Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez
Copper-resistance of Antarctic microbial communities selected from different samples (sediments, plants etc.) was investigated at Cu²⁺ concentration 5, 25, 100 ppm. It was shown that all samples contain Cu²⁺-resistant microorganisms in large amount about 10⁴–10⁷ CFU/g of sample. There were found 15...
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| Date: | 2006 |
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Національний антарктичний науковий центр МОН України
2006
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| Cite this: | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez / G.O. Tashyreva, N.A. Matveewa, O.B. Tashyrev // Український антарктичний журнал. — 2006. — № 4-5. — С. 219-222. — Бібліогр.: 7 назв. — рос. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860251009702428672 |
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| author | Tashyreva, G.O. Matveewa, N.A. Tashyrev, O.B. |
| author_facet | Tashyreva, G.O. Matveewa, N.A. Tashyrev, O.B. |
| citation_txt | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez / G.O. Tashyreva, N.A. Matveewa, O.B. Tashyrev // Український антарктичний журнал. — 2006. — № 4-5. — С. 219-222. — Бібліогр.: 7 назв. — рос. |
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| description | Copper-resistance of Antarctic microbial communities selected from different samples (sediments, plants etc.) was investigated at Cu²⁺ concentration 5, 25, 100 ppm. It was shown that all samples contain Cu²⁺-resistant microorganisms in large amount about 10⁴–10⁷ CFU/g of sample. There were found 15 morphological types of bacteria, 3 types of actinomycetes, 5 types of fungi. For microorganisms of major part of samples (65%) 100 ppm Cu²⁺ did not show inhibition effect.
Було досліджено стійкість антарктичних мікробних угруповань, виділених з різних типів проб (осади, рослинність та ін.) до катіону Cu²⁺ у концентраційному діапазоні 5, 25 та 100 мг/л. Показано, що у вивчених пробах стійкі до міді мікроорганізми мають великий титр, який становить 10⁴–10⁷ колонійутворюючих одиниць/г проби. Розмаїття мідьрезистентних морфотипів для бактерій складає 15, актиноміцетів – 3, грибів – 5. Для мікроорганізмів більшості проб (65%)концентрація 100 мг/л Cu²⁺ не проявляла бактерицидної дії.
|
| first_indexed | 2025-12-07T18:43:12Z |
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G.O. Tashyreva: OCCURRENCE OF CU
2+
-MICROORGANISMS ON ANTARCTIC ISLAND GALINDES
УДК 579.695
OCCURRENCE OF CU
2+
-MICROORGANISMS ON ANTARCTIC ISLAND GALINDES
G.O. Tashyreva, N.A. Matveewa, O.B. Tashyrev
Institute of microbiology and virology of National Academy of Science of Ukraine,
Department of general and soil microbiology, Department of physyology of gas-oxydysing microorganisms, е mail:
tach@i.com.ua; cenoz@serv.imv.kiev.ua
Abstract. Copper-resistance of Antarctic microbial communities selected from different samples (sediments, plants etc.) was
investigated at Cu2+ concentration 5, 25, 100 ppm. It was shown that all samples contain Cu2+-resistant microorganisms in large
amount about 104–107 CFU/g of sample. There were found 15 morphological types of bacteria, 3 types of actinomycetes, 5 types of
fungi. For microorganisms of major part of samples (65%) 100 ppm Cu2+ did not show inhibition effect.
Key words: copper-resistance, Antarctic microbial communities.
Резюме. Було досліджено стійкість антарктичних мікробних угруповань, виділених з різних типів проб (осади, рослинність
та ін.) до катіону Cu2+ у концентраційному діапазоні 5, 25 та 100 мг/л. Показано, що у вивчених пробах стійкі до міді
мікроорганізми мають великий титр, який становить 104–107 колонійутворюючих одиниць/г проби. Розмаїття
мідьрезистентних морфотипів для бактерій складає 15, актиноміцетів – 3, грибів – 5. Для мікроорганізмів більшості проб
(65%)концентрація 100 мг/л Cu2+ не проявляла бактерицидної дії.
Introduction
Copper is one of the most toxic heavy metals for microorganisms. For such strains as Micrococcus sp.
Rhodospirillum photometrcum (Андреюк, 1992) 0.02 – 0.1 ppm Cu
2+
causes strong growth inhibition and for
Pseudomonas putida, (Mishra, 1999) Klebsiella sp. (Rudd, 1983) concentration of Cu
2+
above 0.5 ppm leads to
germinate effect. One of the exceptions is Cu-resistant Thiobacillus which use sulfur Cu-containing sulfide minerals as
the source of electrons (Панин, 1982, Rodricuez, 1985). Microbial communities are more resistant to influence of
copper then isolated strains because they realize a complex of protective mechanisms; Microorganisms of industrial
ecosystems are more resistant to Cu because of adaptation effects (Андреюк, 2001).
During the 8
th
seasonal expedition to the Ukrainian Antarctic Station ‘Academic Vernadsky’, which is situated on
Galindes island 63 samples (soils, stones, ice, water, plants etc.) were selected. As far as Antarctic microorganism exist
in extreme conditions (low temperature during major part of the year, absence of free water) we decided to characterize
their resistant to other extreme factors. In addition, it is known that rocks which form Antarctic island contain high
concentrations of heavy metals, including copper (Войткевич, 1977). The objective of our study was to investigate the
influence of Cu
2+
, one of the most toxic heavy metals,
on growth and homeostasis of Antarctic island (but not marine)
microbial communities, find Cu
2+
-resistant microorganisms. Information acquired in this way is important for
development of our still very limited knowledge of complex characteristic of Antarctic microbial communities.
Materials and methods
Getting samples
Samples were selected from the most typical for the Antarctic islands landscapes: at the top of the rocks, soils, that
contain high concentration of organic compounds, small lakes, lichens and moss fields. Samples were dried at 18–20
0
C
and packed in hermetic plastic bags.
Inoculate preparation
1 g of every dried sample was homogenized and suspended in 100 ml of sterile saline solution (0.9% NaCl) and
vigorously shaken for 5 min. Than sample solution was put in 20 sterile flacks (V=5 ml) and stored in refrigerator at –
25
0
C until inoculation. The necessity of storing Antarctic samples solution in refrigerator can be explained that they
contain a large number of psychrophyllic and psychrotolerant microorganisms. Storing inoculate at higher temperature
could cause succession in microbial communities that would lead to results which are not correct.
Preparation of Cu
2+
solution
УКРАЇНСЬКИЙ АНТАРКТИЧНИЙ
ЖУРНАЛ
УАЖ, № 4-5, 219-222, (2006)
mailto:tach@i.com.ua
G.O. Tashyreva: OCCURRENCE OF CU
2+
-MICROORGANISMS ON ANTARCTIC ISLAND GALINDES
For preparation of Cu
2+
solution we used chemical pure CuSO4 and suspended it in sterile distillate water in such
amount that final concentration of Cu
2+
was 1000 ppm. Solution was sterilized by boiling 30 min., than cooled to 25
о
С
and added to nutrient medium (meat broth with 2% of agar) to final concentration 5, 25 and 100 ppm of Cu
2+
.
Cultivation of microorganisms
Microbial growth was observed by determination of CFU (colony forming units) on solid medium meat broth with
2% of agar in Petri dishes at 15
0
C. Results were counted at 3, 5 and 7 days of cultivation. We used medium for
organoheterotrophic microorganisms because it can show the largest diversity of microorganisms that form microbial
community. Cu
2+
was used as selective factor to find it’s germinate concentration for Antarctic microorganisms.
Bacteriological counts
A number of Cu
2+
-resistant microorganisms was counted by formula:
M=N
1
n
K,
where M – number of colony forming units (CFU) per 1g of sample, N – number of CFU on Petri dishes, n – dilution of
sample, K – humidity coefficient of sample (as far as samples were dried, K = 1,02).
Results and discussions
It has been shown that all investigated samples contain a large diversity of Cu
2+
-resistant heterotrophic
microorganisms (Fig. 1, 2) (Fig. 1-4 see the color paste). There were found 15 morphological types of bacteria, 3 types
of actinomycetes, 5 types of fungi.
It was observed that there growing concentrations of Cu
2+
can have different impact on diversity of Antarctic
microorganisms. For 30% of samples growing concentrations of Cu
2+
caused decreasing of microbial diversity (Fig 3.).
For example for microorganisms selected from sample T19 growing at 5 ppm Cu
2+
microbial diversity 4 morphological
types while at 25 and 100 ppm Cu
2+
there is only one type. For 70% of samples different concentrations of Cu
2+
do not
influence on microbial diversity (Fig. 4). In sample T35 bacteria are represented with 1 morphological type at all
concentrations of Cu
2+
.
It was shown that 34 investigated Antarctic samples contain Cu
2+
-resistant microorganisms in large amount. It
doesn’t depend on the type of sample and is about 10
4
–10
7
CFU/g of sample (Table).
Table.
Bacteriological counts of Cu
2+
-resistant Antarctic microoganisms
Sample
№
Cu
2+
-resistant CFU/ g of sample,
Sample type Cu
2+
concentration in growth medium
5 ppm 25 ppm 100 ppm
Т19 5,6. 10
5
1,6
.
10
5
1
.
10
4
sludge
Т43 1,3
.
10
5
1,1
.
10
4
1,1
.
10
4
soil
Т44 1,1
.
10
5
8
.
10
4
1,1
.
10
4
soil
Т48 6,9
.
10
5
4,3
.
10
5
1,6
.
10
5
soil
Т50 4
.
10
4
2
.
10
4
absent sludge
Т51 7,3
.
10
5
5,4
.
10
5
2,9
.
10
5
sludge
Т52 1,8
.
10
5
2,1
.
10
5
absent sludge
Т53.1 3,5
.
10
5
3,4
.
10
5
1,1
.
10
4
sludge
Т56.1 6,0
.
10
5
3,0
.
10
5
2,1
.
10
4
lichens
Т60 3,8
.
10
5
3,7
.
10
5
2,1
.
10
4
moss
Т63 2,0
.
10
5
6,1
.
10
4
absent moss
Т20 2,1
.
10
7
2,1
.
10
7
2,1
.
10
7
soil
Т32 2,1
.
10
7
2,1
.
10
7
2,1
.
10
7
moss
Т33 2,1
.
10
7
2,1
.
10
7
2,1
.
10
7
sludge
Т54 2,1
.
10
7
2,1
.
10
7
2,1
.
10
7
lichens
Т57 2,1
.
10
7
2,1
.
10
7
2,1
.
10
7
lichens
Т36 7,1
.
10
4
1,6
.
10
5
2,2
.
10
7
soil
Т59 8,1
.
10
4
1,0
.
10
5
2,3
.
10
7
soil
There were found differences in germinate concentrations of Cu
2+
for microorganisms selected from different
samples. By criteria of germinate concentration of copper samples can be divided into 4 groups. For the first group
germinate concentration of Cu
2+
is 5 ppm. This group is represented with 1 sample (3% from total amount of samples).
Second group – germinate concentration 25 ppm (7% of samples); 3
rd
– germinate concentration 100 ppm (25%). The
fourth group was the biggest one represented with 23 samples (65%) – concentration 100 ppm Cu
2+
had no influence on
microbial growth. So, microorganisms selected from majority samples were resistant to 100 ppm Cu
2+
. Dispersion of
sensitive and resistant microorganisms is shown on Fig.5.
G.O. Tashyreva: OCCURRENCE OF CU
2+
-MICROORGANISMS ON ANTARCTIC ISLAND GALINDES
65
%
3%
7%
25
%
5%
5 25 100 > 100 Cu
2+
, pm 5 25 100 > 100 Cu
2+
, pm
Typical dispersion of Cu
2+
-
resistant microorganisms
N (%) from of samples that contain
Cu
2+
-resistant microorganims
N (%) from of samples that contain
Cu
2+
-resistant microorganisms
75
25
50
75
50
25
Fig.5A. Fig.5B. Dispersion of Cu
2+
-resistant
microorganisms selected from
Antarctic samples
Usually, number of samples that contain metal-resistant microorganisms decreases with increasing of metal
concentration (Fig. 5A). Unlike these, major part of Antarctic samples contain Cu
2+
-resistant microorganisms (67%),
while sensitive are in minor (in general – 23%) (Fig 5 B).
Conclusion
1. Copper-resistance is wide spread for heterotrophic microorganisms of Galindes island – Cu
2+
- resistant
microorganisms are selected from almost all samples.
2. Antarctic microbial communities show strong copper-resistance, wide diversity and large amount at Cu
2+
-
resistant CFU/g of sample concentration Cu
2+
concentration 5–100 ppm.
It can be concluded that such data perform scientific interest and need further investigation.
References
1. Mishra S.P., Chanahury G.R. Adsorption of Cu
2+
by charcoal, waste biomass Pseudomonas putida, Penicillum sp.//J.
Indian Inst. Sci.– 1999. – 79, № 4. – P. 303–312.
2. Rodriguez M., Scotti M., Pichuantes S., Luksza M. Bacterial leaching of copper sulfide minerals by means of
Thiobacillus ferrooxidans// Rev. of latinoamer. microbiol. – 1985 – 27, № 3. – P. 219–224.
3. Rudd Th., Sterritt R.M., Lester J.N. Mass balance of heavy metal uptake by encapsulated cultures of Klebsiella
aerogenes// Microbial Ecol., 1983. V.9, № 3. – P. 261–272.
4. Андреюк Е.И., Валагурова Е.В. Основы экологии почвенных микроорганизмов// Киев:, „Наукова думка”. –
1992. 223 с.
5. Андреюк К.І, Іутинська Г.О., Антипчук А.Ф. Функціонування муробних ценозів в умовах антропогенного
навантаження.// К: – Обереги, 2001. – 240 с.
6. Войткевич Г.В., Мирошников А.Е., Поваренных С.А., Прохоров В.Г. Краткий справочник по геохимии.// М:.–
Недра, 1977. – 184 с.
7. Панин В.В., Каравайко Г.И., Полькин С.И. Механизм и кинетика бактериального окисления сульфидных
минералов.// Материалы международного научного семинара «Современные аспекты микробиологической
гидрометаллургии». – Москва. – 1985. – 435 с.
|
| id | nasplib_isofts_kiev_ua-123456789-128267 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1727-7485 |
| language | Russian |
| last_indexed | 2025-12-07T18:43:12Z |
| publishDate | 2006 |
| publisher | Національний антарктичний науковий центр МОН України |
| record_format | dspace |
| spelling | Tashyreva, G.O. Matveewa, N.A. Tashyrev, O.B. 2018-01-07T15:50:26Z 2018-01-07T15:50:26Z 2006 Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez / G.O. Tashyreva, N.A. Matveewa, O.B. Tashyrev // Український антарктичний журнал. — 2006. — № 4-5. — С. 219-222. — Бібліогр.: 7 назв. — рос. 1727-7485 https://nasplib.isofts.kiev.ua/handle/123456789/128267 579.695 Copper-resistance of Antarctic microbial communities selected from different samples (sediments, plants etc.) was investigated at Cu²⁺ concentration 5, 25, 100 ppm. It was shown that all samples contain Cu²⁺-resistant microorganisms in large amount about 10⁴–10⁷ CFU/g of sample. There were found 15 morphological types of bacteria, 3 types of actinomycetes, 5 types of fungi. For microorganisms of major part of samples (65%) 100 ppm Cu²⁺ did not show inhibition effect. Було досліджено стійкість антарктичних мікробних угруповань, виділених з різних типів проб (осади, рослинність та ін.) до катіону Cu²⁺ у концентраційному діапазоні 5, 25 та 100 мг/л. Показано, що у вивчених пробах стійкі до міді мікроорганізми мають великий титр, який становить 10⁴–10⁷ колонійутворюючих одиниць/г проби. Розмаїття мідьрезистентних морфотипів для бактерій складає 15, актиноміцетів – 3, грибів – 5. Для мікроорганізмів більшості проб (65%)концентрація 100 мг/л Cu²⁺ не проявляла бактерицидної дії. ru Національний антарктичний науковий центр МОН України Український антарктичний журнал Біологічні дослідження Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez Article published earlier |
| spellingShingle | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez Tashyreva, G.O. Matveewa, N.A. Tashyrev, O.B. Біологічні дослідження |
| title | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez |
| title_full | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez |
| title_fullStr | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez |
| title_full_unstemmed | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez |
| title_short | Occurrence of Cu²⁺-resistant microorganisms on Antarctic island Galindez |
| title_sort | occurrence of cu²⁺-resistant microorganisms on antarctic island galindez |
| topic | Біологічні дослідження |
| topic_facet | Біологічні дослідження |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/128267 |
| work_keys_str_mv | AT tashyrevago occurrenceofcu2resistantmicroorganismsonantarcticislandgalindez AT matveewana occurrenceofcu2resistantmicroorganismsonantarcticislandgalindez AT tashyrevob occurrenceofcu2resistantmicroorganismsonantarcticislandgalindez |