Модифікація астаксантином радіаційно-індукованої хромосомної нестабільності в соматичних клітинах людини in vitro
Aim. Investigation the influence of astaxanthin on the radiation-induced cytogenetic effect in human peripheral blood lymphocytes. Methods. Astaxanthin in optimal concentration (20.0 μg/ml), established in the previous own research, added to culture of human peripheral blood lymphocytes prior to i...
Збережено в:
| Дата: | 2016 |
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| Автор: | |
| Формат: | Стаття |
| Мова: | Ukrainian |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
2016
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| Назва видання: | Фактори експериментальної еволюції організмів |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/177616 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Модифікація астаксантином радіаційно-індукованої хромосомної нестабільності в соматичних клітинах людини in vitro / Д.А. Курінний // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2016. — Т. 18. — С. 236-238. — Бібліогр.: 11 назв. — укр. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Резюме: | Aim. Investigation the influence of astaxanthin on the radiation-induced cytogenetic effect in human peripheral blood
lymphocytes. Methods. Astaxanthin in optimal concentration (20.0 μg/ml), established in the previous own research,
added to culture of human peripheral blood lymphocytes prior to incubation before gamma irradiation in dose 1 Gy.
Cultivation of peripheral blood lymphocytes received from five conditionally healthy volunteers during 48 hours; staining
of metaphase chromosome slides; scoring of slides under the microscope (cytogenetic analysis); identification of the full
range of chromatid and chromosome aberration types had been fulfilled. Results. Established that meangroup frequency
of chromosome aberrations which were presented only by acentrics was 2.57 ± 0.35 per 100 metaphases in intact cultures
and 2.36 ± 0.38 per 100 metaphases under astaxanthin exposure. After irradiation the total frequency of chromosome
aberrations increased to 24.47 ± 1.73 per 100 metaphases due to raising the level of simple acentrics (7.29 ± 1.05 per 100
metaphases) and induction the exchanges – dicentrics, centric rings and abnormal monocentrics (12.80 ± 1.35; 2.75 ±
0.66; 0.49 ± 0.28 per 100 metaphases, respectively). The treatment of irradiated human peripheral blood lymphocytes by
astaxanthin led to significant decrease the frequency of chromosome aberrations (8.40 ± 0.90 per 100 metaphases) due to
decline of unstable cytogenetic markers of radiation exposure – dicentrics and centric rings (1.90 ± 0.44 and 0.42 ± 0.21
per 100 metaphases, respectively) and reduction the total frequency of double fragments and acentric rings (5.28 ± 0.82
per 100 metaphases). Conclusions. Data obtained testify about powerful genoprotective and radioprotective potential of
astaxanthin in studied concentration.
Keywords: astaxanthin, frequency of chromosome aberrations, human peripheral blood lymphocytes, gamma irradiation. |
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