Effects of low and high concentrations of antitumour drug taxol in anaplastic thyroid cancer cells

• Опубліковано в: : Experimental Oncology
• Дата: 2009
• Автори: Pushkarev, V.M., Starenki, D.V., Saenko, V.A., Yamashita, S., Kovzun, O.I., Popadiuk, I.D., Pushkarev, V.V., Tronko, M.D.
• Формат: Стаття
• Мова: Англійська
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
• Теми: Original contributions
• Онлайн доступ: https://nasplib.isofts.kiev.ua/handle/123456789/135106
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Effects of low and high concentrations of antitumour drug taxol in anaplastic thyroid cancer cells / V.M. Pushkarev, D.V. Starenki, V.A. Saenko, S. Yamashita, O.I. Kovzun, I.D. Popadiuk, V.V. Pushkarev, M.D. Tronko // Experimental Oncology. — 2009. — Т. 31, № 1. — С. 16-21. — Бібліогр.: 17 назв. — англ.

Aim: To study the changes of cell cycle, mitochondrial membrane potential and caspase activation in response to an antitumour drug
 Taxol in ARO and KTC-2 cell lines of anaplastic thyroid carcinoma. Methods: Experiments were done on thyroid anaplastic cancer
 cell lines ARO and KTC-2 using Western blotting, flow cytometry, light and fluorescent microscopy. Results: Taxol significantly
 activated caspases in ARO cells starting from drug concentration of 5 nM. Maximum activation was observed at 25 nM and further
 increase of Taxol concentration to 100 nM resulted in a reduction of caspase activation. Concomitant to caspase activation,
 a loss of mitochondrial membrane potential was observed. At Taxol concentration of 100 nM, most cells lost their mitochondrial
 membrane potential. Low Taxol concentrations (10 nM) caused changes in the cell cycle that are typical for apoptosis without cell
 cycle arrest. Higher drug doses starting from 50 nM arrested cell cycle in G2/M phase. In KTC-2 cell line Taxol concentration as
 low as 1 nM induced apoptosis. 6–15 nM of the drug caused massive (75–83%) cell death. Upon Taxol action, the increase in the
 number of cells displaying manifestations of accelerated senescence was insignificant. Conclusion: Taxol induces bona fide apoptosis
 in thyroid cancer cell cultures at low (1–25 nM) concentrations. Higher drug doses cause the loss of mitochondrial membrane
 potential and possibly lead to other types of cell death. No accelerated senescence at different Taxol concentrations was observed.
 The significance of subG1 and G2/M cell populations at low and high doses of Taxol is discussed.