Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR
Aim: To determine and compare cytokeratin 20 (CK20) mRNA expression in urine of patients with transitional cell carcinomas of bladder (TCCB), urological benign diseases, and healthy volunteers. Methods: Taqman probe was designed according to the sequence of CK20 cloned gene. The quantitative PCR r...
Saved in:
| Published in: | Experimental Oncology |
|---|---|
| Date: | 2009 |
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
|
| Subjects: | |
| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/134932 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR / B. Guo1, C. Luo, C. Xun, J. Xie, X. Wu, J. Pu // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 43-47. — Бібліогр.: 21 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
nasplib_isofts_kiev_ua-123456789-134932 |
|---|---|
| record_format |
dspace |
| spelling |
Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. 2018-06-14T11:42:00Z 2018-06-14T11:42:00Z 2009 Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR / B. Guo1, C. Luo, C. Xun, J. Xie, X. Wu, J. Pu // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 43-47. — Бібліогр.: 21 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/134932 Aim: To determine and compare cytokeratin 20 (CK20) mRNA expression in urine of patients with transitional cell carcinomas of bladder (TCCB), urological benign diseases, and healthy volunteers. Methods: Taqman probe was designed according to the sequence of CK20 cloned gene. The quantitative PCR reaction system was optimized and evaluated. The CK20 mRNA level was screened by real-time polymerase chain reaction (RT-PCR) in 95 urine samples and analyzed according to the following parameters: urinary cytology, nuclear matrix protein 22 (NMP22) expression, tumor stage and grade. Results: For 60 TCCB patients urinary cytology was positive in 28 (46.7%), control group had no false-positive results (specificity 100%). CK20 expression was positive in RT-PCR of 51 cases (85%) of TCCB, but control group was positive in 2 cases (specificity 94.3%) with a cutoff value of crossover point (CT) = 30. Two methods have significant variation in sensitivity (p < 0.001), NMP22 expression was positive in 47 cases (78.3%), but control group was positive in 9 cases (specificity 85%). In the simultaneous evaluation of CK20 and NMP22 mRNA expression, there were 54 positive cases (90%). CK20 mRNA values in TCCB group (mean 27712.57 copies/µl) were significantly higher than in non-cancer disease urological group (mean 74.45 copies/µl) and control group (mean 8.47 copies/µl) (p < 0.001, p < 0.001, respectively). CK20 mRNA values increased gradually with higher tumor grade and stage: G1 differs significantly from G2 (p = 0.016); Tis/Ta differs significantly from T1–2 (p = 0.022). Conclusion: Our results indicate that CK20 mRNA expression could be regarded as a potential marker for TCCB. We demonstrated correlation between CK20 expression and the clinicopathologic features of TCCB (tumor stage and grade); simultaneous use of CK20 and NMP22 markers will elevate the sensitivity of the method. CK20 RT-PCR is a sensitive, quantitative, rapid and specific method to detect free cancer cells in the urine, and could be recommended for be wide application in the diagnostics of TCCB and evaluation of therapeutic effect. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| spellingShingle |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. Original contributions |
| title_short |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_full |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_fullStr |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_full_unstemmed |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_sort |
quantitative detection of cytokeratin 20 mrna in urine samples as diagnostic tools for bladder cancer by real-time pcr |
| author |
Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. |
| author_facet |
Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. |
| topic |
Original contributions |
| topic_facet |
Original contributions |
| publishDate |
2009 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
Aim: To determine and compare cytokeratin 20 (CK20) mRNA expression in urine of patients with transitional cell carcinomas
of bladder (TCCB), urological benign diseases, and healthy volunteers. Methods: Taqman probe was designed according to the
sequence of CK20 cloned gene. The quantitative PCR reaction system was optimized and evaluated. The CK20 mRNA level was
screened by real-time polymerase chain reaction (RT-PCR) in 95 urine samples and analyzed according to the following parameters:
urinary cytology, nuclear matrix protein 22 (NMP22) expression, tumor stage and grade. Results: For 60 TCCB patients urinary
cytology was positive in 28 (46.7%), control group had no false-positive results (specificity 100%). CK20 expression was positive in
RT-PCR of 51 cases (85%) of TCCB, but control group was positive in 2 cases (specificity 94.3%) with a cutoff value of crossover
point (CT) = 30. Two methods have significant variation in sensitivity (p < 0.001), NMP22 expression was positive in 47 cases
(78.3%), but control group was positive in 9 cases (specificity 85%). In the simultaneous evaluation of CK20 and NMP22 mRNA
expression, there were 54 positive cases (90%). CK20 mRNA values in TCCB group (mean 27712.57 copies/µl) were significantly
higher than in non-cancer disease urological group (mean 74.45 copies/µl) and control group (mean 8.47 copies/µl) (p < 0.001,
p < 0.001, respectively). CK20 mRNA values increased gradually with higher tumor grade and stage: G1 differs significantly from
G2 (p = 0.016); Tis/Ta
differs significantly from T1–2 (p = 0.022). Conclusion: Our results indicate that CK20 mRNA expression
could be regarded as a potential marker for TCCB. We demonstrated correlation between CK20 expression and the clinicopathologic
features of TCCB (tumor stage and grade); simultaneous use of CK20 and NMP22 markers will elevate the sensitivity of the
method. CK20 RT-PCR is a sensitive, quantitative, rapid and specific method to detect free cancer cells in the urine, and could be
recommended for be wide application in the diagnostics of TCCB and evaluation of therapeutic effect.
|
| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/134932 |
| citation_txt |
Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR / B. Guo1, C. Luo, C. Xun, J. Xie, X. Wu, J. Pu // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 43-47. — Бібліогр.: 21 назв. — англ. |
| work_keys_str_mv |
AT guob quantitativedetectionofcytokeratin20mrnainurinesamplesasdiagnostictoolsforbladdercancerbyrealtimepcr AT luoc quantitativedetectionofcytokeratin20mrnainurinesamplesasdiagnostictoolsforbladdercancerbyrealtimepcr AT xunc quantitativedetectionofcytokeratin20mrnainurinesamplesasdiagnostictoolsforbladdercancerbyrealtimepcr AT xiej quantitativedetectionofcytokeratin20mrnainurinesamplesasdiagnostictoolsforbladdercancerbyrealtimepcr AT wux quantitativedetectionofcytokeratin20mrnainurinesamplesasdiagnostictoolsforbladdercancerbyrealtimepcr AT puj quantitativedetectionofcytokeratin20mrnainurinesamplesasdiagnostictoolsforbladdercancerbyrealtimepcr |
| first_indexed |
2025-11-27T10:29:15Z |
| last_indexed |
2025-11-27T10:29:15Z |
| _version_ |
1850852092918366208 |