Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposu...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2009 |
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| Sprache: | Englisch |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862714254583398400 |
|---|---|
| author | Rusetskaya, N.V. Lukyanova, N.Yu. Chekhun, V.F. |
| author_facet | Rusetskaya, N.V. Lukyanova, N.Yu. Chekhun, V.F. |
| citation_txt | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dох) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of ΜCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dох cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dох to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines. Conclusion: The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dох cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases.
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| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-134401 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T17:49:27Z |
| publishDate | 2009 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Rusetskaya, N.V. Lukyanova, N.Yu. Chekhun, V.F. 2018-06-13T11:45:02Z 2018-06-13T11:45:02Z 2009 Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/134401 Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dох) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of ΜCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dох cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dох to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines. Conclusion: The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dох cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin Article published earlier |
| spellingShingle | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin Rusetskaya, N.V. Lukyanova, N.Yu. Chekhun, V.F. Original contributions |
| title | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin |
| title_full | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin |
| title_fullStr | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin |
| title_full_unstemmed | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin |
| title_short | Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin |
| title_sort | molecular profile and cell cycle in mcf-7 and mcf‑7/dox cells exposed to conventional and liposomal forms of doxorubicin |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/134401 |
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