Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa
Aim: To analyze the glycoprotein binding sites of the gastric mucosa and its secreted mucus using lectin histochemistry in patients with chronic non-atrophic gastritis (CNAG) associated or not-associated with Helicobacter pylori infection with or without dysplasia. Materials and Methods: In order to...
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| Published in: | Experimental Oncology |
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| Date: | 2017 |
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/137631 |
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| Journal Title: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Cite this: | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa / S. Vernygorodskyi, V. Shkolnikov, D. Suhan // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 138–140. — Бібліогр.: 7 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862671278607958016 |
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| author | Vernygorodskyi, S. Shkolnikov, V. Suhan, D. |
| author_facet | Vernygorodskyi, S. Shkolnikov, V. Suhan, D. |
| citation_txt | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa / S. Vernygorodskyi, V. Shkolnikov, D. Suhan // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 138–140. — Бібліогр.: 7 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To analyze the glycoprotein binding sites of the gastric mucosa and its secreted mucus using lectin histochemistry in patients with chronic non-atrophic gastritis (CNAG) associated or not-associated with Helicobacter pylori infection with or without dysplasia. Materials and Methods: In order to identify the areas with glycoconjugates expression in gastric mucosa, 6 lectins (Canavalia ensiformis agglutinin — Con A, Sambucus nigra agglutinin — SNA, wheat germ agglutinin — WGA, soybean agglutinin — SBA, Helix pomatia agglutinin — HPA, peanut agglutinin — PNA) were used. Carbohydrate determinants were visualized according to the lectin-peroxidase-diaminobenzidine staining protocol. Biopsy material was obtained and processed by conventional histological methods. The samples from 84 patients (54 with CNAG) with low (n = 34) and high grade (n = 20) dysplasia, 38 patients were H. pylori-infected and 26 patients — H. pylori-noninfected) were used. The comparison group included 30 persons with CNAG without dysplasia (16 patients H. pylori-infected and 14 — noninfected). Results: In comparison to normal gastric mucosa, a low affinity of Con A was shown in 80% of patients with non-infected CNAG and 90% of H. pylori associated CNAG. In 70% of H. pylori-infected patients with CNAG and low grade dysplasia there was an increase of SNA expression compared with noninfected patients (p < 0.05). Regarding SBA labeling no differences were detected in the studied groups (p < 0.05). In H. pylori infected patients with CNAG and low grade dysplasia, WGA, HPA and PNA showed a strong reactivity with the gastric mucosa cells in 80; 75%, and 60% of patients, respectively. Conclusion: We suggest that a set of lectins in reaction with gastric epithelial and glandular cells can be used as a tool to obtain information about the dysplastic changes of the gastric mucosa and may offer new insight into gastric carcinogenesis and precancerous lesions treatment.
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| first_indexed | 2025-12-07T15:33:37Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-137631 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T15:33:37Z |
| publishDate | 2017 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Vernygorodskyi, S. Shkolnikov, V. Suhan, D. 2018-06-17T14:22:38Z 2018-06-17T14:22:38Z 2017 Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa / S. Vernygorodskyi, V. Shkolnikov, D. Suhan // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 138–140. — Бібліогр.: 7 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/137631 Aim: To analyze the glycoprotein binding sites of the gastric mucosa and its secreted mucus using lectin histochemistry in patients with chronic non-atrophic gastritis (CNAG) associated or not-associated with Helicobacter pylori infection with or without dysplasia. Materials and Methods: In order to identify the areas with glycoconjugates expression in gastric mucosa, 6 lectins (Canavalia ensiformis agglutinin — Con A, Sambucus nigra agglutinin — SNA, wheat germ agglutinin — WGA, soybean agglutinin — SBA, Helix pomatia agglutinin — HPA, peanut agglutinin — PNA) were used. Carbohydrate determinants were visualized according to the lectin-peroxidase-diaminobenzidine staining protocol. Biopsy material was obtained and processed by conventional histological methods. The samples from 84 patients (54 with CNAG) with low (n = 34) and high grade (n = 20) dysplasia, 38 patients were H. pylori-infected and 26 patients — H. pylori-noninfected) were used. The comparison group included 30 persons with CNAG without dysplasia (16 patients H. pylori-infected and 14 — noninfected). Results: In comparison to normal gastric mucosa, a low affinity of Con A was shown in 80% of patients with non-infected CNAG and 90% of H. pylori associated CNAG. In 70% of H. pylori-infected patients with CNAG and low grade dysplasia there was an increase of SNA expression compared with noninfected patients (p < 0.05). Regarding SBA labeling no differences were detected in the studied groups (p < 0.05). In H. pylori infected patients with CNAG and low grade dysplasia, WGA, HPA and PNA showed a strong reactivity with the gastric mucosa cells in 80; 75%, and 60% of patients, respectively. Conclusion: We suggest that a set of lectins in reaction with gastric epithelial and glandular cells can be used as a tool to obtain information about the dysplastic changes of the gastric mucosa and may offer new insight into gastric carcinogenesis and precancerous lesions treatment. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa Article published earlier |
| spellingShingle | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa Vernygorodskyi, S. Shkolnikov, V. Suhan, D. Original contributions |
| title | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_full | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_fullStr | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_full_unstemmed | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_short | Lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| title_sort | lectin binding patterns in normal, dysplastic and helicobacter pylori infected gastric mucosa |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/137631 |
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