Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin

Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin

Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposu...

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Published in:Experimental Oncology
Date:2009
Main Authors: Rusetskaya, N.V., Lukyanova, N.Yu., Chekhun, V.F.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/134401
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-134401
record_format dspace
spelling Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
2018-06-13T11:45:02Z
2018-06-13T11:45:02Z
2009
Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/134401
Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dох) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of ΜCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dох cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dох to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines. Conclusion: The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dох cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
spellingShingle Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
Original contributions
title_short Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_full Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_fullStr Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_full_unstemmed Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin
title_sort molecular profile and cell cycle in mcf-7 and mcf‑7/dox cells exposed to conventional and liposomal forms of doxorubicin
author Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
author_facet Rusetskaya, N.V.
Lukyanova, N.Yu.
Chekhun, V.F.
topic Original contributions
topic_facet Original contributions
publishDate 2009
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin. Methods: МТТ-test, immunocytochemistry, flow cytometry. Results: In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dох) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of ΜCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dох cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dох to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines. Conclusion: The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dох cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/134401
citation_txt Molecular profile and cell cycle in MCF-7 and MCF‑7/Dox cells exposed to conventional and liposomal forms of doxorubicin / N.V. Rusetskaya, N.Yu. Lukyanova, V.F. Chekhun // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 140-143. — Бібліогр.: 11 назв. — англ.
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AT chekhunvf molecularprofileandcellcycleinmcf7andmcf7doxcellsexposedtoconventionalandliposomalformsofdoxorubicin
first_indexed 2025-12-07T17:49:27Z
last_indexed 2025-12-07T17:49:27Z
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