisher Інститут молекулярної біології і генетики НАН України
publishDate 2012
topic_facet Genomics, Transcriptomics and Proteomics
url http://dspace.nbuv.gov.ua/handle/123456789/156891
citation_txt Influence of exogenous phytohormones, methyl jasmonate and suppressors of jasmonate biosynthesis on Agrobacterium-mediated transient expression in Nicotiana excelsior / Y.R. Sindarovska, I.M. Gerasymenko, M.V. Kuchuk, Y.V. Sheludko // Вiopolymers and Cell. — 2012. — Т. 28, № 5. — С. 368-373. — Бібліогр.: 30 назв. — англ.
series Вiopolymers and Cell
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AT kuchukmv influenceofexogenousphytohormonesmethyljasmonateandsuppressorsofjasmonatebiosynthesisonagrobacteriummediatedtransientexpressioninnicotianaexcelsior
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first_indexed 2023-05-20T17:50:53Z
last_indexed 2023-05-20T17:50:53Z
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