Parthenolide reduces gene transcription of prosurvival mediators in U937 cells

Parthenolide reduces gene transcription of prosurvival mediators in U937 cells

In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-...

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Published in:Experimental Oncology
Date:2017
Main Authors: Mohammadi, S., Zahedpanah, M., Nikbakht, M., Shaiegan, M., Hamidollah Ghaffari, S., Nikugoftar, M., Rahmani, B., Hamedi Asl, D.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2017
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/137611
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-137611
record_format dspace
spelling Mohammadi, S.
Zahedpanah, M.
Nikbakht, M.
Shaiegan, M.
Hamidollah Ghaffari, S.
Nikugoftar, M.
Rahmani, B.
Hamedi Asl, D.
2018-06-17T14:01:40Z
2018-06-17T14:01:40Z
2017
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/137611
In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.
All authors claim no conflicts of interest or financial involvements with this manuscript. This study was funded by Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and by Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
spellingShingle Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
Mohammadi, S.
Zahedpanah, M.
Nikbakht, M.
Shaiegan, M.
Hamidollah Ghaffari, S.
Nikugoftar, M.
Rahmani, B.
Hamedi Asl, D.
Original contributions
title_short Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
title_full Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
title_fullStr Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
title_full_unstemmed Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
title_sort parthenolide reduces gene transcription of prosurvival mediators in u937 cells
author Mohammadi, S.
Zahedpanah, M.
Nikbakht, M.
Shaiegan, M.
Hamidollah Ghaffari, S.
Nikugoftar, M.
Rahmani, B.
Hamedi Asl, D.
author_facet Mohammadi, S.
Zahedpanah, M.
Nikbakht, M.
Shaiegan, M.
Hamidollah Ghaffari, S.
Nikugoftar, M.
Rahmani, B.
Hamedi Asl, D.
topic Original contributions
topic_facet Original contributions
publishDate 2017
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/137611
citation_txt Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ.
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first_indexed 2025-12-07T20:49:54Z
last_indexed 2025-12-07T20:49:54Z
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