The Shine-Dalgarno hybrid during initiation of translation and elongation

• Дата: 2007
• Автори: Maraschio, D., La Teana, A., Volpe, P.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2007
• Назва видання: Біополімери і клітина
• Теми: Матеріали семінару
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/157528
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: The Shine-Dalgarno hybrid during initiation of translation and elongation / D. Maraschio, Teana La, P. Volpe // Біополімери і клітина. — 2007. — Т. 23, № 3. — С. 243-249. — Бібліогр.: 18 назв. — англ.

It was unknown whether a synthetic Shine-Dalgarno (SD) oligonucleotide labelled with ³²P at its 5'-end ([³²P]oct) would be able to reach the anti-SD sequence of 16S rRNA at the early stages of translation only or during elongation. To verify this, [³²P]oct was incubated with 30S ribosomal subunits (RSUs), 70S ribosomes and polysomes, separately, while the SD/anti-SD binding was checked in them through sucrose gradients. The anti-SD sequence resulted highly available in 30S RSUs and sufficiently available in ribosomes. In both 30S RSUs and ribosomes, the addition of a model 002 mRNA in equimolar proportions displaced [³²P]oct for about 50 %. However, in ribosomes the presence of initiation factors (IFs) and fMet-tRNA influence neither the binding of [³²P]oct nor the competition coming from mRNA. In polysomes, [³²P]oct was unable to hybridize the anti-SD sequence, in agreement with the hypothesis that mRNA and 16S rRNA are involved in the SD/anti-SD interaction also during elongation.