Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR
Aim: To determine and compare cytokeratin 20 (CK20) mRNA expression in urine of patients with transitional cell carcinomas
 of bladder (TCCB), urological benign diseases, and healthy volunteers. Methods: Taqman probe was designed according to the
 sequence of CK20 cloned gene. The qu...
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| Published in: | Experimental Oncology |
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| Date: | 2009 |
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/134932 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR / B. Guo1, C. Luo, C. Xun, J. Xie, X. Wu, J. Pu // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 43-47. — Бібліогр.: 21 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862593748605599744 |
|---|---|
| author | Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. |
| author_facet | Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. |
| citation_txt | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR / B. Guo1, C. Luo, C. Xun, J. Xie, X. Wu, J. Pu // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 43-47. — Бібліогр.: 21 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To determine and compare cytokeratin 20 (CK20) mRNA expression in urine of patients with transitional cell carcinomas
of bladder (TCCB), urological benign diseases, and healthy volunteers. Methods: Taqman probe was designed according to the
sequence of CK20 cloned gene. The quantitative PCR reaction system was optimized and evaluated. The CK20 mRNA level was
screened by real-time polymerase chain reaction (RT-PCR) in 95 urine samples and analyzed according to the following parameters:
urinary cytology, nuclear matrix protein 22 (NMP22) expression, tumor stage and grade. Results: For 60 TCCB patients urinary
cytology was positive in 28 (46.7%), control group had no false-positive results (specificity 100%). CK20 expression was positive in
RT-PCR of 51 cases (85%) of TCCB, but control group was positive in 2 cases (specificity 94.3%) with a cutoff value of crossover
point (CT) = 30. Two methods have significant variation in sensitivity (p < 0.001), NMP22 expression was positive in 47 cases
(78.3%), but control group was positive in 9 cases (specificity 85%). In the simultaneous evaluation of CK20 and NMP22 mRNA
expression, there were 54 positive cases (90%). CK20 mRNA values in TCCB group (mean 27712.57 copies/µl) were significantly
higher than in non-cancer disease urological group (mean 74.45 copies/µl) and control group (mean 8.47 copies/µl) (p < 0.001,
p < 0.001, respectively). CK20 mRNA values increased gradually with higher tumor grade and stage: G1 differs significantly from
G2 (p = 0.016); Tis/Ta
differs significantly from T1–2 (p = 0.022). Conclusion: Our results indicate that CK20 mRNA expression
could be regarded as a potential marker for TCCB. We demonstrated correlation between CK20 expression and the clinicopathologic
features of TCCB (tumor stage and grade); simultaneous use of CK20 and NMP22 markers will elevate the sensitivity of the
method. CK20 RT-PCR is a sensitive, quantitative, rapid and specific method to detect free cancer cells in the urine, and could be
recommended for be wide application in the diagnostics of TCCB and evaluation of therapeutic effect.
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| first_indexed | 2025-11-27T10:29:15Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-134932 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-27T10:29:15Z |
| publishDate | 2009 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. 2018-06-14T11:42:00Z 2018-06-14T11:42:00Z 2009 Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR / B. Guo1, C. Luo, C. Xun, J. Xie, X. Wu, J. Pu // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 43-47. — Бібліогр.: 21 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/134932 Aim: To determine and compare cytokeratin 20 (CK20) mRNA expression in urine of patients with transitional cell carcinomas
 of bladder (TCCB), urological benign diseases, and healthy volunteers. Methods: Taqman probe was designed according to the
 sequence of CK20 cloned gene. The quantitative PCR reaction system was optimized and evaluated. The CK20 mRNA level was
 screened by real-time polymerase chain reaction (RT-PCR) in 95 urine samples and analyzed according to the following parameters:
 urinary cytology, nuclear matrix protein 22 (NMP22) expression, tumor stage and grade. Results: For 60 TCCB patients urinary
 cytology was positive in 28 (46.7%), control group had no false-positive results (specificity 100%). CK20 expression was positive in
 RT-PCR of 51 cases (85%) of TCCB, but control group was positive in 2 cases (specificity 94.3%) with a cutoff value of crossover
 point (CT) = 30. Two methods have significant variation in sensitivity (p < 0.001), NMP22 expression was positive in 47 cases
 (78.3%), but control group was positive in 9 cases (specificity 85%). In the simultaneous evaluation of CK20 and NMP22 mRNA
 expression, there were 54 positive cases (90%). CK20 mRNA values in TCCB group (mean 27712.57 copies/µl) were significantly
 higher than in non-cancer disease urological group (mean 74.45 copies/µl) and control group (mean 8.47 copies/µl) (p < 0.001,
 p < 0.001, respectively). CK20 mRNA values increased gradually with higher tumor grade and stage: G1 differs significantly from
 G2 (p = 0.016); Tis/Ta
 differs significantly from T1–2 (p = 0.022). Conclusion: Our results indicate that CK20 mRNA expression
 could be regarded as a potential marker for TCCB. We demonstrated correlation between CK20 expression and the clinicopathologic
 features of TCCB (tumor stage and grade); simultaneous use of CK20 and NMP22 markers will elevate the sensitivity of the
 method. CK20 RT-PCR is a sensitive, quantitative, rapid and specific method to detect free cancer cells in the urine, and could be
 recommended for be wide application in the diagnostics of TCCB and evaluation of therapeutic effect. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR Article published earlier |
| spellingShingle | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR Guo, B. Luo, C. Xun, C. Xie, J. Wu, X. Pu, J. Original contributions |
| title | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_full | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_fullStr | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_full_unstemmed | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_short | Quantitative detection of cytokeratin 20 mRNA in urine samples as diagnostic tools for bladder cancer by real-time PCR |
| title_sort | quantitative detection of cytokeratin 20 mrna in urine samples as diagnostic tools for bladder cancer by real-time pcr |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/134932 |
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