Plasminogen/plasmin influence on platelet aggregation

• Дата: 2012
• Автори: Roka-Moya, Y.M., Zhernossekov, D.D., Grinenko, T.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2012
• Назва видання: Вiopolymers and Cell
• Теми: Structure and Function of Biopolymers
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/156892
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Plasminogen/plasmin influence on platelet aggregation / Y.M. Roka-Moya, D.D. Zhernossekov, T.V. Grinenko // Вiopolymers and Cell. — 2012. — Т. 28, № 5. — С. 352-356. — Бібліогр.: 18 назв. — англ., укр.

Aim. Determination of the influence of Glu- and Lys-plasminogen/ plasmin on the platelet aggregation induced by different agonists. Methods. Platelet aggregometry. Spectrophotometry. Results. We have shown the inhibitory effect of Lys-plasminogen on human platelet aggregation. The pro-enzyme action is related to the second wave of aggregation. The inhibitory effect of Lys-plasminogen possesses dosedependent manner in case of thrombin-induced aggregation. The inhibitory effect of pro-enzyme with ristocetin as an inductor has not been observed. Glu-plasminogen and plasmin do not influence on platelet aggregation under studied conditions. A serine protease inhibitor aprotinin does not change the inhibitory effect of Lys-plasminogen. Conclusions. The results proved the inhibitory effect of Lys-plasminogen on thrombin- and collagen-induced platelet aggregation. The absence of inhibitory effect of Lys-plasminogen in the case of ristocetin-induced aggregation leads us to conclusion, that GP Ib/IX is not involved into the inhibited aggregation pathway. The unchanged inhibitory effect of Lys-plasminogen in the presence of aprotinin excludes plasmin participation in the inhibitory phenomenon. Keywords: platelet aggregation, plasminogen, plasmin.