Дифференцированная циркадианная реакция на свет, проявляемая Т клетками и большими гранулярными лимфоцитами человека in vitro
Recently we have shown that rhythmic daily fluctuations of the human blood lymphocyte ability to react with sheep erythrocytes (ER activity) persisted in lymphocytes placed in culture under the dark conditions or day-time illumination. If the illumination was switched over to the night- time, the...
Збережено в:
| Видавець: | Видавничий дім "Академперіодика" НАН України |
|---|---|
| Дата: | 2007 |
| Автори: | , |
| Формат: | Стаття |
| Мова: | Russian |
| Опубліковано: |
Видавничий дім "Академперіодика" НАН України
2007
|
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/3146 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Цитувати: | Дифференцированная циркадианная реакция на свет, проявляемая Т клетками и большими гранулярными лимфоцитами человека in vitro / Н.Ф. Гамалея, Е.Д. Шишко // Доп. НАН України. — 2007. — № 9. — С. 131-136. — Бібліогр.: 15 назв. — рос. |
Репозиторії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Резюме: | Recently we have shown that rhythmic daily fluctuations of the human blood lymphocyte ability
to react with sheep erythrocytes (ER activity) persisted in lymphocytes placed in culture under
the dark conditions or day-time illumination. If the illumination was switched over to the night-
time, the inversion of this circadian rhythm was observed. The results implied that lymphocytic
cells contained a self-sustained light-responsive circadian clock. As ER activity is characteristic
of two lymphocyte subpopulations in human blood-T cells and large granular lymphocytes (LGL),
we now studied the found circadian clock in enriched fractions of these lymphocyte subpopulati-
ons, isolated and maintained in culture separately. It was established that although circadian
variations of the lymphocyte activity were evident in both cell fractions, the rhythms turned
out light-entrainable only in the LGL one. However, when the two lymphocyte subpopulati-
ons were grown in a double-chambered vessel which allowed humoral exchange between them,
T cell rhythms also became light-responsive, suggesting that a difusible element of the photo-
receptive/circadian system could be transferred from LGL onto T lymphocytes through a half-
permeable membrane. The findings are discussed in the context of an apparent key role of the
LGL in the proposed mechanism of non-visual circadian photoregulation in man. |
|---|