Colonization capacity and monitoring of the biocontrol agent Pseudomonas sp. IMBG163 on wheat roots

• Main Authors: Kovalchuk, M.V., Lytvynenko, T.L., Kononuchenko, O.V., Voznyuk, T.M., Rymar, S.Yu., Negrutska, V.V., Kozyrovska, N.O.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2004
• Series: Біополімери і клітина
• Subjects: Молекулярна та клітинна біотехнології
• Online Access: http://dspace.nbuv.gov.ua/handle/123456789/157217

The effect of Pseudomonas sp. IMBG163 on wheat seedlings (Triticum aestivum L. cv. Katyusha) was studied. This strain has been marked with the gus reporter gene in order to examine a colonization pattern and to monitor its maintenance on the plant roots. The strain IMBG163 significantly enhanced all biometric parameters measured: dry weight, height of shoots, length of roots. Co-culture of IMBG163 with the plant growth promoting bacterium Paenibacillus sp. IMBG156 did enhance the PGP effects. Detection of marked experimental bacterium on/into the plant have being performed with histochemical GUS assay and did not reveal the endophytic pattern of colonization. For monitoring of IMBG163 in the rhizosphere, a culture-independent method, ARDRA, has been used. A combination of conventional and molecular techniques showed IMBG163 to be effective and persistent colonizer of the wheat roots.