Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells
The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammoni...
Gespeichert in:
| Veröffentlicht in: | Experimental Oncology |
|---|---|
| Datum: | 2017 |
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
|
| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/137602 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ. |
Institution
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
nasplib_isofts_kiev_ua-123456789-137602 |
|---|---|
| record_format |
dspace |
| spelling |
Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. 2018-06-17T13:54:51Z 2018-06-17T13:54:51Z 2017 Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/137602 The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells Article published earlier |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| title |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| spellingShingle |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. Original contributions |
| title_short |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_full |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_fullStr |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_full_unstemmed |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_sort |
anti-histone h1 iggs possess proliferative activity towards human t-leukaemia cem cells |
| author |
Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. |
| author_facet |
Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. |
| topic |
Original contributions |
| topic_facet |
Original contributions |
| publishDate |
2017 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens.
|
| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/137602 |
| citation_txt |
Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ. |
| work_keys_str_mv |
AT kityu antihistoneh1iggspossessproliferativeactivitytowardshumantleukaemiacemcells AT magorivskai antihistoneh1iggspossessproliferativeactivitytowardshumantleukaemiacemcells AT bilyir antihistoneh1iggspossessproliferativeactivitytowardshumantleukaemiacemcells AT myronovskijs antihistoneh1iggspossessproliferativeactivitytowardshumantleukaemiacemcells AT stoikar antihistoneh1iggspossessproliferativeactivitytowardshumantleukaemiacemcells |
| first_indexed |
2025-12-07T18:10:29Z |
| last_indexed |
2025-12-07T18:10:29Z |
| _version_ |
1850874034657427456 |