Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer
The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substituti...
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| Дата: | 2009 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
2009
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/5651 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
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irk-123456789-56512010-02-02T12:01:22Z Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. Структура і функції біополімерів The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease. 2009 Article Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ. 0233-7657 http://dspace.nbuv.gov.ua/handle/123456789/5651 577.2, 577.27 en Інститут молекулярної біології і генетики НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Структура і функції біополімерів Структура і функції біополімерів |
| spellingShingle |
Структура і функції біополімерів Структура і функції біополімерів Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| description |
The aim of present study was to develop a model for a functional analysis of a recently identified marker of the ovarian cancer - sodium-dependent phosphate transporter NaPi2b. For this purpose, we have created HEK293 stable cell lines expressing wild type or mutant forms of NaPi2b (T330V substitution in a large extracellular loop and a 6 amino acid residues deletion in the C-terminal cytoplasmic tail), revealed in the ovarian cancer cell lines. The expression of wild type and mutant forms NaPi2b in the stable cell lines created was confirmed by Western-blot analysis with monoclonal antibodies against NaPi2b. The cellular models described here will be useful for studying the function of sodium-dependent phosphate transporter NaPi2b in health and disease. |
| format |
Article |
| author |
Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. |
| author_facet |
Gryshkova, V.S. Lituyev, D.S. Filonenko, V.V. Kiyamova, R.G. |
| author_sort |
Gryshkova, V.S. |
| title |
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_short |
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_full |
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_fullStr |
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_full_unstemmed |
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer |
| title_sort |
creation of cellular models for the analysis of sodium-dependent phosphate transporter napi2b, a potential marker for ovarian cancer |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| publishDate |
2009 |
| topic_facet |
Структура і функції біополімерів |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/5651 |
| citation_txt |
Creation of cellular models for the analysis of sodium-dependent phosphate transporter NaPi2b, a potential marker for ovarian cancer / V.S. Gryshkova, D.S. Lituyev, V.V. Filonenko, R.G. Kiyamova // Біополімери і клітина. — 2009. — Т. 25, № 2. — С. 95-100. — Бібліогр.: 23 назв. — англ. |
| work_keys_str_mv |
AT gryshkovavs creationofcellularmodelsfortheanalysisofsodiumdependentphosphatetransporternapi2bapotentialmarkerforovariancancer AT lituyevds creationofcellularmodelsfortheanalysisofsodiumdependentphosphatetransporternapi2bapotentialmarkerforovariancancer AT filonenkovv creationofcellularmodelsfortheanalysisofsodiumdependentphosphatetransporternapi2bapotentialmarkerforovariancancer AT kiyamovarg creationofcellularmodelsfortheanalysisofsodiumdependentphosphatetransporternapi2bapotentialmarkerforovariancancer |
| first_indexed |
2023-03-24T08:34:01Z |
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2023-03-24T08:34:01Z |
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