Investigating the effects of vitreous humour (crude extract) on growth and differentiation of rat mesenchymal stem cells (rMSCs) and human NTERA2 cells

• Дата: 2010
• Автори: Bagherpoor, A.J., Bahrami, A.R., Matin, M.M., Mahdavi-Shahri, N., Edalatmanesh, M.A.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут клітинної біології та генетичної інженерії НАН України 2010
• Назва видання: Цитология и генетика
• Теми: Оригинальные работы
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/66802
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Investigating the effects of vitreous humour (crude extract) on growth and differentiation of rat mesenchymal stem cells (rMSCs) and human NTERA2 cells / A.J. Bagherpoor, A.R. Bahrami, M.M. Matin, N. Mahdavi-Shahri, M.A. Edalatmanesh // Цитология и генетика. — 2010. — Т. 44, № 5. — С. 15-21. — Бібліогр.: 22 назв. — англ.

It is very well documented that retinoic acid (RA) reduces growth rate by induction of cell differentiation in certain conditions and cell lines. On the other hand, hyaluronic acid (HA) is known for its growth induction on cultured cells. A natural source of HA, rabbit vitreous humour (VH), was previously shown to promote wound repair in model animals. In search for its possible mechanisms, VH extract was tested on the cultured mesenchymal stem cells and NTERA2 as human embryonal carcinoma cells in the presence of RA. Changes in some cellular and molecular markers (A2B5, Oct4, Sox2) showed that VH and possibly HA interfere with differentiating effects of RA. Therefore, this reagent may affect cell proliferation and tissue regeneration by inhibition of cell differentiation.