Parthenolide reduces gene transcription of prosurvival mediators in U937 cells
In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-...
Збережено в:
| Опубліковано в: : | Experimental Oncology |
|---|---|
| Дата: | 2017 |
| Автори: | , , , , , , , |
| Формат: | Стаття |
| Мова: | Англійська |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
|
| Теми: | |
| Онлайн доступ: | https://nasplib.isofts.kiev.ua/handle/123456789/137611 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862747187843170304 |
|---|---|
| author | Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. |
| author_facet | Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. |
| citation_txt | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators.
|
| first_indexed | 2025-12-07T20:49:54Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-137611 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T20:49:54Z |
| publishDate | 2017 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. 2018-06-17T14:01:40Z 2018-06-17T14:01:40Z 2017 Parthenolide reduces gene transcription of prosurvival mediators in U937 cells / S. Mohammadi, M. Zahedpanah, M. Nikbakht, M. Shaiegan, S. Hamidollah Ghaffari, M. Nikugoftar, B. Rahmani, D. Hamedi Asl // Experimental Oncology. — 2017 — Т. 39, № 1. — С. 30-35. — Бібліогр.: 55 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/137611 In acute myeloid leukemia (AML) the functional abnormalities of osteopontin (OPN), NF-kB, PI3K/AKT/mTOR/PTEN pathway or β-catenin have been considered. Aim: To analyze the response of U937 cells to parthenolide (PTL) through the involvement of expression of OPN protein, RelB, AKT1, mTOR, PTEN and β-catenin genes. Materials and Methods: The U937 cells were treated with PTL at concentrations of 4 μM (IC25) or 6 μM (IC50) and with OPN siRNA for MTT assay and colony forming assay. Western blot analysis using antibodies against OPN was performed with lysates of PTL-treated cells. Quantitative real-time polymerase chain reaction was performed using primers for OPN siRNA, RelB, AKT1, mTOR, PTEN and β-catenin. Results: PTL reduces OPN protein level and down-regulates RelB mRNA in U937 cell line. Suppression of OPN with siRNA increases the cytotoxic effects of PTL. Also, mRNA expression of AKT1, mTOR, PTEN, and β-catenin decreases with PTL or OPN siRNA. Conclusion: Sensitivity of U937 cells to PTL can be associated with the reduction in expression of prosurvival mediators. All authors claim no conflicts of interest or financial involvements with this manuscript. This study was funded by Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine and by Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Parthenolide reduces gene transcription of prosurvival mediators in U937 cells Article published earlier |
| spellingShingle | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells Mohammadi, S. Zahedpanah, M. Nikbakht, M. Shaiegan, M. Hamidollah Ghaffari, S. Nikugoftar, M. Rahmani, B. Hamedi Asl, D. Original contributions |
| title | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_full | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_fullStr | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_full_unstemmed | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_short | Parthenolide reduces gene transcription of prosurvival mediators in U937 cells |
| title_sort | parthenolide reduces gene transcription of prosurvival mediators in u937 cells |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/137611 |
| work_keys_str_mv | AT mohammadis parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT zahedpanahm parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT nikbakhtm parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT shaieganm parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT hamidollahghaffaris parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT nikugoftarm parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT rahmanib parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells AT hamediasld parthenolidereducesgenetranscriptionofprosurvivalmediatorsinu937cells |