BIOTRANSFORMATION OF HYDROCARBONS IN THE GROUND SINKING

• Date: 2016
• Main Authors: Shkapenko, V.V., Kadoshnikov, V.M., Musich, E.G., Pisanskaya, I.R.
• Format: Article
• Language: Russian
• Published: Institute of Geological Sciences, NAS of Ukraine 2016
• Subjects: hydrocarbons; petroleum products; transformation; biodestructors; microorganisms
• Online Access: http://geojournal.igs-nas.org.ua/article/view/97273
• Journal Title: Geological journal

The processes of biotransformation of non-polar hydrocarbons are examined in the article. The ground sinking of off-shore area of north part of the Black sea was probed. The standards of the ground fallouts were taken away in the Sevastopol bay and in the off-shore area of Karadagsky of natural preserve. Mineral composition of sandy faction of the selected standards is presented kalcitom and kvarcem with the negligible quantity of pelitic faction (no more than 2%).  The standards of silt deposits mineral composition of which was presented montmorillonitom were taken away in deep-water part of the Sevastopol bay, illitom, kaolinite and vysokodispersnym kal'citom and kvarcem, and also by the particles of feldspars (analcin).The organic matter of silt deposits was presented humic acids (3%) and oil hydrocarbons (0,25%). A betweenness by humic acids and oil-products in the ground sinking of the Sevastopol bay grounds to consider that under the action of microorganisms oil-products is transformed in humic acids. This conclusion is confirmed the results of laboratory researches. For the design of this process was developed and prepared series of preparations, containing hydrocarbons mineral phase and bio-preparation in different correlations. The got mixture was maintained in a thermostat at permanent humidity 60-70% and to the temperature of 37±1oS is 21 day. It was set by the method of IK-spectroscopy, that as a result of action of microorganisms, containing cyclic structures which are identified as benzol rings are in aromatic hydrocarbons, appear in a chloroform extract. It is expressed appearance of bars of absorption in area of 1170 sm-1 and 1270 sm-1, on the IK-spectrums of standards additionally bars in area of 1715 sm-1 and 1735 sm-1 appear proper the valency vibrations of S=O, that characteristically for the processes of oxidization on spectrums. Essential influence on the process of transformation of hydrocarbons is rendered by the ions of ammonium, which in the conditions of lack of oxygen, promotes in anaerobic oxidization of hydrocarbons nitrates and nitrites with formation of ammonium. Restoration amination of intermediate oxidates hydrocarbons with formation of the nitrogenated organic compounds, including amino acid, assumes possibility of including of surplus carbon in the complement of organic matter. Being of high- dispersion mineral phase in the ground sinking accelerates the process of mineralizacii and partly results in the accumulation of guminosimilar polymers. It is experimentally well-proven that only during the certain concentration of clay minerals in a cultural environment it is possible to attain optimum terms for transformation of hydrocarbons.  The special composition, containing the complex of microorganisms and bentonito's clay allowing effectively to remove oil contaminations from mineral surfaces, is developed. The offered composition can be used for cleaning of hard mineral surfaces of both natural and technogenic objects.