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Трансформація Camelina sativa методом in planta

Трансформація Camelina sativa методом in planta

Aims. The aim of the study was to develop an efficient method of genetic transformation of C. sativa in planta for further improvement of this plant species. Methods. Used a strain of Agrobacterium tumefaciens AGL1, containing vector construction pGH217 of the reporter gene в-glucuronidase (gus) c...

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Bibliographic Details
Main Authors: Бойчук, Ю.М., Баєр, О.О., Баєр, Г.Я., Рахметов, Д.Б., Блюм, Я.Б., Ємець, А.І.
Format: Article
Language:Ukrainian
Published: Інститут молекулярної біології і генетики НАН України 2015
Series:Фактори експериментальної еволюції організмів
Subjects:
Молекулярні та клітинні біотехнології
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/177491
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Summary:Aims. The aim of the study was to develop an efficient method of genetic transformation of C. sativa in planta for further improvement of this plant species. Methods. Used a strain of Agrobacterium tumefaciens AGL1, containing vector construction pGH217 of the reporter gene в-glucuronidase (gus) controlled 35S promoter of cauliflower mosaic virus and nos-terminator, and selective marker gene hpt. Results. It was found that almost 13 % of seeds obtained after transformation of C. sativa in planta method were resistant to the selective agent. The selective hygromycin concentration to pick up transgenic false flax lines is 20 mg/l. Conclusion. As a result of in planta transformation a hygromycin-resistant C. sativa seeds were produced. Molecular analysis confirmed the transgenic nature of selected lines. Keywords: transformation, C. sativa, in planta.

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