Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells
The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammoni...
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| Published in: | Experimental Oncology |
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| Date: | 2017 |
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/137602 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862717396092977152 |
|---|---|
| author | Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. |
| author_facet | Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. |
| citation_txt | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens.
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| first_indexed | 2025-12-07T18:10:29Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-137602 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T18:10:29Z |
| publishDate | 2017 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. 2018-06-17T13:54:51Z 2018-06-17T13:54:51Z 2017 Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells / Yu. Kit1, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 36-41. — Бібліогр.: 34 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/137602 The aim of this study was to characterize the proliferative activity of the anti-histone H1 IgGs towards human T-leukaemia CEM cells. Materials and Methods: Anti-histone H1 IgGs were purified from blood serum of systemic lupus erythematosus patients by precipitation of serum proteins with 50% ammonium sulfate followed by a sequential affinity chromatography on Protein GSepharose and histone H1-Sepharose columns. To avoid contamination with other proteins, anti-histone H1 IgGs were subjected to strongly acidic pH 2.0 during gel filtration through HPLC column. The effects of the anti-histone H1 IgGs on cell viability and cell cycle were tested by MTS-assay and flow cytometry, correspondingly. The cross-reactivity of the anti-histone H1 antibodies towards heterogenetic and cellular antigens was evaluated by Western-blot analysis. Results: It was found that incubation of CEM cells with the HPLC-purified anti-histone H1 IgGs resulted in significant stimulation of cell growth by 46% after 48 h of incubation. These IgGs possess an antigenic poly-specificity to positively charged heterogenetic antigens and different cellular antigens. FITC-labeled and biotinylated anti-histone H1 IgGs are internalized by CEM cells and preferentially accumulated in the cytoplasm. Conclusion: The anti-histone H1 IgGs are shown to internalize human T-leukemia CEM and stimulate their proliferation. These IgGs are polyspecific toward cellular antigens. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells Article published earlier |
| spellingShingle | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells Kit, Yu. Magorivska, I. Bilyi, R. Myronovskij, S. Stoika, R. Original contributions |
| title | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_full | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_fullStr | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_full_unstemmed | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_short | Anti-histone H1 IgGs possess proliferative activity towards human T-leukaemia cem cells |
| title_sort | anti-histone h1 iggs possess proliferative activity towards human t-leukaemia cem cells |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/137602 |
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