Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1
Background: Recently we have shown that the imunoglobulins G from blood serum of some multiple sclerosis patients are capable of cleaving histone H1. Aim: To check whether histone H1-hydrolyzing abzymes could be detected not only in blood plasma of autoimmune patients, but also during cancer devel...
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| Published in: | Experimental Oncology |
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| Date: | 2009 |
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| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/135699 |
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| Cite this: | Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 / I. Magorivska, R. Bilyy, O. Shalay, V. Loginsky, Y. Kit1, R. Stoika // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 97-101. — Бібліогр.: 30 назв. — англ. |
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Magorivska, I. Bilyy, R. Shalay, O. Loginsky, V. Kit, Y. Stoika, R. 2018-06-15T14:02:10Z 2018-06-15T14:02:10Z 2009 Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 / I. Magorivska, R. Bilyy, O. Shalay, V. Loginsky, Y. Kit1, R. Stoika // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 97-101. — Бібліогр.: 30 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/135699 Background: Recently we have shown that the imunoglobulins G from blood serum of some multiple sclerosis patients are capable of cleaving histone H1. Aim: To check whether histone H1-hydrolyzing abzymes could be detected not only in blood plasma of autoimmune patients, but also during cancer development, particularly during the onset of multiple myeloma. Methods: Immunoglobulines were isolated from blood serum of multiple myeloma patients (n = 11) by precipitation with 50% ammonium sulfate and tested for proteolytic activity toward linker and core calf thymus histones. Antibody preparations able to cleaved histone H1 were subjected to affinity chromatography on histone H1-Sepharose with following analysis of chromatographic fractions’ protease activity. To prove that antibody molecules are responsible for hydrolysis of histone H1, gel filtration at acidic pH with subsequent examination of protease activity of chromatographic fractions (pH-shock analysis) was used. Results: It was found that 3 of 11 antibody preparations are capable of hydrolyzing calf thymus histone H1 but not core histones. It was shown that histone H1-hydrolysing activity of 2 proteolytically active antibody preparations is associated with IgGs that possess affinity towards histone H1. pH-shock analysis proved that protease activity towards histone H1 is intrinsic property of IgG molecules. Conclusions: We demonstrated the existence of previously unknown histone H1 hydrolyzing IgG abzymes in the serum of multiple myeloma patients. Possible biological role of hisH1-hydrolyzing antibodies in patients with multiple myeloma was discussed. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 Article published earlier |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 |
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Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 Magorivska, I. Bilyy, R. Shalay, O. Loginsky, V. Kit, Y. Stoika, R. Original contributions |
| title_short |
Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 |
| title_full |
Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 |
| title_fullStr |
Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 |
| title_full_unstemmed |
Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 |
| title_sort |
blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone h1 |
| author |
Magorivska, I. Bilyy, R. Shalay, O. Loginsky, V. Kit, Y. Stoika, R. |
| author_facet |
Magorivska, I. Bilyy, R. Shalay, O. Loginsky, V. Kit, Y. Stoika, R. |
| topic |
Original contributions |
| topic_facet |
Original contributions |
| publishDate |
2009 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
Background: Recently we have shown that the imunoglobulins G from blood serum of some multiple sclerosis patients are capable
of cleaving histone H1. Aim: To check whether histone H1-hydrolyzing abzymes could be detected not only in blood plasma of autoimmune
patients, but also during cancer development, particularly during the onset of multiple myeloma. Methods: Immunoglobulines
were isolated from blood serum of multiple myeloma patients (n = 11) by precipitation with 50% ammonium sulfate and tested for
proteolytic activity toward linker and core calf thymus histones. Antibody preparations able to cleaved histone H1 were subjected
to affinity chromatography on histone H1-Sepharose with following analysis of chromatographic fractions’ protease activity. To
prove that antibody molecules are responsible for hydrolysis of histone H1, gel filtration at acidic pH with subsequent examination
of protease activity of chromatographic fractions (pH-shock analysis) was used. Results: It was found that 3 of 11 antibody
preparations are capable of hydrolyzing calf thymus histone H1 but not core histones. It was shown that histone H1-hydrolysing
activity of 2 proteolytically active antibody preparations is associated with IgGs that possess affinity towards histone H1. pH-shock
analysis proved that protease activity towards histone H1 is intrinsic property of IgG molecules. Conclusions: We demonstrated the
existence of previously unknown histone H1 hydrolyzing IgG abzymes in the serum of multiple myeloma patients. Possible biological
role of hisH1-hydrolyzing antibodies in patients with multiple myeloma was discussed.
|
| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/135699 |
| citation_txt |
Blood serum immunoglobulins of patients with multiple myeloma are capable of hydrolysing histone H1 / I. Magorivska, R. Bilyy, O. Shalay, V. Loginsky, Y. Kit1, R. Stoika // Experimental Oncology. — 2009. — Т. 31, № 2. — С. 97-101. — Бібліогр.: 30 назв. — англ. |
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| first_indexed |
2025-12-07T17:25:02Z |
| last_indexed |
2025-12-07T17:25:02Z |
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