Molecular and biological characterization of low-pathogenic H9N2 influenza viruses isolated in Israel

• Datum: 2015
• Hauptverfasser: Tendler, Ye., Golender, N., Shkoda, I., Drabkin, M., Lapin, K., Panshin, A.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Фізико-хімічний інститут ім. О.В. Богатського НАН України 2015
• Schriftenreihe: Актуальні проблеми транспортної медицини
• Schlagworte: Экспериментальные исследования
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/134724
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Molecular and biological characterization of low-pathogenic H9N2 influenza viruses isolated in Israel / Ye. Tendler, N. Golender, I. Shkoda, M. Drabkin, K. Lapin, A. Panshin // Актуальні проблеми транспортної медицини. — 2015. — № 1 (39). — С. 112-136. — Бібліогр.: 29 назв. — англ.

All Israeli H9N2-strain viruses used in the present study were subdivided among three groups: groups A and B viruses contained – in all eight segments – 90658/00-like and 1304/03-like sequences, respectively; Group C comprised viruses isolated in 2006-2010, which contained four 1304/03-like segments and four 1525/06-like segments. Molecular analysis revealed that most of the isolates had an RSSR motif at the cleavage site of haemagglutinin. Most of the viruses had an haemagglutinin with L216 typical of humans, and internal proteins associated with the avian host specificity. The studied viruses infected the majority of the directly inoculated birds. Exposure to the virus with full8length PB1-F2 protein for 24 h caused destruction of mitochondria and cell death in cultures of human macrophages, and this effect was not associated with activation of protein p53. A virus with truncated PB1-F2 protein exhibited no destructive effect on mitochondria, but induced enhanced production of pro-apoptotic P53 protein