Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1

• Published in: Experimental Oncology
• Date: 2009
• Main Authors: Magorivska, I., Bilyy, R., Shalay, O., Loginsky, V., Kit, Y., Stoika, R.
• Format: Article
• Language: English
• Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
• Subjects: Original contributions
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/135699
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 / I. Magorivska, R. Bilyy, O. Shalay, V. Loginsky, Y. Kit1, R. Stoika // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 97-101. — Бібліогр.: 30 назв. — англ.

Background: Recently we have shown that the imunoglobulins G from blood serum of some multiple sclerosis patients are capable
 of cleaving histone H1. Aim: To check whether histone H1-hydrolyzing abzymes could be detected not only in blood plasma of autoimmune
 patients, but also during cancer development, particularly during the onset of multiple myeloma. Methods: Immunoglobulines
 were isolated from blood serum of multiple myeloma patients (n = 11) by precipitation with 50% ammonium sulfate and tested for
 proteolytic activity toward linker and core calf thymus histones. Antibody preparations able to cleaved histone H1 were subjected
 to affinity chromatography on histone H1-Sepharose with following analysis of chromatographic fractions’ protease activity. To
 prove that antibody molecules are responsible for hydrolysis of histone H1, gel filtration at acidic pH with subsequent examination
 of protease activity of chromatographic fractions (pH-shock analysis) was used. Results: It was found that 3 of 11 antibody
 preparations are capable of hydrolyzing calf thymus histone H1 but not core histones. It was shown that histone H1-hydrolysing
 activity of 2 proteolytically active antibody preparations is associated with IgGs that possess affinity towards histone H1. pH-shock
 analysis proved that protease activity towards histone H1 is intrinsic property of IgG molecules. Conclusions: We demonstrated the
 existence of previously unknown histone H1 hydrolyzing IgG abzymes in the serum of multiple myeloma patients. Possible biological
 role of hisH1-hydrolyzing antibodies in patients with multiple myeloma was discussed.