Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine
Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud, 1993 previously known from France and Bulgaria is reported from Ukraine for the first time. The species was found in the material from 8 amphibian host species, of which Lissotriton montadoni, Triturus cristatus, Mesotriton alpestris, Pel...
Saved in:
| Published in: | Вестник зоологии |
|---|---|
| Date: | 2012 |
| Main Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут зоології ім. І.І. Шмальгаузена НАН України
2012
|
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/109372 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine / R.S. Svitin, Y.I. Kuzmin // Вестник зоологии. — 2012. — Т. 46, № 3. — С. 195-203. — Бібліогр.: 18 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859997941536653312 |
|---|---|
| author | Svitin, R.S. Kuzmin, Y.I. |
| author_facet | Svitin, R.S. Kuzmin, Y.I. |
| citation_txt | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine / R.S. Svitin, Y.I. Kuzmin // Вестник зоологии. — 2012. — Т. 46, № 3. — С. 195-203. — Бібліогр.: 18 назв. — англ. |
| collection | DSpace DC |
| container_title | Вестник зоологии |
| description | Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud, 1993 previously known from France and Bulgaria is reported from Ukraine for the first time. The species was found in the material from 8 amphibian host species, of which Lissotriton montadoni, Triturus cristatus, Mesotriton alpestris, Pelophylax ridibunda, P. lessonae, and Hyla arborea are new host records. Newts (Salamandridae) and green frogs (Pelophylax) are considered to be typical hosts for O. duboisi. Illustrated morphological redescription of O. duboisi based on 141 specimens from various hosts is presented.
Вид Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud, 1993, ранее известный из Франции и Болгарии, впервые обнаружен в Украине. Вид найден в материале от 8 видов амфибий, из которых Lissotriton montadoni, Triturus cristatus, Mesotriton alpestris, Pelophylax ridibunda, P. lessonae и Hyla arborea являются новыми хозяевами O. duboisi. Предполагается, что основными хозяевами O. duboisi являются тритоны (Salamandridae) и зеленые лягушки (род Pelophylax). Представлено иллюстрированное описание O. duboisi по 141 изученному экземпляру от разных видов хозяев.
|
| first_indexed | 2025-12-07T16:35:00Z |
| format | Article |
| fulltext |
UDC 595.132
OSWALDOCRUZIA DUBOISI (NEMATODA, MOLINEIDAE):
MORPHOLOGY, HOSTS AND DISTRIBUTION IN UKRAINE
R. S. Svitin1, Y. I. Kuzmin2
1 Taras Shevchenko National University of Kiev, Educational and Scientific Centre “Institute of Biology”, 2
Glushkova prosp., Kyiv, 01601 Ukraine
E-mail: romasvit@bigmir.net
2 Schmalhausen Institute of Zoology of the NAS of Ukraine,
B. Chmielnicky str., 15, Kiev, 01601 Ukraine
E-mail: rhabdias@izan.kiev.ua
Received 8 February 2012
Accepted 29 March 2012
Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine.
Svitin R. S., Kuzmin Y. I. – Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud, 1993 pre-
viously known from France and Bulgaria is reported from Ukraine for the first time. The species was
found in the material from 8 amphibian host species, of which Lissotriton montadoni, Triturus cristatus,
Mesotriton alpestris, Pelophylax ridibunda, P. lessonae, and Hyla arborea are new host records. Newts
(Salamandridae) and green frogs (Pelophylax) are considered to be typical hosts for O. duboisi.
Illustrated morphological redescription of O. duboisi based on 141 specimens from various hosts is pre-
sented.
Ke y wo r d s: Oswaldocruzia, O. duboisi, Amphibia, Ukraine, synlophe, cervical alae.
Oswaldocruzia duboisi (Nematoda, Molineidae): ìîðôîëîãèÿ, õîçÿåâà è ðàñïðîñòðàíåíèå â Óêðàèíå.
Ñâèòèí Ð. Ñ., Êóçüìèí Þ. È. – Âèä Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud,
1993, ðàíåå èçâåñòíûé èç Ôðàíöèè è Áîëãàðèè, âïåðâûå îáíàðóæåí â Óêðàèíå. Âèä íàéäåí â
ìàòåðèàëå îò 8 âèäîâ àìôèáèé, èç êîòîðûõ Lissotriton montadoni, Triturus cristatus, Mesotriton
alpestris, Pelophylax ridibunda, P. lessonae è Hyla arborea ÿâëÿþòñÿ íîâûìè õîçÿåâàìè O. duboisi.
Ïðåäïîëàãàåòñÿ, ÷òî îñíîâíûìè õîçÿåâàìè O. duboisi ÿâëÿþòñÿ òðèòîíû (Salamandridae) è çåëå-
íûå ëÿãóøêè (ðîä Pelophylax). Ïðåäñòàâëåíî èëëþñòðèðîâàííîå îïèñàíèå O. duboisi ïî 141 èçó-
÷åííîìó ýêçåìïëÿðó îò ðàçíûõ âèäîâ õîçÿåâ.
Êëþ÷åâûå ñ ëîâ à: Oswaldocruzia, O. duboisi, Amphibia, Óêðàèíà, ñèíëîô, ëàòåðàëüíûå êðûëüÿ.
Introduction
Nematodes of the genus Oswaldocruzia Travassos, 1917 are worldwide distributed intestine parasites of
amphibians and reptiles. Presently, more than 80 species are assigned to the genus (Schotthoefer et al., 2009).
In Western Palaearctic, most species of Oswaldocruzia are morphologically similar and were often iden-
tified as Oswaldocruzia filiformis Goeze, 1782 parasitizing amphibians (Rana temporaria, R. arvalis, R. ridi-
bunda, R. lessonae, R. esculenta, Bufo bufo, B. viridis, Hyla arborea, Bombina bombina, B. varigeata, Pelobates
fuscus, Salamandra salamandra, Tritirus vulgaris, T. cristatus), reptiles of the genera Anguis, Lacerta, Natrix,
Ophisaurus, Vipera, and even fish Lotta lotta (Lal, 1944; Ryzhikov et al., 1980; Baker, 1981; Anderson, 2000;
Galli et al., 2001; Odnokurtsev and Sedalishchev, 2008; Novokhatskaya, 2008).
Five European species of the genus were described and differentiated using the structure of synlophe
and spicules: O. duboisi Ben Slimane, Durette-Desset et Chabaud, 1993 from Rana sp., R. dalmatica, Triturus
vulgaris in France; O. guyetanti Ben Slimane, Durette-Desset et Chabaud, 1993 from Rana sp. and R. tem-
poraria in France; O. hispanica Ben Slimane, Lluch et Durette-Desset, 1995 from R. temporaria in Spain;
O. galeanoae Ben Slimane, Lluch et Durette-Desset, 1995 from Bufo bufo in Spain; O. bialata Molin, 1860
from R. temporaria in Italy and from Bufo sp. in Bulgaria (Ben Slimane et al., 1993, 1995).
Structure of the synlophe is unknown in other European Oswaldocruzia spp.: O. ukrainae Ivanitzky,
1940; O. ivanizkii Sudarikov, 1951, O. fulleborni Iwanitzky, 1940, O. problematica Ivanitsky, 1940, O. molge-
Vestnik zoologii, 46(3): e-1—e-9, 2012
DOI 10.2478/v10058-012-0017-x
Ôàóíà è ñèñòåìàòèêà
Unauthenticated
Download Date | 11/25/16 10:02 PM
ta Lewis, 1928, though they were included as valid species in the list of Palaearctic species of the genus
Oswaldocruzia by Ben Slimane et al. (1995).
Of 10 species of Oswaldocruzia found in Western Palaearctic, 5 were previously reported from Ukraine:
O. filiformis, O. ukrainae, O. ivanizkii, O. fulleborni, and O. problematica (Skrjabin et al., 1954; Ryzhikov et
al., 1980). At least some species described in Western Europe after 1980 presumably occur in Ukraine as well,
since their hosts are widespread in Western Palaearctic.
During the investigation of the helminthological material stored in the collection of the Department of
Parasitology of the Institute of Zoology, NAS of Ukraine we found several Oswaldocruzia species from
amphibian hosts, including O. duboisi from various newts and frogs from Ukraine. This species was identi-
fied based on characteristic synlophe structure. It was first found in France and described based on several
specimens; the host range of the species was not exactly identified (Rana sp., R. dalmatina and Triturus vul-
garis) (Ben Slimane et al., 1993). Thereafter, the species was reported only once as parasite of Rana escu-
lenta in Bulgaria (Durette-Desset et al., 1993). Our studies of more than 100 specimens of O. duboisi from
various host species added some information to the morphology, distribution and specificity of the species.
This information is presented herein.
Material and methods
Material from the collection of the Department of Parasitology of the Institute of Zoology, NAS of
Ukraine was investigated. Totally, 141 specimens of O. duboisi were studied, of which 43 males and
41 females were measured: 7 males and 3 females from P. ridibunda, 4 males and 8 females from
P. lessonae, 3 males and 2 females from P. esculenta, 4 males and 12 females from M. alpestris, 10 males and
7 females from L. montadoni, 13 males and 11 females from L. vulgaris, 2 males from T. cristatus.
All specimens were fixed and stored in 4% solution of formalin in saline. Prior to examination, nema-
todes were cleared in phenol-glycerine solution (2 : 1 ratio). After the studies, all nematodes were placed in
70% alcohol for further storage.
Morphology of nematodes was studied under the light microscope Zeiss Axio Lab. All measurements
in the text are given in micrometers unless otherwise indicated. Figures were made using the drawing tube
RA-7. Photomicrographs were made using Zeiss Axio Imager M1 system.
The study of the synlophe is based on the method of Durette-Desset (1985). The nomenclature of the
synlophe in the oesophageal region follows that of Ben-Slimane et al. (1993). The nomenclature of the cau-
dal bursa follows Durette-Desset and Chabaud (1981). Nomenclature of amphibians follows Pisanets (2007).
Results
Oswaldocruzia duboisi Ben-Slimane, Durette-Desset et Chabaud, 1993
Ho s t s. Salamandridae: Lissotriton vulgaris, L. montadoni, Triturus cristatus,
Mesotriton alpestris; Ranidae: Pelophylax ridibunda, P. lessonae, P. kl. esculenta;
Hylidae: Hyla arborea.
S i t e: intestine, stomach.
Distribution in Ukraine: Ivano-Frankivska oblast (8 localities), Kyivska oblast
(2 localities), and Crimea (2 localities) (fig. 1).
De s c r i p t i o n. General. In both sexes, body thin, elongated, with maximum
width near midlength. Anterior end rounded. Oral opening triangular; 4 externo-labial
papillae, 4 cephalic papillae and 2 amphids present (fig. 2, 4). Body cuticle thin. On
anterior end, cuticle forming cephalic vesicle. Vesicle undivided (fig. 2, 1) or consist-
ing of two parts: anterior part rounded and smooth; posterior part less inflated, smooth
or with transverse folds (fig. 2, 2, 3). Only divided cephalic vesicle was observed in
specimens from L. vulgaris (tables 3, 4).
Oesophagus club-shaped, cylindrical in anterior half, then widening posteriorly,
with oval posterior bulb (fig. 2, 1). Position of excretory pore variable, but not beyond
level of posterior third of oesophagus. Two excretory glands dissimilar in size, both
glands somewhat longer than oesophagus. Nerve ring encircling oesophagus near its
midlength, often closer to its anterior third. Minute deirids situated at level of
oesophageal-intestinal junction.
Synlophe symmetrical. Lateral cervical alae present, each beginning slightly poste-
rior to cephalic vesicle and transforming into one simple crest posterior to level of
oesophageal-intestinal junction. On transverse sections (fig. 2, 5, 6; 3, 3, 4), alae tri-
e-2 R. S. Svitin, Y. I. Kuzmin
Unauthenticated
Download Date | 11/25/16 10:02 PM
angular, with rounded top, longer dorsal side and shorter, often concave ventral side.
Struts in alae present, but usually poorly visible. Two crests present on dorsal side of
each ala closer to its base. Crest situated at base of ala more prominent, other one
sometimes indistinct. Maximum height of cervical alae at level of oesophageal-intesti-
nal junction. Ventral crests always present on sections at mid-oesophagus level. Number
of crests in oesophageal region varying depending on distance from anterior end: from
22 to 33 crests including cervical alae. Seventy-one equal crests present at mid-body
level (fig. 2, 7).
Ma l e s (fig. 2, 10—12; 3, 1, 2; tables 1, 3). Cervical alae about 530 long, 10 height,
beginning at about 180 from anterior end of body. Testis extending along entire troph-
icogenital part of body. Caudal bursa (fig. 2, 12; 3, 2) of type II. Rays 2 and 3 joined
along their whole length, ray 4 joined to ray 5 in its proximal half, rays 5 and 6 joined
along their whole length, rays 6 and 8 jointed in region of midlength. Dorsal ray (fig. 2,
11) bifurcated into two rays 10 posterior to base of rays 9.
Spicules (fig. 2, 10) equal, with three branches: distally sharpened blade, fork
divided in two branches at level of posterior third, and shoe with thin process. All
branches of same length and without extra branches. Spicule length varying significant-
ly in sample studied (table 1).
We did not observe obvious correlation between the length of spicules and the body
size, or between the length of spicules and host species. However, we found 5 males
from P. ridibunda in Crimea possessing markedly larger spicules (225—250 long) than
other studied males (spicules 155—210 long).
F ema l e s (fig. 2, 1, 8, 9; tables 2, 4). Larger than males. Posterior end sharpened.
Cervical alae about 648 long, 25 height, beginning at about 197 from anterior end of
body.
e-3Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine
Fig. 1. Finds of O. duboisi in Ukraine.
Ðèñ. 1. Íàõîäêè O. duboisi íà òåððèòîðèè Óêðàèíû.
Unauthenticated
Download Date | 11/25/16 10:02 PM
e-4 R. S. Svitin, Y. I. Kuzmin
Fig. 2. Oswaldocruzia duboisi: 1 – anterior part of the body, female from L. montadoni, right lateral view
(cephalic vesicle of type I); 2, 3 – variations of cephalic vesicle shape (types II and III), males from L. vul-
garis and L. montadoni, respectively; 4 – anterior end, apical view, male from P. lessonae; 5 – transverse
section at mid-oesophagus level, male from L. vulgaris (c – crests on dorsal side of cervical ala) ; 6 – trans-
verse section at posterior part of oesophagus, female from P. lessonae; 7 – transverse section at mid-body,
female from P. ridibunda; 8 – posterior end, female from P. ridibunda, left lateral view; 9 – ovejector and
vulva, right lateral view, female from P. ridibunda; 10 – right spicule, left lateral view, male from L. vulgaris;
11 – dorsal ray of the caudal bursa, male from L. vulgaris; 12 – caudal bursa, ventral view, male from L. vul-
garis. Scale bars: 1—3, 5—10,12 – 0.1 mm; 4, 11 – 0.05 mm.
Ðèñ. 2. Oswaldocruzia duboisi: 1 – ïåðåäíÿÿ ÷àñòü òåëà ñàìêè èç L. montadoni, ëàòåðàëüíî (ãîëîâíàÿ âåçè-
êóëà òèïà I); 2, 3 – ðàçëè÷èÿ â ñòðîåíèè ãîëîâíîé âåçèêóëû, ñàìöû èç L. vulgaris è L. montadoni ñîîò-
âåòñòâåííî (ãîëîâíàÿ âåçèêóëà òèïà II è III); 4 – ïåðåäíèé êîíåö, àïèêàëüíî, ñàìåö èç P. lessonae;
5 – ïîïåðå÷íûé ñðåç òåëà íà óðîâíå ñåðåäèíû äëèíû ïèùåâîäà, ñàìåö èç L. vulgaris (ñ – ãðåáíè íà
äîðñàëüíîé ñòîðîíå ëàòåðàëüíîãî êðûëà); 6 – ïîïåðå÷íûé ñðåç òåëà íà óðîâíå çàäíåãî êîíöà ïèùå-
âîäà, ñàìêà èç P. lessonae; 7 – ïîïåðå÷íûé ñðåç íà óðîâíå ñåðåäèíû òåëà, ñàìêà èç P. ridibunda; 8 –
õâîñò ñàìêè èç P. ridibunda, ëàòåðàëüíî; 9 – ÿéöåìåò è âóëüâà, ëàòåðàëüíî, ñàìêà èç P. ridibunda; 10 –
ïðàâàÿ ñïèêóëà, âèä ñëåâà, ñàìåö èç L. vulgaris; 11 – äîðñàëüíàÿ âåòâü ïîëîâîé áóðñû, ñàìåö èç L. vul-
garis; 12 – ïîëîâàÿ áóðñà, ñàìåö èç L. vulgaris. Ìàñøòàá: 1—3, 5—10,12 – 0,1 ìì; 4,11 – 0,05 ìì.
Unauthenticated
Download Date | 11/25/16 10:02 PM
e-5Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine
Ta b l e 1. Morphometry of O. duboisi males, 43 specimens (SD – standard deviation, CV – coefficient of
variation)
Ò à á ëèö à 1. Ìîðôîìåòðèÿ O. duboisi, ñàìöû, 43 ýêç. (SD – ñòàíäàðòíîå îòêëîíåíèå, CV –
êîýôôèöèåíò âàðèàöèè)
Characters mean min. max. SD CV
Length of body 5626 3540 7280 843.0 14.98
Width of body 168 90 530 110.2 65.51
Length of cephalic vesicle 64 53 83 8.98 14.10
Width of cephalic vesicle 38 28 48 4.81 12.69
Length of oesophagus 396 283 583 58.13 14.69
Length of oesophagus in % of body length 7.11 4.76 9.89 1.09 15.37
Width of anterior end of oespohagus 24 19 28 2.02 8.42
Width of oespohagus at midlength 24 20 28 1.73 7.22
Width of oesophageal bulb 50 33 58 7.19 14.50
Distance to nerve ring from anterior end
of oesophagus
178 115 268 30.74 17.28
Distance to nerve ring in % of oesophagus length 45.05 35.40 57.75 5.31 11.79
Distance to excretory pore from anterior end
of oesophagus
260 198 348 36.47 14.04
Distance to excretory pore in % of esophagus length 66.08 49.07 87.42 9.49 14.36
Length of tail 118 98 168 17.57 14.88
Length of spicules 176 155 250 27.27 14.70
Length of spicules in % of body length 3.18 2.13 4.59 0.55 17.2
Ta b l e 2. Morphometry of O. duboisi females, 41 specimens
Ò à á ëèö à 2. Ìîðôîìåòðèÿ O. duboisi, ñàìêè, 41 ýêç.
Characters mean min. max. SD CV
Length of body 8887 2290 13620 2114 23.79
Width of body at midlength 173 100 250 28.38 16,43
Length of cephalic vesicle 71 50 95 10.24 14.49
Width of cephalic vesicle 44 38 50 3.61 8.27
Length of oesophagus 437 375 508 32.74 7.49
Length of oesophagus in % of body length 5.21 3.47 17.69 2.52 48.46
Width of anterior end of oespohagus 27 25 33 2.01 7.41
Width of oesophagus at midlength 27 23 33 2.24 8.46
Width of oesophageal bulb 206 148 273 32.44 15.74
Distance to nerve ring from anterior end
of oesophagus
189 160 245 21.70 11.47
Distance to nerve ring in % of oesophagus length 43.29 37.36 51.16 3.80 8.78
Distance to excretory pore from anterior end
of oesophagus
270 190 433 48.81 18.09
Distance to excretory pore in % of oesophagus
length
61.50 46.55 86.10 8.49 13.81
Distance from anterior end to vulva 5619 2760 8400 1102 19.61
Distance to vulva in % of body length 62.31 43.95 68.55 4.61 7.40
Length of tail 206 148 273 32.44 15.73
Length of tail in % of body length 2.42 1.67 7.42 1.03 42.68
Vulva wide, post-equatorial in position. Anterior ovary beginning near posterior
end of excretory glands and forming from 2 to 16 loops and bends depending on size
(age) of female*. Posterior ovary beginning anterior to vulva and forming 2—10 loops
and bends. Anterior and posterior uteri containing 4—59 and 0—52 eggs, respectively.
All eggs observed in uteri and ovejectors on morula stage. Length of anterior infundibu-
la 95—428, maximum width closer to uterus 68—95, minimum width closer to sphinc-
* Details of genital system shape and measurements are given based on 10 females studied.
Unauthenticated
Download Date | 11/25/16 10:02 PM
e-6 R. S. Svitin, Y. I. Kuzmin
Fig. 3. Oswaldocruzia duboisi, photomicrograps: 1 – male, general view; 2 – caudal bursa, ventral view; 3 –
transverse section at mid-oesophagus level; 4 – transverse section at posterior part of oesophagus (c – crests
on dorsal side of cervical ala). Scale bars: 1 – 1 mm; 2—4 – 0.1 mm.
Ðèñ. 3. Oswaldocruzia duboisi: 1 – îáùèé âèä ñàìöà; 2 – ïîëîâàÿ áóðñà, âåíòðàëüíî; 3 – ïîïåðå÷íûé
ñðåç òåëà íà óðîâíå ñåðåäèíû äëèíû ïèùåâîäà; 4 – ïîïåðå÷íûé ñðåç òåëà íà óðîâíå çàäíåãî êîíöà
ïèùåâîäà (ñ – ãðåáíè íà äîðñàëüíîé ñòîðîíå ëàòåðàëüíîãî êðûëà). Ìàñøòàá: 1 – 1 ìì; 2—4 –
0,1 ìì.
Unauthenticated
Download Date | 11/25/16 10:02 PM
ter 36—77. Length of posterior infundibula 189—446, maximum width 63—117, mini-
mum width 41—72. Anterior sphincter 36—77 long, 63—77 wide; posterior one
45—77 long and 59—90 wide. Length of anterior ovejector 248—338, maximum width
99—180, minimum width 63—144. Length of posterior ovejector 212—324, maximum
width 90—189, minimum width 59—171. Ovejectors with well-developed musculature.
Vagina 81—95 long and 5—9 in diameter. Egg size 95—117x157—162 (N = 21).
Tail (fig. 2, 8) tapering, elongated, with or without sharp cuticular needle on tip.
Discussion
In the present study, we found O. duboisi in 3 distant localities on the territory of
Ukraine: Ivano-Frankivska oblast in western Ukraine, Kyivska oblast in northern cen-
tral Ukraine, and Crimea, southern Ukraine. We suppose that real distribution of the
species in Ukraine is not so scattered, and it will be found in other localities in the fur-
ther studies. Previously O. duboisi was reported from France (Ben Slimane et al., 1993)
and Bulgaria (Durette-Dessetet al., 1993). Its occurrence in Ukraine means that the
species is rather widely distributed in Western Palaearctic. In our opinion, distribution
area of the species may be outlined by further studies and correct identification of
Oswaldocruzia from newts and green frogs.
Our studies confirmed that O. duboisi parasitize two distantly related groups of
hosts: newts L. vulgaris, L. montadoni, T. cristatus, M. alpestris (Caudata: Salaman -
dridae) and frogs Pelophylax spp. and Rana dalmatina (Anura: Ranidae). Lissotriton
montadoni, T. cristatus, and M. alpestris are new salamandrid hosts for O. duboisi.
Apperently, the observed specificity of O. duboisi is an example of ecological fitting
(Brooks et al., 2006), since its hosts from the both groups commonly share the same
fresh-water habitats (Shcherbak and Shcherban, 1980).
We also found O. duboisi in the material from 2 H. arborea (1 parasite specimen
in each host). Twelve other samples of parasites collected from H. arborea contained
other species of Oswaldocruzia clearly different from O. duboisi. In our opinion,
H. arborea is not a specific host of O. duboisi, though it may be occasionally infected
by this nematode.
Oswaldocruzia duboisi is morphologically similar and closely related to O. filiformis
(Ben Slimane et al., 1995). The latter species was reported from a wide range of hosts
in Western Palaearctic, including newts (Salamandridae) and green frogs (Pelophylax,
Ranidae) (Ryzhikov et al., 1980; Odnokurtsev and Sedalishchev, 2008; Yildrimhan,
2008). In our opinion, some records might be results of misidentification, and the
authors in fact dealt with O. duboisi. In our studies, we did not find O. filiformis in the
material from newts and green frogs from Ukraine. The species was found only in the
material collected from Bufo bufo (Bufonidae) in various parts of the country.
Morphological examination of O. duboisi and O. filiformis confirmed the charac-
ters proposed by Ben Slimane et al. (1995) for differentiation of these two species: the
cervical alae in O. duboisi are triangular on transverse sections and bear two small crests
on the dorsal side, whereas in O. filiformis the alae consist of two increased crests and
one smaller crest between them. Other morphological characters including metrical
ones are very similar in both species.
Use of some characters for differentiation of Oswaldocruzia is complicated by high
degree of their variability. We observed similar variations in shape of cephalic vesicle in
the specimens from separate hosts of O. duboisi: all three types of the vesicle were
observed in specimens from Pelophylax spp., M. alpestris and L. montadoni (tables 3,
4). On the other hand, all studied specimens from L. vulgaris possessed divided cephal-
ic vesicle (types II and III), though all other characters in those specimens were close
to the characters of specimens from other hosts (tables 3, 4).
e-7Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine
Unauthenticated
Download Date | 11/25/16 10:02 PM
e-8 R. S. Svitin, Y. I. Kuzmin
Ta b l e 3. Main measurements and types of cephalic vesicle of O. duboisi males from separate host species
Ò à á ëèö à 3. Îñíîâíûå ìåðíûå ïðèçíàêè è òèï ãîëîâíîé âåçèêóëû ó ñàìöîâ O. duboisi èç ðàçíûõ âèäîâ
õîçÿåâ
Ta b l e 4. Main measurements and types of cephalic vesicle of O. duboisi females from separate host species
Ò à á ëèö à 4. Îñíîâíûå ìåðíûå ïðèçíàêè è òèï ãîëîâíîé âåçèêóëû ó ñàìîê O. duboisi èç ðàçíûõ âèäîâ
õîçÿåâ
Characters
Pelophylax
spp.
M. alpestris L. montadoni L. vulgaris T. cristatus
N = 14 N = 4 N = 10 N = 13 N = 2
Length of body, mm 5.7—9.0 5.3—7.3 3,5—6.1 4.7—7.2 5.0—5.1
Width of body 110—190 130—180 90—170 120—180 450—530
Length of cephalic vesicle 58—83 78—83 53—68 53—78 70—78
Width of cephalic vesicle 40—48 38—43 35—45 28—75 28—45
Length of oesophagus 370—433 408—583 283—430 368—433 375—380
Length of oesophagus, in % of body
length
4.72—7.11 7.11—8.00 4.76—9.89 5.97—8.28 7.41—7.58
Width of anterior end of oespohagus 23—29 25—25 23—25 23—28 25—25
Width of oespohagus at midlength 20—28 23—28 23—25 23—25 23—23
Width of oesophageal bulb 45—63 53—58 38—58 43—58 45—48
Distance to nerve ring from anterior end
of oesophagus
150—200 165—268 115—205 143—203 205—208
Distance to nerve ring, in % of oesoph-
agus length
37.65—51.61 39.2—45.9 37.8—57.8 35.4—47.9 54.6—54.7
Distance to excretory pore from anterior
end of oesophagus
223—333 210—328 233—303 198—348 248—248
Distance to excretory pore, in % of
esophagus length
56.8—83.0 51.5—65.1 56.4—86.8 49.1—87.4 65.1—66.0
Length of tail 95—133 118—168 98—130 103—155 103—115
Length of spicules 160—258 155—180 158—205 165—203 158—163
Length of spicules in % of body length 2.50—4.59 2.13—3.0 3.32—4.50 2.82—3.50 3.12—3.22
Type of cephalic vesicle I, II, III II, III I, II, III II, III II
Characters
Pelophylax spp. M. alpestris L. montadoni L. vulgaris
N = 13 N = 12 N = 7 N = 11
Length of body, mm 6.3—13.6 6.8—8.8 8.7—11.4 2.3—13.6
Width of body at midlength 100—250 120—180 150—190 140—250
Length of cephalic vesicle 50—95 63—95 60—78 50—80
Width of cephalic vesicle 38—50 43—48 40—50 38—50
Length of oesophagus 375—508 403—483 423—508 378—490
Length of oesophagus, in % of body length 3.47—6.17 5.50—5.90 4.41—5.70 3.6—5.7
Width of anterior end of oespohagus 25—33 25—30 25—33 25—32
Width of oesophagus at midlength 23—33 23—28 25—28 25—33
Width of oesophageal bulb 43—65 50—65 50—63 43—65
Distance to nerve ring from anterior end of
oesophagus
160—245 163—238 163—238 160—245
Distance to nerve ring, in % of oesophagus
length
37.4—51.2 38.3—50.3 37.4—47.0 38.5—51.2
Distance to excretory pore from anterior end
of oesophagus
190—433 203—303 235—325 190—338
Distance to excretory pore, in % of oesoph-
agus length
46.6—86.1 46.6—69.5 55.3—68.8 49.0—71.4
Distance from anterior end to vulva, mm 2.8—8.4 3.9—5.8 5.5—7.3 2.8—8.4
Distance to vulva, in % of body length 43.9—68.6 51.8—67.2 62.1—65.2 43.9—51.1
Length of tail 148—273 175—223 178—248 148—255
Length of tail, in % of body length 1.67—2.95 2.21—2.75 1.87—2.57 1.67—7.42
Type of cephalic vesicle I, II, III I, II, III I, II, III II, III
Unauthenticated
Download Date | 11/25/16 10:02 PM
Metrical characters also varied significantly in the studied samples, except for the
width of oesophagus in its anterior and middle parts (tables 1, 2). Spicule length var-
ied within a wide range, from 155 up to 255 μm; this variation corresponds with range
of spicule length given in the first description of the species – 155—250 μm (Ben
Slimane et al., 1993). We found no correlation between the variations of metrical char-
acters in O. duboisi and its host species or geographic locality (see tables 3 and 4). The
only exception was observed in 5 males with comparatively larger spicules, all collect-
ed from 2 specimens of P. ridibunda in Crimea. This sample may represent a separat-
ed lineage within the species O. duboisi, or may belong to a separate cryptic species.
The authors wish to express their sincere thanks to Dr. Marie-Claude Durette-Desset (Museum
National d’Histoire Naturelle, Paris, France) for the reprints of her publications granted to the authors.
Anderson N. C. Nematode Parasites of Vertebrates: their Development and Transmission. 2nd Edition. –
London : CAB Publishing, 2000. – 672 p.
Baker M. R. On three Oswaldocruzia spp. (Trichostrongyloidea: Molineidae) in Amphibians from Africa //
Canadian Journal of Zoology. – 1981. – N 59 (2). – P. 246—251.
Ben Slimane B., Durette-Desset M. C., Chabaud A. G. Oswaldocruzia (Trichostrongyloidea) parasites
d’Amphibiens des Collections du Museum de Paris // Ann. Parasitol. Hum. Comp. – 1993. – N 2. –
P. 88—100.
Ben Slimane B., Lluch J., Durette-Desset M. C. Two new species of the genus Oswaldocruzia Travassos, 1917
(Nematoda: Trichostrongylina: Molineoidea) parasitizing Spanish amphibians // Research and Reviews
in Parasitology. – 1995. – N 55 (4). – P. 209—215.
Brooks D. R., León-Règanon V., McLennan D. A., Zelmer D. Ecological fitting as a determinant of the com-
munity structure of platyhelminth parasites of anurans // Ecology. – 2006. – Supplement. –
P. 76—85.
Durette-Desset M. C. Trichostrongyloid nematodes and their vertebrate hosts: reconstruction of the phyloge-
ny of parasitic group // Advances in Parasitology. – 1985. – N 24. – P. 239—306.
Durette-Desset M. C., Batcharov G., Ben Slimane B., Chabaud A. G. Some Oswaldocruzia (Nematoda:
Trichostrongyloidea) parasites of Amphibia in Bulgaria. Redescription of Oswaldocruzia bialata (Molin,
1860) // Helmintologia. – 1993. – N 30. – P. 99—104.
Durette-Desset M. C., Chabaud A. G. Nouvel essai de classification des Nematodes Trichostrongyloidea //
Annales de Parasitologie Humaine et Comparee. – 1981. – N 56. – P. 297—312.
Galli P., Grosa G., Gentilli A., Santagostino M. New geographical records of parasitic nematodes from Bufo
bufo in Italy // Parassitologia. – 2001. – N 43. – P. 147—149.
Lal B. M. A new Amphibian Trichostrongylid // Current Science. – 1944. – N 13. Ð. 104—105
Novokhatskaya O. V. Parasitofauna of fish of eutrophied lakes (on example of Syamozero): Abstract for the-
sis of dissertation for candidate of biological sciences. – St.-Petersburg, 2008. – 31 p. – Russian :
Íîâîõàöêàÿ Î. Â. Ïàðàçèòîôàóíà ðûá ýâòðîôèðóåìûõ îç¸ð (íà ïðèìåðå Ñÿìîçåðà) : Àâòîðåô.
äèñ. ... êàíä. áèîë. íàóê. – ÑÏá., 2008. – 31 ñ.
Odnokurtsev V. A., Sedalishchev V. T. Helminthic fauna of Siberian wood frog (Rana amurensis Boulenger,
1886) from Yacutia, its sex-age and seasonal variability // Povolzhskiy Journal of Ecology. – 2008. –
N 2. – P. 112—119.
Pisanets E. M. Amphibians of Ukraine (Guide to Amphibians of Ukraine and Adjacent Territories). – Kyiv :
Zoological Museum of NSNM NAS of Ukraine, 2007. – 312 p. – Russian : Ïèñàíåö Å. Ì. Àìôèáèè
Óêðàèíû (ñïðàâî÷íèê-îïðåäåëèòåëü çåìíîâîäíûõ Óêðàèíû è ñîïðåäåëüíûõ òåððèòîðèé).
Ryzhikov K. M., Sharpilo V. P., Shevchenko N. N. Helmints of the amphibians of the fauna of the USSR. –
Moskow : Nauka, 1980. – 276 p. – Russian : Ðûæèêîâ Ê. Ì., Øàðïèëî Â. Ï., Øåâ÷åíêî
Í. Í. Ãåëüìèíòû àìôèáèé ôàóíû ÑÑÑÐ.
Skrjabin K. I., Shichobalova N. P., Shultz R. S. Trichostrongylids of animals and man. Fundamentals of
nematodology. Vol. 3. – Moscow : Publishing of Academy of Science of the USSR, 1954. – 683 p. –
Russian: Ñêðÿáèí Ê. È., Øèõîáàëîâà Í. Ï., Øóëüö Ð. Ñ. Òðèõîñòðîíãèëèäû æèâîòíûõ è ÷åëîâå-
êà. Îñíîâû íåìàòîäîëîãèè. Ò. 3.
Yildrimhan S. H. Seritli Semender (Triturus vittatus (Jenyns, 1835)) ve Purtuklu Semender (Triturus kareli-
ni (Strauch, 1870)) ‘lerin Helmint Faunasi Uzerine Bir On Calisma // Turkiye Parazitoloji Dergisi. –
2008. – 32, N 2. – P. 158—160.
Schotthoefer A. M., Bolek M. G., Cole R. A., Beasley V. R. Parasites of the Mink Frog (Rana eptentrionalis)
from Minessota, U. S. A. // Comparative Parasitology. – 2009. –76 (2). – P. 240—246.
Shcherbak N. N., Shcherban M. I. Amphibians and Reptiles of Ukrainian Carpathians. – Kyiv : Naukova
Dumka, 1980. – 266 p. – Russian : Ùåðáàê Í. Í., Ùåðáàíü Ì. È. Çåìíîâîäíûå è ïðåñìûêàþ-
ùèåñÿ Óêðàèíñêèõ Êàðïàò.
e-9Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine
Unauthenticated
Download Date | 11/25/16 10:02 PM
<<
/ASCII85EncodePages false
/AllowTransparency false
/AutoPositionEPSFiles true
/AutoRotatePages /None
/Binding /Left
/CalGrayProfile (None)
/CalRGBProfile (sRGB IEC61966-2.1)
/CalCMYKProfile (ISO Coated)
/sRGBProfile (sRGB IEC61966-2.1)
/CannotEmbedFontPolicy /Error
/CompatibilityLevel 1.3
/CompressObjects /Off
/CompressPages true
/ConvertImagesToIndexed true
/PassThroughJPEGImages true
/CreateJDFFile false
/CreateJobTicket false
/DefaultRenderingIntent /Perceptual
/DetectBlends true
/DetectCurves 0.1000
/ColorConversionStrategy /sRGB
/DoThumbnails true
/EmbedAllFonts true
/EmbedOpenType false
/ParseICCProfilesInComments true
/EmbedJobOptions true
/DSCReportingLevel 0
/EmitDSCWarnings false
/EndPage -1
/ImageMemory 524288
/LockDistillerParams true
/MaxSubsetPct 100
/Optimize true
/OPM 1
/ParseDSCComments true
/ParseDSCCommentsForDocInfo true
/PreserveCopyPage true
/PreserveDICMYKValues true
/PreserveEPSInfo true
/PreserveFlatness true
/PreserveHalftoneInfo false
/PreserveOPIComments false
/PreserveOverprintSettings true
/StartPage 1
/SubsetFonts false
/TransferFunctionInfo /Apply
/UCRandBGInfo /Preserve
/UsePrologue false
/ColorSettingsFile ()
/AlwaysEmbed [ true
]
/NeverEmbed [ true
]
/AntiAliasColorImages false
/CropColorImages true
/ColorImageMinResolution 150
/ColorImageMinResolutionPolicy /OK
/DownsampleColorImages true
/ColorImageDownsampleType /Bicubic
/ColorImageResolution 150
/ColorImageDepth -1
/ColorImageMinDownsampleDepth 1
/ColorImageDownsampleThreshold 2.00000
/EncodeColorImages true
/ColorImageFilter /DCTEncode
/AutoFilterColorImages false
/ColorImageAutoFilterStrategy /JPEG
/ColorACSImageDict <<
/QFactor 0.76
/HSamples [2 1 1 2] /VSamples [2 1 1 2]
>>
/ColorImageDict <<
/QFactor 0.76
/HSamples [2 1 1 2] /VSamples [2 1 1 2]
>>
/JPEG2000ColorACSImageDict <<
/TileWidth 256
/TileHeight 256
/Quality 30
>>
/JPEG2000ColorImageDict <<
/TileWidth 256
/TileHeight 256
/Quality 30
>>
/AntiAliasGrayImages false
/CropGrayImages true
/GrayImageMinResolution 150
/GrayImageMinResolutionPolicy /OK
/DownsampleGrayImages true
/GrayImageDownsampleType /Bicubic
/GrayImageResolution 150
/GrayImageDepth -1
/GrayImageMinDownsampleDepth 2
/GrayImageDownsampleThreshold 2.00000
/EncodeGrayImages true
/GrayImageFilter /DCTEncode
/AutoFilterGrayImages true
/GrayImageAutoFilterStrategy /JPEG
/GrayACSImageDict <<
/QFactor 0.76
/HSamples [2 1 1 2] /VSamples [2 1 1 2]
>>
/GrayImageDict <<
/QFactor 0.15
/HSamples [1 1 1 1] /VSamples [1 1 1 1]
>>
/JPEG2000GrayACSImageDict <<
/TileWidth 256
/TileHeight 256
/Quality 30
>>
/JPEG2000GrayImageDict <<
/TileWidth 256
/TileHeight 256
/Quality 30
>>
/AntiAliasMonoImages false
/CropMonoImages true
/MonoImageMinResolution 1200
/MonoImageMinResolutionPolicy /OK
/DownsampleMonoImages true
/MonoImageDownsampleType /Bicubic
/MonoImageResolution 600
/MonoImageDepth -1
/MonoImageDownsampleThreshold 1.50000
/EncodeMonoImages true
/MonoImageFilter /CCITTFaxEncode
/MonoImageDict <<
/K -1
>>
/AllowPSXObjects false
/CheckCompliance [
/None
]
/PDFX1aCheck false
/PDFX3Check false
/PDFXCompliantPDFOnly false
/PDFXNoTrimBoxError true
/PDFXTrimBoxToMediaBoxOffset [
0.00000
0.00000
0.00000
0.00000
]
/PDFXSetBleedBoxToMediaBox true
/PDFXBleedBoxToTrimBoxOffset [
0.00000
0.00000
0.00000
0.00000
]
/PDFXOutputIntentProfile (None)
/PDFXOutputConditionIdentifier ()
/PDFXOutputCondition ()
/PDFXRegistryName (http://www.color.org?)
/PDFXTrapped /False
/SyntheticBoldness 1.000000
/Description <<
/ENU (Versita Adobe Distiller Settings for Adobe Acrobat v6)
/POL (Versita Adobe Distiller Settings for Adobe Acrobat v6)
>>
>> setdistillerparams
<<
/HWResolution [2400 2400]
/PageSize [2834.646 2834.646]
>> setpagedevice
|
| id | nasplib_isofts_kiev_ua-123456789-109372 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 0084-5604 |
| language | English |
| last_indexed | 2025-12-07T16:35:00Z |
| publishDate | 2012 |
| publisher | Інститут зоології ім. І.І. Шмальгаузена НАН України |
| record_format | dspace |
| spelling | Svitin, R.S. Kuzmin, Y.I. 2016-11-27T17:13:39Z 2016-11-27T17:13:39Z 2012 Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine / R.S. Svitin, Y.I. Kuzmin // Вестник зоологии. — 2012. — Т. 46, № 3. — С. 195-203. — Бібліогр.: 18 назв. — англ. 0084-5604 https://nasplib.isofts.kiev.ua/handle/123456789/109372 595.132 DOI 10.2478/v10058-012-0017-x Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud, 1993 previously known from France and Bulgaria is reported from Ukraine for the first time. The species was found in the material from 8 amphibian host species, of which Lissotriton montadoni, Triturus cristatus, Mesotriton alpestris, Pelophylax ridibunda, P. lessonae, and Hyla arborea are new host records. Newts (Salamandridae) and green frogs (Pelophylax) are considered to be typical hosts for O. duboisi. Illustrated morphological redescription of O. duboisi based on 141 specimens from various hosts is presented. Вид Oswaldocruzia duboisi Ben Slimane, Durette-Desset et Chabaud, 1993, ранее известный из Франции и Болгарии, впервые обнаружен в Украине. Вид найден в материале от 8 видов амфибий, из которых Lissotriton montadoni, Triturus cristatus, Mesotriton alpestris, Pelophylax ridibunda, P. lessonae и Hyla arborea являются новыми хозяевами O. duboisi. Предполагается, что основными хозяевами O. duboisi являются тритоны (Salamandridae) и зеленые лягушки (род Pelophylax). Представлено иллюстрированное описание O. duboisi по 141 изученному экземпляру от разных видов хозяев. The authors wish to express their sincere thanks to Dr. Marie-Claude Durette-Desset (Museum National d’Histoire Naturelle, Paris, France) for the reprints of her publications granted to the authors. en Інститут зоології ім. І.І. Шмальгаузена НАН України Вестник зоологии Фауна и систематика Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine Oswaldocruzia duboisi (Nematoda, Molineidae): морфология, хозяева и распространение в Украине Article published earlier |
| spellingShingle | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine Svitin, R.S. Kuzmin, Y.I. Фауна и систематика |
| title | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine |
| title_alt | Oswaldocruzia duboisi (Nematoda, Molineidae): морфология, хозяева и распространение в Украине |
| title_full | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine |
| title_fullStr | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine |
| title_full_unstemmed | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine |
| title_short | Oswaldocruzia duboisi (Nematoda, Molineidae): Morphology, Hosts and Distribution in Ukraine |
| title_sort | oswaldocruzia duboisi (nematoda, molineidae): morphology, hosts and distribution in ukraine |
| topic | Фауна и систематика |
| topic_facet | Фауна и систематика |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/109372 |
| work_keys_str_mv | AT svitinrs oswaldocruziaduboisinematodamolineidaemorphologyhostsanddistributioninukraine AT kuzminyi oswaldocruziaduboisinematodamolineidaemorphologyhostsanddistributioninukraine AT svitinrs oswaldocruziaduboisinematodamolineidaemorfologiâhozâevairasprostranenievukraine AT kuzminyi oswaldocruziaduboisinematodamolineidaemorfologiâhozâevairasprostranenievukraine |