Biochemical passivation of metal surfaces for sensor application: reactive annealing of polycrystalline gold films in hydrogen sulfide atmosphere

• Veröffentlicht in: Semiconductor Physics Quantum Electronics & Optoelectronics
• Datum: 2000
• Hauptverfasser: Snopok, B. A., Kostyukevych, K.V., Beketov, G.V., Zinio, S.A., Shirshov, Yu.M., Venger, E.F.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України 2000
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/120238
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Biochemical passivation of metal surfaces for sensor application: reactive annealing of polycrystalline gold films in hydrogen sulfide atmosphere / B.A. Snopok, K.V. Kostyukevych, G.V. Beketov, S.A. Zinio, Yu.M. Shirshov, E.F. Venger // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2000. — Т. 3, № 1. — С. 59-68. — Бібліогр.: 50 назв. — англ.

The formation of AuxSy interfacial layer by reactive annealing of gold films in H₂S atmosphere is investigated. This seems to be a technologically favorable technique for the large-scale and low-cost fabrication of nondestructive immobilization support for biological molecules. Formation of phases with different chemical functionality and surface topography as a function of reaction time was studied using Atomic Force Microscopy (AFM), Surface Plasmon Resonance (SPR) measurements and biomolecular interaction analysis (trypsin - Soybean Trypsin Inhibitor (STI) reaction). The results obtained confirm the classical two-step model for the sulfide phase formation during reactive annealing. This includes an intermediate formation of a dispersed phase of sulfur followed by its reconstruction to a close-packed sulfide layer. Adsorption of proteins onto a certain sulfide layer passes with retention of a native state of adsorbed molecules. The proposed strategy for formation of biochemical structures (gold/sulfide/proteins) on the surface of physical transducers opens a new way for design and development of novel artificial smart sensing systems. They not only maintain optimal functioning of bioreceptors but also are responsive to their environment.