synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age
Aim: To develop a rationally designed new organotin compound namely dimethyl tin 4-cyclohexyl thiosemicarbazone (D4-t) and
 evaluate its putative antitumor activity. Methods: Starting from 4-cyclohexyl thiosemicarbazone, a three step synthetic procedure was
 followed to obtain the ti...
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| Опубліковано в: : | Experimental Oncology |
|---|---|
| Дата: | 2009 |
| Автори: | , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/135103 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age / A. Sen, T.K. Chaudhuri // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 22-26. — Бібліогр.: 24 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862652751789424640 |
|---|---|
| author | Sen, A. Chaudhuri, T.K. |
| author_facet | Sen, A. Chaudhuri, T.K. |
| citation_txt | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age / A. Sen, T.K. Chaudhuri // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 22-26. — Бібліогр.: 24 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To develop a rationally designed new organotin compound namely dimethyl tin 4-cyclohexyl thiosemicarbazone (D4-t) and
evaluate its putative antitumor activity. Methods: Starting from 4-cyclohexyl thiosemicarbazone, a three step synthetic procedure was
followed to obtain the title compound. In vivo lymphocyte activation property of the compound at three different doses was assayed
by measuring the blastogenesis. Concanavalin A (ConA) was used as standard mitogen for murine T cells stimulation in vivo. Also,
the synthesis of DNA by the activated lymphocytes was measured after injecting the D4-t. The lymphocyte activation property and
antitumor efficacy of D4-twere assessed inSarcoma-180 (S-180) bearing mice. The organization of lymphoid cells was studied in the
histological preparations ofspleen and mesenteric lymph node. Tumor neutralization assay (Winn assay) was conducted to examine
whether immune responses were associated with the manifestation of antitumor efficacies of this compound in S-180 in vivo. The
DNA synthesis inhibitory effect of the compound in S-180 cells was studied in vitro, and was found significant (P < 0.001). Results:
Different doses of the new compound caused differential response of blastogenesis and DNA synthesis. In comparison to ConA, the
title compound showed a good number of blast cells at its optimum dose of 5 mg/kg. It caused maximum synthesis of DNA by the
lymphoid cells. In histological preparations, the gradual transformation of lymphocytes into blasts was observed without any visible
toxicity. Winn assay revealed that 5 mg/kg of D4-t was able to reduce tumor mass without severe toxicity. This organotin compound
also inhibits the synthesis of DNA in S-180 tumor cells in comparison to Platin10 and ConA. Conclusion: The title compound has
the lymphocyte activation property and stimulates immune response of the lymphoid cells, which in turn express the antitumor
activity without any significant toxicity. Results indicate promising therapeutic potential of D4-t.
|
| first_indexed | 2025-12-01T23:07:02Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-135103 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-01T23:07:02Z |
| publishDate | 2009 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Sen, A. Chaudhuri, T.K. 2018-06-14T15:24:15Z 2018-06-14T15:24:15Z 2009 synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age / A. Sen, T.K. Chaudhuri // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 22-26. — Бібліогр.: 24 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/135103 Aim: To develop a rationally designed new organotin compound namely dimethyl tin 4-cyclohexyl thiosemicarbazone (D4-t) and
 evaluate its putative antitumor activity. Methods: Starting from 4-cyclohexyl thiosemicarbazone, a three step synthetic procedure was
 followed to obtain the title compound. In vivo lymphocyte activation property of the compound at three different doses was assayed
 by measuring the blastogenesis. Concanavalin A (ConA) was used as standard mitogen for murine T cells stimulation in vivo. Also,
 the synthesis of DNA by the activated lymphocytes was measured after injecting the D4-t. The lymphocyte activation property and
 antitumor efficacy of D4-twere assessed inSarcoma-180 (S-180) bearing mice. The organization of lymphoid cells was studied in the
 histological preparations ofspleen and mesenteric lymph node. Tumor neutralization assay (Winn assay) was conducted to examine
 whether immune responses were associated with the manifestation of antitumor efficacies of this compound in S-180 in vivo. The
 DNA synthesis inhibitory effect of the compound in S-180 cells was studied in vitro, and was found significant (P < 0.001). Results:
 Different doses of the new compound caused differential response of blastogenesis and DNA synthesis. In comparison to ConA, the
 title compound showed a good number of blast cells at its optimum dose of 5 mg/kg. It caused maximum synthesis of DNA by the
 lymphoid cells. In histological preparations, the gradual transformation of lymphocytes into blasts was observed without any visible
 toxicity. Winn assay revealed that 5 mg/kg of D4-t was able to reduce tumor mass without severe toxicity. This organotin compound
 also inhibits the synthesis of DNA in S-180 tumor cells in comparison to Platin10 and ConA. Conclusion: The title compound has
 the lymphocyte activation property and stimulates immune response of the lymphoid cells, which in turn express the antitumor
 activity without any significant toxicity. Results indicate promising therapeutic potential of D4-t. We sincerely thank the Department of Science &
 Technology (DST), Women Scientists Scheme (WOS-A),
 New Delhi, India, (No.SR/WOS-A/CS-07/2004) for
 financial assistance to Dr. A. Sen. Also we thank
 to Dr. U. Sanyal, A. Mukherjee and S. Dutta of Dept.
 ofACDD & Chemotherapy, Chittaranjan National Cancer
 Institute, Kolkata for their immense help and support
 in performing all the radioactive assays for the project. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age Article published earlier |
| spellingShingle | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age Sen, A. Chaudhuri, T.K. Original contributions |
| title | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age |
| title_full | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age |
| title_fullStr | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age |
| title_full_unstemmed | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age |
| title_short | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age |
| title_sort | synthesis and evaluation of dimethyl tin 4-cyclohexyl thiosemicarbazone as a novel antitumor age |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/135103 |
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