Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants
The molecular analysis results of pigment-protein complexes of photosynthetic membranes of Deschampsia antarctica plants, one of the native representatives of the Antarctic region are presented. Bioinformatics searching of genome products of Deschampsia antarctica in available protein databeses rela...
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Національний антарктичний науковий центр МОН України
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nasplib_isofts_kiev_ua-123456789-1284162025-02-23T17:50:04Z Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants Taran, N.Yu. Ozheredova, I.P. Storozhenko, V.О. Svetlova, N.B. Topchiy, N.М. Біологічні дослідження The molecular analysis results of pigment-protein complexes of photosynthetic membranes of Deschampsia antarctica plants, one of the native representatives of the Antarctic region are presented. Bioinformatics searching of genome products of Deschampsia antarctica in available protein databeses related to the structure and function of plastids was carried. The searching of Arabidopsis thaliana and Oryza sativa japonica homolog search submitted in free databases is also performed. The results of comparative analysis of primary sequences of the known and potential products are presented. Приведены результаты молекулярного анализа пигмент-белковых комплексов и рассмотрены особенности липидного состава фотосинтетических мембран Deschampsia antarctica – одного из представителей флоры Антарктики. Осуществлен биоинформационный поиск в доступных базах данных продуктов генома Deschampsia antarctica, которые структурно и функционально связаны с пластидами. Также осуществлен поиск их гомологов в протеомах Arabidopsis thaliana и Oryza sativa japonica. Приведены результаты сравнительного анализа первичных последовательностей потенциальных и известных продуктов. Наведено результати молекулярного аналізу пігмент-білкових комплексів і розглянуто особливості ліпідного складу фотосинтетичних мембран Deschampsia antarctica – одного з представників флори Антарктиди. Здійснено біоінформаційний пошук у доступних базах даних продуктів геному Deschampsia antarctica, які структурно та функціонально пов'язані з пластидами. Також здійснено пошук їх гомологів у протеомах Arabidopsis thaliana та Oryza sativa japonica. Наведено результати порівняльного аналізу первинних послідовностей потенційних та відомих продуктів. 2010 Article Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants / N.Yu. Taran, I.P. Ozheredova, V.О. Storozhenko, N.B. Svetlova, N.М. Topchiy // Український антарктичний журнал. — 2010. — № 9. — С. 202-205. — Бібліогр.: 9 назв. — англ. 1727-7485 https://nasplib.isofts.kiev.ua/handle/123456789/128416 577.112.37:115.083:57.045 en Український антарктичний журнал application/pdf Національний антарктичний науковий центр МОН України |
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Біологічні дослідження Біологічні дослідження |
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Біологічні дослідження Біологічні дослідження Taran, N.Yu. Ozheredova, I.P. Storozhenko, V.О. Svetlova, N.B. Topchiy, N.М. Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants Український антарктичний журнал |
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The molecular analysis results of pigment-protein complexes of photosynthetic membranes of Deschampsia antarctica plants, one of the native representatives of the Antarctic region are presented. Bioinformatics searching of genome products of Deschampsia antarctica in available protein databeses related to the structure and function of plastids was carried. The searching of Arabidopsis thaliana and Oryza sativa japonica homolog search submitted in free databases is also performed. The results of comparative analysis of primary sequences of the known and potential products are presented. |
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Article |
| author |
Taran, N.Yu. Ozheredova, I.P. Storozhenko, V.О. Svetlova, N.B. Topchiy, N.М. |
| author_facet |
Taran, N.Yu. Ozheredova, I.P. Storozhenko, V.О. Svetlova, N.B. Topchiy, N.М. |
| author_sort |
Taran, N.Yu. |
| title |
Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants |
| title_short |
Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants |
| title_full |
Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants |
| title_fullStr |
Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants |
| title_full_unstemmed |
Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants |
| title_sort |
pigment-protein complexes of thylakoid membranes of deschampsia antarctica desv. plants |
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Національний антарктичний науковий центр МОН України |
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2010 |
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Біологічні дослідження |
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https://nasplib.isofts.kiev.ua/handle/123456789/128416 |
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Pigment-protein complexes of thylakoid membranes of Deschampsia antarctica Desv. Plants / N.Yu. Taran, I.P. Ozheredova, V.О. Storozhenko, N.B. Svetlova, N.М. Topchiy // Український антарктичний журнал. — 2010. — № 9. — С. 202-205. — Бібліогр.: 9 назв. — англ. |
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PIGMENT-PROTEIN COMPLEXES OF THYLAKOID MEMBRANES OF DESCHAMPSIA
ANTARCTICA DESV. PLANTS
1 1 1 1 2Taran N.Yu. , Ozheredova I.P. , Storozhenko V.О. , Svetlova N.B. , Topchiy N.М.
1 Taras Shevchenko National University of Kyiv, Volodymyrska str,. 64, Kyiv, Ukraine, 01033
tarantul@univ.kiev.ua
2M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Tereshchenkivska
str., 2, Kyiv, Ukraine, 01601
membrana@ukr.net
Abstract. The molecular analysis results of pigment-protein complexes of photosynthetic
membranes of Deschampsia antarctica plants, one of the native representatives of the Antarctic
region are presented. Bioinformatics searching of genome products of Deschampsia antarctica in
available protein databeses related to the structure and function of plastids was carried. The searching
of Arabidopsis thaliana and Oryza sativa japonica homolog search submitted in free databases is also
performed. The results of comparative analysis of primary sequences of the known and potential
products are presented.
Key words: Deschampsia, pigment-protein complexes, proteome.
Реферат. Наведено результати молекулярного аналізу пігмент-білкових комплексів і
розглянуто особливості ліпідного складу фотосинтетичних мембран Deschampsia antarctica –
одного з представників флори Антарктиди. Здійснено біоінформаційний пошук у доступних
базах даних продуктів геному Deschampsia antarctica, які структурно та функціонально
пов'язані з пластидами. Також здійснено пошук їх гомологів у протеомах Arabidopsis thaliana
та Oryza sativa japonica. Наведено результати порівняльного аналізу первинних
послідовностей потенційних та відомих продуктів.
Ключові слова: Deschampsia, пігмент-білкові комплекси, протеом.
Реферат. Приведены результаты молекулярного анализа пигмент-белковых комплексов и
рассмотрены особенности липидного состава фотосинтетических мембран Deschampsia
antarctica – одного из представителей флоры Антарктики. Осуществлен биоинформационный
поиск в доступных базах данных продуктов генома Deschampsia antarctica, которые
структурно и функционально связаны с пластидами. Также осуществлен поиск их гомологов в
протеомах Arabidopsis thaliana и Oryza sativa japonica. Приведены результаты сравнительного
анализа первичных последовательностей потенциальных и известных продуктов.
Ключевые слова: Deschampsia, пигмент-белковые комплексы, протеом.
Introduction
The investigations of protection responses of plants on abiotic stress conditions is actual today.
Antarctic species Deschampsia antarctica Desv. (Poaceae) is unique plant object for studying of
adaptive mechanisms of plants on abiotic stress action. This can be explained both the absence of
breeding and biotechnological interferences in the development of this species and its unique habitat.
УКРАЇНСЬКИЙ АНТАРКТИЧНИЙ
ЖУРНАЛ
УАЖ № 9, 202-205 (2010)
УДК 577.112.37:115.083:57.045
202
203
Phenotypical and genotypic variability are the basis of plant adaptation on abiotic stress. Functioning
of them provides the balance between the requirements of maximal ontogenetic adaptation and
maintenance of phylogenetic flexibility of populations (Жученко, 1994; Кир'яченко, 2005).
Some abiotic factors in Antarctic are excessive illumination and elevated levels of UV-B
radiation. Therefore formation of active paths of adaptation for maintenance of photosynthetic
activity take place both at the level of the ultrastructural organization of organels (Gielwanowska,
2005), that provides effective mechanism of dissipation of excessive energy, and at a biochemical
level of photosynthetic reactions.
Primary processes of photosynthesis take place in thylakoid membranes of chloroplasts during
redox transformations of components of electron transport chain. They involved in adaptive
transformations of photochemical processes. Thylakoid membranes contain five types of protein
complexes, which realize distribution of a charge and transfer of electrons. There are photosystem І
(PS І), photosystem ІІ (PS ІІ), their light-harvesting complexes, cytochrome b /f complexes of proton 6
АТP-synthase (CF -CF ).0 1
The aims of our investigation were isolation and analysis pigment-protein complexes of
thylakoid membranes of chloroplasts and searching in accessible databases of amino acid sequences
of D. antarctica which belong to pigment-protein complexes of thylakoid membranes and also
carrying out of their comparative analysis with Arabidopsis thaliana (L.) and Oryza sativa L. ssp.
japonica cv. Nipponbare sequences for finding-out of particularities of formation of adaptive
reactions of D. antarctica plants at a level of primary processes of photosynthesis.
Materials and methods
The analysis of pigment-protein complexes of thylakoid membranes carried out by method of
nondenaturing polyacrylamide gel electrophoresis in polyacrylamide gel with using of sodium lauryl
sulfate (SLS) for decomposition of membranes desintegration (Anderson, 1980). The relative
pigment-protein content was estimated by chlorophyll concentration that was determined in 80 %
acetone (Arnon, 1949). Electrophoregrams scaned ('Mustek', China) and then analyzed by TotalLab
V1.10.
Searching of D. antarctica sequences was spent in open databases Swiss-prot / TREMBL
(http://www.expasy.org). Searching of potential homologues was carried out by comparison of
D. antarctica sequences with proteomes of A. thaliana and O. sativa, as vegetative organisms which
are most full presented in databases. Searching of homologous sequences and determination of a
homologous level carried out with BLASTp tool (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi). The
selection of homologous sequences for further analysis was spent by standard method according to
-3which proteins is homologous under identity ≥ 25 % with E-value <10 . Global equalization of
protein sequences carried out with on-line resource Clustal with using of BLOSUM matrix. The
analysis of domain architecture of amino acid sequences spent with on-line resource ART
(http: //smart.embl.de).
Results and discussion
We identified seven strips according to non-denaturing green SDS-electrophoresis. Each of them
associated with pigment-protein complexes according to Anderson nomenclature (Anderson, 1980)
(fig. 1).
In result of comparison of pigment-protein complexes of thylakoid membranes of D. antarctica
plants with Pisum sativum L. plants (Топчій, 2006) the essential qualitative differences between them
were not exposed.
Taran N.Yu.: PIGMENT-PROTEIN COMPLEXES OF THYLAKOID MEMBRANES OF...
204
Quantitative differences in general content of LHCII (oligomerous (LHCP1) and monomerous
(LHCP3) forms), chlorophyll content in СРа, (protein complexes of complexes of near antenna) are
revealed. In our opinion, quantitative differences associated with features of growth conditions of D.
antarctica plants. Thus, the influences of excessive light and increased level of UV-B radiation.
The relative content of pigment-protein complexes of thylakoid membranes of D. antarctica
plants is presented lower. СР1а and СР1 is 8,97 ± 0,38 (%) and 12,34 ± 0,26 (%), oligomerous form
(LHCP1) of LHCII - 27,02 ± 0,53 (%), whereas monomeric form LHCP3) - 20,16 ± 0,61 (%). The
relation between forms (LHCP 1/LHCP3) is equaled 1,34 ± 0,02, general content of LHC ІІ (LHCP1 +
LHCP3) is 47,17 ± 1,11 (%).
Scanning of databases has shown, that 37 amino acid sequences which belong to D. antarctica
proteome currently there is in free access. From them 20 sequences are potential proteins or fragments
of proteins with specified function and 17 are potential proteins or fragments of proteins which
function is not determined. Thus, annotation of D. antarctica genome and proteome is on the initial
stage of determination of function of all known proteins, and functions practically all known potential
proteins are not determined finally.
In our work special attention pays to plastid proteins. We selected from databases 15 proteins,
which had chloroplast origin and related to families RsbG and PsbC (Nixon, 1989, Luciski, 2006).
The comparison of Psb sequences of D. antarctica received from databases (ABS30954;
ABS30955; ABS30956; ABS30957; ABS30958) demonstrated, that they are identical, and they are
the sequencing of one potential product ('Clustal' program). The analysis of domain architecture of
this fragment was conducted by online SMART recourse. It confirmed the accessory of this fragment
to PsbC . The determination of fragment of protein domain architecture of Psb was impossible for D.
antarctica plants. It associated with small sizes of amino-acid residues.
Searching of homological sequences in A.thaliana and O.sativa proteomes was lead (BLASTp
instrument) for specification of function of D. antarctica hypothetical product. As result, have been
allocated amino acid sequences NP051055 A.thaliana (identity of 97 %, E-value 0,00) and NP039367
O.sativa (identity of 98 %, E-value 0,00). In articles of databases they are presented proteins of PsbC
Fig. 1. Electrophoregram of
pigment-protein complexes of
thylakoid membranes.
CP1а - complex of RC of PS I,
which partially have own light-
harvesting complex (LHC І);
СР1 - complex of RC of PS I
1without LHC І; LHCP -
2oligomeric form of LHCII; LHCP
– LHC І;
СРа - the nearest to RC of PS ІІ
light-harvesting pigment-protein
complex;
3LHCP – monomeric form of LHC
ІІ; FP - free chlorophyll
Taran N.Yu.: PIGMENT-PROTEIN COMPLEXES OF THYLAKOID MEMBRANES OF...
205
family are a part and participate in binding of chlorophyll and β-carotene and transfer of excitation
energy to reaction center (Barber, 2002).
Multiple global alignment of found sequences of A. thaliana and O. sativa with fragment has
revealed the significant level of conservatism of this proteins (fig. 2).
Fig. 2. Comparison of amino acid sequences of PS II protein fractions of D. antarctica, A. thaliana and
O. sativa.
The structured area of these proteins includes from 31 to 473 amino-acid residues. The investigated
fragment of D. antarctica sequence practically completely coincides with the sequence of proteins from 45 to 473
amino acid residue. But positions 106, 170, 177, 185, 245, 264, 427, 437, 463 had strong replacements, on 207,
277, 470 positions was weak replacement, and on 242, 282, 468 coincidence was absent. Probably strong
replacements lead to the certain modifications in structure of this protein which provides the effective mechanism
of excessive energy dissipation for maintenance photosynthesis in normal conditions.
Conclusions
Quantitative differences in general content of LHC II (oligomeric and monomeric forms), chlorophyll
content in СРа zone (pigment-protein complexes of nearest antenna) are revealed. Results of global alignment
and domain architecture have shown, that analyzed fragment of amino acid sequence of PS II protein fraction of
D. antarctica has high affinity with sequences of A. thaliana and O. sativa which belong to РsbС family of
proteins, and it is a fragment of a product of a gene of psbС family. Also it is possible to note, that in the given
fragment is absent C-final sequence, which have size as one or several exons.
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