Plasma sterilizer with ultrasonic cavitation
A sterilizer consists of ozone generator based on a barrier glow discharge with the flat electrodes covered with dielectric with a high-voltage pulsed power supply of up to 250 W [1]. The sterilization camera is equipped with ultrasonic source with the power of 100 W. The experiments on the inactiva...
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| Date: | 2007 |
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Національний науковий центр «Харківський фізико-технічний інститут» НАН України
2007
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| Cite this: | Plasma sterilizer with ultrasonic cavitation / V.V. Krasnyj, A.V. Klosovskij, T.A. Panasko, O.M. Shvets, O.T. Semenova, V.S. Taran, V.I. Tereshin // Вопросы атомной науки и техники. — 2007. — № 1. — С. 188-190. — Бібліогр.: 3 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859735675398520832 |
|---|---|
| author | Krasnyj, V.V. Klosovskij, A.V. Panasko, T.A. Shvets, O.M. Semenova, O.T. Taran, V.S. Tereshin, V.I. |
| author_facet | Krasnyj, V.V. Klosovskij, A.V. Panasko, T.A. Shvets, O.M. Semenova, O.T. Taran, V.S. Tereshin, V.I. |
| citation_txt | Plasma sterilizer with ultrasonic cavitation / V.V. Krasnyj, A.V. Klosovskij, T.A. Panasko, O.M. Shvets, O.T. Semenova, V.S. Taran, V.I. Tereshin // Вопросы атомной науки и техники. — 2007. — № 1. — С. 188-190. — Бібліогр.: 3 назв. — англ. |
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| container_title | Вопросы атомной науки и техники |
| description | A sterilizer consists of ozone generator based on a barrier glow discharge with the flat electrodes covered with dielectric with a high-voltage pulsed power supply of up to 250 W [1]. The sterilization camera is equipped with ultrasonic source with the power of 100 W. The experiments on the inactivation of bacteria of the Bacillus Cereus type were carried out in the distilled water saturated by ozone. Ozone concentration in the aqueous solution was 6 mg/liter with ozone concentration at the output of ozone generator 30 mg/liter. The complete inactivation of spores took 15 min.
Розроблено озонний генератор на основі бар'єрного тліючого розряду з плоскими електродами, покритими діелектриком, з високовольтним імпульсним живленням, потужністю до 250 Вт [1]. Камера стерилізації оснащена ультразвуковим джерелом потужністю 100 Вт. Проведені дослідження інактивації спор Bacillus Cereus у дистильованій воді, насиченій озоном. Концентрація озону у водному розчині була 6 мг/л при концентрації озону на виході озонного генератора 30 мг/л. Повна інактивація спор відбувалась приблизно за 15 хв.
Разработан озонный генератор на основе барьерного тлеющего разряда с плоскими электродами, покрытыми диэлектриком, с высоковольтным импульсным питанием, мощностью до 250 Вт [1]. Стерилизационная камера оснащена ультразвуковым источником мощностью 100 Вт. Проведены исследования инактивации спор Bacillus Cereus в дистиллированной воде насыщенной озоном. Концентрация озона в водном растворе была 6 мг/л при концентрации озона на выходе озонного генератора 30 мг/л. Полная инактивация спор происходила за время порядка 15 мин.
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| first_indexed | 2025-12-01T14:42:55Z |
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188 Problems of Atomic Science and Technology. 2007, 1. Series: Plasma Physics (13), p. 188-190
PLASMA STERILIZER WITH ULTRASONIC CAVITATION
V.V. Krasnyj, A.V. Klosovskij, T.A. Panasko, O.M. Shvets, O.T. Semenova,
V.S. Taran, V.I. Tereshin
Institute of Plasma Physics, NSC “Kharkov Institute of Physics and Technology”,
Akademicheskaya Str.1, 61108 Kharkov, Ukraine, e-mail: vtaran@ ipp.kharkov.ua
A sterilizer consists of ozone generator based on a barrier glow discharge with the flat electrodes covered with
dielectric with a high-voltage pulsed power supply of up to 250 W [1]. The sterilization camera is equipped with
ultrasonic source with the power of 100 W. The experiments on the inactivation of bacteria of the Bacillus Cereus type
were carried out in the distilled water saturated by ozone. Ozone concentration in the aqueous solution was 6 mg/liter
with ozone concentration at the output of ozone generator 30 mg/liter. The complete inactivation of spores took 15 min.
PACS: 52.77.-j; 42.62.Be
1. INTRODUCTION
Plasma methods are widely applied in different
technological areas. For instance, in medicine they can be
utilized for sterilization of medical instruments. In
contrast to the traditional methods of sterilization (steam,
boiling, radiation, gaseous with the application of
ethylene and many others), plasma methods possess the
new properties, which make it possible to avoid the
temperature heating of the workable objects and toxic
ingredients. One of the important factors is lower power
consumption for sterilization procedures. Taking into
account several research works on sterilization in
microbiological media [2], it is possible to note that the
barrier surface discharge under atmospheric pressure used
for ozone generation turned to be a rather effective action
which can influence on the row of microorganisms. As
one of disadvantages it is possible to note the problems
with the insufficient cooling of the electrodes of reactor.
We should point out to industrial ozone sterilizer CO -01-
P6 (Russia) on the basis of lamellar reactor with the
vacuum working chamber. In ref. [3] the dependences of
the inactivation of spores Bacillus cereus in ozone by the
concentration of 3 mg/l with the relative humidity from
50% to 95% were investigated. In this case, the
effectiveness of inactivation grows with an increase in the
humidity. But even with 95% of humidity it does not
achieve complete destruction. In this case the cycle time
of sterilization is measured by hours. In the present work
experimental studies on the inactivation of spores Basillus
in the water with different concentration of ozone-air flow
are carried out. From the literature, the solubility of ozone
in the aqueous solutions depends on the specific
conditions of conducting the experiment: from the quality
of water, its temperature, concentration of the forced
ozone-air mixture. In our case the distilled water at room
temperature was used. The process of sterilization by
ozone is determined by its oxidizing properties and the
operation with contaminated medical instrument is rather
time-consuming. On the other hand, the methods of
ultrasonic cleaning and sterilization of tools also take
place. However, in order to obtain the effect of
sterilization it is necessary to add into water chemical
active additives. In our case, we attempted to combine
generation of ozone in the aqueous medium with the
ultrasonic cavitations. Thus, it is possible to carry out
rough low-temperature cleaning of the surface of medical
instrument with the ozone sterilization.
2. EXPERIMENTAL
The functional diagram of sterilizer is presented in Fig.1.
Airflow from the compressor was cleaned from the
moisture through the filter and get into the ozone reactor.
Ozone-air mixture enters mixer (1) where ozone mixes
with the water entering from sterilization camera (4) with
the help of the specially developed anticorrosive
electromagnetic pump. The pressurized water mixed with
ozone enters sterilization camera. Worked out ozone is
moved away from the camera through destructor
(activated carbon) into the atmosphere. Sterilization
camera is supplied (5) with ultrasonic generator. The
reactor is constructed in the form of flat parallel plates
with the dielectric coatings with the pulsed power supply
[1].
Fig.1. The block diagram of the sterilizer: 1 - mixer,
2 - thermoelectric module, 3 - UV indicator ozone,
4 - sterilization camera, 5 - ultrasonic generator
The basic characteristics of the reactor are presented in
Fig.2.
0 ,1 1 1 0
0
1 0
2 0
3 0
4 0
1 ,0
2 ,0
3 ,0
4 ,0
Y , (g /h )
l , ( l /m in )
Y
C
0 6 63 7530 ,5
C
, (
m
g/
l)
0 ,2
Fig.2. Characteristics of ozone generator with
glass enamel dielectric: –ozone concentration,
Y – yield rate, l – air flow rate
When conducting an operating cycle in the
sterilization camera it is necessary to obtain concentration
in the water not less than 5 mg/l. Taking into account the
189
volume of sterilization camera, obtaining this
concentration is possible with the appropriate output
concentration from the output of reactor or with a
decrease of the water temperature in the camera. In Fig.3
the results of the solubility of ozone in the distilled water
with a volume of 1.5 liters depending on the time of
injection and constant (30 mg/l) concentration at the
output of reactor are represented.
0 6 12 18 24 30 36 42
0
1
2
3
4
5
6
7
8
9
, (
m
g/
l)
t, (min)
Ò3
Ò2
Ò1
Fig. 3. Solubility of ozone in the distilled water depending
on the time; water temperature: 1 - 13º C, 2 - 17.5º C,
3- 24 º C; concentration of ozone-air mixture is constant
(30 mg/l)
It is evident from the graph that the water temperature has
a great influence on ozone solubility and allows to reach
higher ozone concentration at lower temperatures. The
similar conclusions can be drawn from the analysis of
Fig.4, where it is shown that ozone concentration in the
water decreases under a slow increase of the water
temperature from 20 to 25o C.
0 10 20 30 40 50
0
5
10
15
20
25
0
5
4
3
2 C
, (
m
g/
l)
T
T,
(o C
)
t, (min)
C
1
Fig. 4. Dynamics of a change of ozone concentration
in the dependence on the water temperature,
= 0,07 atm, U = 300 V, = 0,3 l/min
The solubility of ozone in the water depends on the
output concentration of reactor (Fig. 5).
In the sterilizer the water-cooling took place in a
special mixer. A mixer is 0.5 l - vessel made of the
stainless steel, that consists of water sprayer and ozone
disperser. For the purpose of the stabilization of the
sterilization process and obtaining the necessary water
temperature, the mixer is equipped with thermoelectric
module. The result of the solubility of ozone in the water
with the periodic start of ultrasonic generator and the
work of thermoelectric module are shown in Fig. 6.
0 6 12 18 24 30
0
1
2
3
4
5
6
C
,(m
g/
l)
t,(min)
1
2
3
Fig. 5. Solubility of ozone in the distilled water
depending on time, with different concentration of ozone
at the output of the reactor: 1 – 30 mg/l, 2 – 25 mg/l,
3 – 20 mg/l
0 12 24 36 48 60 72 84 96 108 120
0
1
2
3
4
5
6
2
1
x
x
x
x xxx
C
, m
g/
l
t, min
21,60C
22,20C
22,60C 230C20,30C
210C
21,60C
22,20C
x
Fig.6. Solubility of ozone in the water with the periodic
start of ultrasonic generator. Sterilizations of leaves
3. RESULTS
The table presents the results of the inactivation of
spores Bacillus Cereus in the ozonized water with
different concentration of ozone at the output of reactor.
p/
p
(
3)
,m
g/
l
re
ac
to
r
C
(
3)
, m
g/
l
w
at
er
Ex
po
su
re
t,
m
in
°,
w
at
er
°,
ai
r
Method
R
es
ul
t
1 0 30 26 23 US, =220 Wt -
2 10 1.5 30 22 23 Ozone +US, =220 Wt -
3 11 2 60 20 23 Ozone +US, =220 Wt -
4 30 2.3 60 20 18 Ozone +US, =100 Wt +
5 30 6.2 180 18 18 Ozone +US, =15 Wt +
6 10 2 60 20 23 Ozone -
7 30 6 20 25 22 Ozone +
8 30 6 15 25 17,5 Ozone +
9 25 4.3 30 22 17,5 Ozone +
10 25 4.3 30 25 22 Ozone +
11 20 3.8 30 25 17,5 Ozone +
12 20 3.8 30 25 22 Ozone +
13 20 3.8 25 25 22 Ozone +
14 15 3 30 25 17,5 Ozone +
15 15 3 20 25 22 Ozone +
190
The effect of the complete destruction of spores is
observed with ozone concentration in the water not less
than 3 mg/l. Steady sterilization occurs with the
concentration not less than 5 mg/l.
4. CONCLUSIONS
The atmospheric pressure barrier glow discharge with
pulse power was adapted for ozone production. Ozone
reactor with the flat electrodes and the dielectric coatings
from the glass enamel had a value of the output ozone
concentration on the order of 30 mg/l with the air flow 0,5
l/min. Sterilization was achieved in the distilled water.
Ozone concentration in water is of the order of 6 mg/l.
The control of aqueous sterilization was conducted on the
cryptogamous Bacillus Cereus. Minimum time for the
complete inactivation of spores was 20 min. The process
of sterilization was combined with the ultrasonic
cavitation - cleaning. Influence on the process of cleaning
and sterilization US emitters with power from 10 to
250 W is investigated.
As a result of applying the developed sterilizer the
process of the sterilization of medical instrument will
obtain the following new preferred properties:
- combination of cleaning and sterilization of the tools;
- sterilization at a low temperature;
- exception of unnecessary chemical compounds;
- improvement in the quality of the sterilization of tool
due to the tight control and the stabilization of
technological process;
- lightening and convenience in the service personnel;
- reduction of prices of the sterilization process;
- realization of sterilizer on the basis of ultrasonic
washing.
REFERENCES
1. V.V.Krasnyj, A.V.Klosovski, A.S. Knysh,
O.M. Shvets, V.S. Taran, V.I. Tereshin. Influence of
duration and rate of pulse rise of the applied voltage on
ozone concentration in the barrier discharge// Conference
Proceedings of the International Conference “Plasma
2005” comb. with 3-rd GPPD and 5-th FPSTPS, Poland
v. 812, p. 345 – 348.
2. R. B. Gadri, J.R. Roth, T. C. Montie et al. Sterilization
and plasma processing of room temperatyre surfaces with
a one atmosphere uniform glow discharge plasma
(OAUGDP)// Surface and Coatings Technology. 2000,
v. 131, p. 538 – 542.
3. K. Ishizaki, N. Shinriki, H. Matsuyama // Journal of
Applied Bacteriology. 1986, v. 60, p. 67-72.
. , . , . , . , . , . , .
,
, , 250 [1].
100 .
Bacillus Cereus .
6 30 .
15 .
. , . , . , . , . , . , .
,
, , 250 [1].
100 . Bacillus
Cereus , .
6 30 .
15 .
|
| id | nasplib_isofts_kiev_ua-123456789-110532 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1562-6016 |
| language | English |
| last_indexed | 2025-12-01T14:42:55Z |
| publishDate | 2007 |
| publisher | Національний науковий центр «Харківський фізико-технічний інститут» НАН України |
| record_format | dspace |
| spelling | Krasnyj, V.V. Klosovskij, A.V. Panasko, T.A. Shvets, O.M. Semenova, O.T. Taran, V.S. Tereshin, V.I. 2017-01-04T18:26:29Z 2017-01-04T18:26:29Z 2007 Plasma sterilizer with ultrasonic cavitation / V.V. Krasnyj, A.V. Klosovskij, T.A. Panasko, O.M. Shvets, O.T. Semenova, V.S. Taran, V.I. Tereshin // Вопросы атомной науки и техники. — 2007. — № 1. — С. 188-190. — Бібліогр.: 3 назв. — англ. 1562-6016 PACS: 52.77.-j; 42.62.Be https://nasplib.isofts.kiev.ua/handle/123456789/110532 A sterilizer consists of ozone generator based on a barrier glow discharge with the flat electrodes covered with dielectric with a high-voltage pulsed power supply of up to 250 W [1]. The sterilization camera is equipped with ultrasonic source with the power of 100 W. The experiments on the inactivation of bacteria of the Bacillus Cereus type were carried out in the distilled water saturated by ozone. Ozone concentration in the aqueous solution was 6 mg/liter with ozone concentration at the output of ozone generator 30 mg/liter. The complete inactivation of spores took 15 min. Розроблено озонний генератор на основі бар'єрного тліючого розряду з плоскими електродами, покритими діелектриком, з високовольтним імпульсним живленням, потужністю до 250 Вт [1]. Камера стерилізації оснащена ультразвуковим джерелом потужністю 100 Вт. Проведені дослідження інактивації спор Bacillus Cereus у дистильованій воді, насиченій озоном. Концентрація озону у водному розчині була 6 мг/л при концентрації озону на виході озонного генератора 30 мг/л. Повна інактивація спор відбувалась приблизно за 15 хв. Разработан озонный генератор на основе барьерного тлеющего разряда с плоскими электродами, покрытыми диэлектриком, с высоковольтным импульсным питанием, мощностью до 250 Вт [1]. Стерилизационная камера оснащена ультразвуковым источником мощностью 100 Вт. Проведены исследования инактивации спор Bacillus Cereus в дистиллированной воде насыщенной озоном. Концентрация озона в водном растворе была 6 мг/л при концентрации озона на выходе озонного генератора 30 мг/л. Полная инактивация спор происходила за время порядка 15 мин. en Національний науковий центр «Харківський фізико-технічний інститут» НАН України Вопросы атомной науки и техники Low temperature plasma and plasma technologies Plasma sterilizer with ultrasonic cavitation Плазмовий стерилізатор з ультразвуковою кавітацією Плазменный стерилизатор с ультразвуковой кавитацией Article published earlier |
| spellingShingle | Plasma sterilizer with ultrasonic cavitation Krasnyj, V.V. Klosovskij, A.V. Panasko, T.A. Shvets, O.M. Semenova, O.T. Taran, V.S. Tereshin, V.I. Low temperature plasma and plasma technologies |
| title | Plasma sterilizer with ultrasonic cavitation |
| title_alt | Плазмовий стерилізатор з ультразвуковою кавітацією Плазменный стерилизатор с ультразвуковой кавитацией |
| title_full | Plasma sterilizer with ultrasonic cavitation |
| title_fullStr | Plasma sterilizer with ultrasonic cavitation |
| title_full_unstemmed | Plasma sterilizer with ultrasonic cavitation |
| title_short | Plasma sterilizer with ultrasonic cavitation |
| title_sort | plasma sterilizer with ultrasonic cavitation |
| topic | Low temperature plasma and plasma technologies |
| topic_facet | Low temperature plasma and plasma technologies |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/110532 |
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