Adsorption and chemisorption of galactose oxidase on silica surface
Experience accumulated over a number of years in developing of methods of immobilization of galactose oxidase from Fusarium graminearum on parent and modified silica matrices is analyzed. Sturdy adsorption of galactose oxidase on silica surface was observed, such heterogeneous specimens possessed by...
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| Published in: | Поверхность |
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| Date: | 2002 |
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| Format: | Article |
| Language: | English |
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Інститут хімії поверхні ім. О.О. Чуйка НАН України
2002
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/126362 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Adsorption and chemisorption of galactose oxidase on silica surface / L.V. Kondakova, V.V. Yanishpolskii, V.A., Tertykh // Поверхность. — 2002. — Вип. 7-8. — С. 150-157. — Бібліогр.: 20 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862634993672519680 |
|---|---|
| author | Kondakova, L.V. Yanishpolskii, V.V. Tertykh, V.A. |
| author_facet | Kondakova, L.V. Yanishpolskii, V.V. Tertykh, V.A. |
| citation_txt | Adsorption and chemisorption of galactose oxidase on silica surface / L.V. Kondakova, V.V. Yanishpolskii, V.A., Tertykh // Поверхность. — 2002. — Вип. 7-8. — С. 150-157. — Бібліогр.: 20 назв. — англ. |
| collection | DSpace DC |
| container_title | Поверхность |
| description | Experience accumulated over a number of years in developing of methods of immobilization of galactose oxidase from Fusarium graminearum on parent and modified silica matrices is analyzed. Sturdy adsorption of galactose oxidase on silica surface was observed, such heterogeneous specimens possessed by enhanced biocatalyst stability and activity as compared with enzyme solutions. Covalent immobilization of galactose oxidase was carried out on the amine-containing silicas activated by 2,4-tolylene diisocyanate and cyanuric chloride. It was also shown that in the presence of the substrate (galactose) enzyme chemisorption takes place on the surface on aminecontaining silica matrices. Immobilized preparations were successfully applied for analytical determination of galactose-containing carbohydrates (galactose, lactose, raffinose) in complex mixtures.
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| first_indexed | 2025-11-30T16:58:17Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-126362 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | XXXX-0106 |
| language | English |
| last_indexed | 2025-11-30T16:58:17Z |
| publishDate | 2002 |
| publisher | Інститут хімії поверхні ім. О.О. Чуйка НАН України |
| record_format | dspace |
| spelling | Kondakova, L.V. Yanishpolskii, V.V. Tertykh, V.A. 2017-11-20T18:46:16Z 2017-11-20T18:46:16Z 2002 Adsorption and chemisorption of galactose oxidase on silica surface / L.V. Kondakova, V.V. Yanishpolskii, V.A., Tertykh // Поверхность. — 2002. — Вип. 7-8. — С. 150-157. — Бібліогр.: 20 назв. — англ. XXXX-0106 https://nasplib.isofts.kiev.ua/handle/123456789/126362 Experience accumulated over a number of years in developing of methods of immobilization of galactose oxidase from Fusarium graminearum on parent and modified silica matrices is analyzed. Sturdy adsorption of galactose oxidase on silica surface was observed, such heterogeneous specimens possessed by enhanced biocatalyst stability and activity as compared with enzyme solutions. Covalent immobilization of galactose oxidase was carried out on the amine-containing silicas activated by 2,4-tolylene diisocyanate and cyanuric chloride. It was also shown that in the presence of the substrate (galactose) enzyme chemisorption takes place on the surface on aminecontaining silica matrices. Immobilized preparations were successfully applied for analytical determination of galactose-containing carbohydrates (galactose, lactose, raffinose) in complex mixtures. The authors render thanks to T.T. Buglova and O.V. Skorobogat’ko from D.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of Ukraine for submitting of the samples of galactose oxidase from Fusarium graminearum IMV-1060. en Інститут хімії поверхні ім. О.О. Чуйка НАН України Поверхность Surface chemistry of silica and related sorbents Adsorption and chemisorption of galactose oxidase on silica surface Article published earlier |
| spellingShingle | Adsorption and chemisorption of galactose oxidase on silica surface Kondakova, L.V. Yanishpolskii, V.V. Tertykh, V.A. Surface chemistry of silica and related sorbents |
| title | Adsorption and chemisorption of galactose oxidase on silica surface |
| title_full | Adsorption and chemisorption of galactose oxidase on silica surface |
| title_fullStr | Adsorption and chemisorption of galactose oxidase on silica surface |
| title_full_unstemmed | Adsorption and chemisorption of galactose oxidase on silica surface |
| title_short | Adsorption and chemisorption of galactose oxidase on silica surface |
| title_sort | adsorption and chemisorption of galactose oxidase on silica surface |
| topic | Surface chemistry of silica and related sorbents |
| topic_facet | Surface chemistry of silica and related sorbents |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/126362 |
| work_keys_str_mv | AT kondakovalv adsorptionandchemisorptionofgalactoseoxidaseonsilicasurface AT yanishpolskiivv adsorptionandchemisorptionofgalactoseoxidaseonsilicasurface AT tertykhva adsorptionandchemisorptionofgalactoseoxidaseonsilicasurface |