Colonization capacity and monitoring of the biocontrol agent Pseudomonas sp. IMBG163 on wheat roots

• Дата: 2004
• Автори: Kovalchuk, M.V., Lytvynenko, T.L., Kononuchenko, O.V., Voznyuk, T.M., Rymar, S.Yu., Negrutska, V.V., Kozyrovska, N.O.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2004
• Назва видання: Біополімери і клітина
• Теми: Молекулярна та клітинна біотехнології
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/157217
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Colonization capacity and monitoring of the biocontrol agent Pseudomonas sp. IMBG163 on wheat roots / M.V. Kovalchuk, T.L. Lytvynenko, O.V. Kononuchenko, T.M. Voznyuk, S.Yu. Rymar, V.V. Negrutska, N.O. Kozyrovska // Біополімери і клітина. — 2004. — Т. 20, № 6. — С. 530-534. — Бібліогр.: 32 назв. — англ.

The effect of Pseudomonas sp. IMBG163 on wheat seedlings (Triticum aestivum L. cv. Katyusha) was studied. This strain has been marked with the gus reporter gene in order to examine a colonization pattern and to monitor its maintenance on the plant roots. The strain IMBG163 significantly enhanced all biometric parameters measured: dry weight, height of shoots, length of roots. Co-culture of IMBG163 with the plant growth promoting bacterium Paenibacillus sp. IMBG156 did enhance the PGP effects. Detection of marked experimental bacterium on/into the plant have being performed with histochemical GUS assay and did not reveal the endophytic pattern of colonization. For monitoring of IMBG163 in the rhizosphere, a culture-independent method, ARDRA, has been used. A combination of conventional and molecular techniques showed IMBG163 to be effective and persistent colonizer of the wheat roots.