Holographic interferometry as a method to study conformational changes in macromolecules
This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (Δn) influenced by the sample volume expansion due to temperature...
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| Veröffentlicht in: | Semiconductor Physics Quantum Electronics & Optoelectronics |
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| Datum: | 2002 |
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | Englisch |
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Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України
2002
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/121293 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Holographic interferometry as a method to study conformational changes in macromolecules / Y. Barabash, V. Kharkyanen, M. Zabolotny, N. Sokolov // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2002. — Т. 5, № 3. — С. 307-311. — Бібліогр.: 5 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862733529116311552 |
|---|---|
| author | Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. |
| author_facet | Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. |
| citation_txt | Holographic interferometry as a method to study conformational changes in macromolecules / Y. Barabash, V. Kharkyanen, M. Zabolotny, N. Sokolov // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2002. — Т. 5, № 3. — С. 307-311. — Бібліогр.: 5 назв. — англ. |
| collection | DSpace DC |
| container_title | Semiconductor Physics Quantum Electronics & Optoelectronics |
| description | This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (Δn) influenced by the sample volume expansion due to temperature changes (ΔnT), light absorption (Δnabs) and changes in the volume of photoactive molecules (DnV). Using the scheme of temperature compensation we could reduce the task to the case ΔnT <<Δnabs, ΔnV. At RC concentrations close to 10¹⁵ cm-³, our experimental setup enabled to register the shift of an interference pattern up to 0.1 of the bandwidth. Our estimations for the case of a protein solution yields in relative changes in the molecular volume within 10-³...10-².
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| first_indexed | 2025-12-07T19:37:44Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-121293 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1560-8034 |
| language | English |
| last_indexed | 2025-12-07T19:37:44Z |
| publishDate | 2002 |
| publisher | Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України |
| record_format | dspace |
| spelling | Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. 2017-06-13T20:35:26Z 2017-06-13T20:35:26Z 2002 Holographic interferometry as a method to study conformational changes in macromolecules / Y. Barabash, V. Kharkyanen, M. Zabolotny, N. Sokolov // Semiconductor Physics Quantum Electronics & Optoelectronics. — 2002. — Т. 5, № 3. — С. 307-311. — Бібліогр.: 5 назв. — англ. 1560-8034 PACS: 42.40.K https://nasplib.isofts.kiev.ua/handle/123456789/121293 This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using the method of holographic interferometry. As a principal value, we studied changes of a solution refraction index (Δn) influenced by the sample volume expansion due to temperature changes (ΔnT), light absorption (Δnabs) and changes in the volume of photoactive molecules (DnV). Using the scheme of temperature compensation we could reduce the task to the case ΔnT <<Δnabs, ΔnV. At RC concentrations close to 10¹⁵ cm-³, our experimental setup enabled to register the shift of an interference pattern up to 0.1 of the bandwidth. Our estimations for the case of a protein solution yields in relative changes in the molecular volume within 10-³...10-². en Інститут фізики напівпровідників імені В.Є. Лашкарьова НАН України Semiconductor Physics Quantum Electronics & Optoelectronics Holographic interferometry as a method to study conformational changes in macromolecules Article published earlier |
| spellingShingle | Holographic interferometry as a method to study conformational changes in macromolecules Barabash, Y. Kharkyanen, V. Zabolotny, M. Sokolov, N. |
| title | Holographic interferometry as a method to study conformational changes in macromolecules |
| title_full | Holographic interferometry as a method to study conformational changes in macromolecules |
| title_fullStr | Holographic interferometry as a method to study conformational changes in macromolecules |
| title_full_unstemmed | Holographic interferometry as a method to study conformational changes in macromolecules |
| title_short | Holographic interferometry as a method to study conformational changes in macromolecules |
| title_sort | holographic interferometry as a method to study conformational changes in macromolecules |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/121293 |
| work_keys_str_mv | AT barabashy holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules AT kharkyanenv holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules AT zabolotnym holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules AT sokolovn holographicinterferometryasamethodtostudyconformationalchangesinmacromolecules |