High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels
Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77...
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2006
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| Cite this: | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels / A.P. Burlaka, E.P. Sidorik, I.I. Ganusevich, Yu.M. Lestchenko, A.A. Burlaka, S.P. Osinsky // Experimental Oncology. — 2006. — Т. 28, № 4. — С. 323-325. — Бібліогр.: 13 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1859913402949828608 |
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| author | Burlaka, A.P. Sidorik, E.P. Ganusevich, I.I. Lestchenko, Yu.M. Burlaka, A.А. Osinsky, S.P. |
| author_facet | Burlaka, A.P. Sidorik, E.P. Ganusevich, I.I. Lestchenko, Yu.M. Burlaka, A.А. Osinsky, S.P. |
| citation_txt | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels / A.P. Burlaka, E.P. Sidorik, I.I. Ganusevich, Yu.M. Lestchenko, A.A. Burlaka, S.P. Osinsky // Experimental Oncology. — 2006. — Т. 28, № 4. — С. 323-325. — Бібліогр.: 13 назв. — англ. |
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| description | Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77 °K, Spin Traps technology and zymography in polyacrylamide gels were applied. Results: In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO· and formation of complexes of NO· with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO· generation and formation of complexes of NO·· with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed. Conclusion: Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs.
Цель: изучить взаимоотношение между уровнями образования активных форм кислорода (АФК) и оксида азота (ОА) и активности
матриксных металлопротеиназ (MMPs) MMP-2 и MMP-9 в сосудах, изолированных из опухолевой ткани прямой кишки, и
в Arteria rectalis superior. Методы: ЭПР при комнатной температуре и при 77 °K, технология спиновых ловушек, зимофрафия в
полиакриламидном геле. Результаты: в сосудах, изолированных из опухолевой ткани прямой кишки, и в Arteria rectalis superior
обнаружены высокие уровни образования АФК, ·
и комплексов ·
с FeS-белками в участках цепи
митохондрий. Определена также высокая активность MMP-2 и MMP-9 в стенке сосудов, находящихся в опухолевом узле.
Обнаружена прямая положительная корреляция между величиной образования ·
и образованием комплексов · с
белками, а также между образованием АФК и NO и активность MMPs. Выводы: измененное окислительное равновесие в
митохондриях клеток стенки сосудов, находящихся в опухолевой ткани, усиливает формирование в них клеточной гипоксии,
ее аутокаталитическое потенцирование, что сопровождается активацией в этих клетках MMPs
|
| first_indexed | 2025-12-07T16:03:16Z |
| format | Article |
| fulltext |
Experimental Oncology ���� ��������� ����� ��ecem�er�� ������� ��������� ����� ��ecem�er�� ����ecem�er�� ����� ��� ���
It is known that ROS and NO may activate directly
and/or via signaling molecules the expression of
a num�er of genes controlling the function of the
vessels �oth at normal and pathologic states [1].
The angiogenesis is tightly linked to the development
of tumor and malignant progression�� in particular
metastasis. It is clearly shown that tumor invasion
as well as metastasis is accompanied with intra- and
extravasation�� and destruction of extracellular matrix
�y matrix metalloproteinases �MMPs���� in particular��
MMP-� and MMP-9 [��� �]. There is evidence that
degradation and reorganization of the extracellular
matrix are key events in vascular remodeling [4]. It was
determined that �oth pro-MMP-� and pro�MMP-9
secreted from human vascular smooth muscle cells
are activated �y ROS [�]. The activations of MMP-�
and MMP-9 in human gastric cancer �y ROS as well
as NO were o�served [��]. Taking into account the
a�ove mentioned data this study was aimed to detect
the levels of generation of ROS and NO and activities
of MMP-� and MMP-9 in vessels isolated from rectal
tumor and adjacent normal tissue.
Twenty seven patients undergoing surgical resection
of histologically proven rectal cancer ��N1��� grade
G��� were included into the trial. The patients were
not treated previously. All patients underwent poten-
tially curative resections. The vessels were o�tained �y
means of preparation of freshly harvested tumor tissue
with anatomical tools under magnification that allows
to yield at least 1�� mg of vascular materials. In addi-
tion�� a. rectalis superior feeding the tumor node in the
rectum was o�tained �y the resection of superampullar
part of rectum on the distance of 4�� cm from tumor
�oundary. The weight of o�tained vascular specimen
was approximately ��� mg. Moreover�� the �ranches
of a. gastroepiploica sinistra �at least 1�� mg�� were
o�tained �y the operation of patients with gastric ulcer
�n = 7��. Local ethics committee approval was o�tained
and all patients gave informed consent.
O�tained vascular tissues were snap frozen in
liquid nitrogen at the time of operation and stored at
�7� °C. The superoxide radical-anions formation was
assessed �y the method of EPR with the use of 1-hy-
droxy-������������-tetramethyl-4-oxopiperidin �Russia��
and Spin Traps technology. The rates of superoxide
radical-anions generation were calculated in nmol/g
fresh tissue per min. The level of NO was determined
using diethyldithiocar�omate spin trap �Sigma�� USA��
and EPR technology. The generation of NO was
registered during � min followed �y cessation of NO
generation �y liquid nitrogen �77 K��. The levels of NO
were calculated in nmol/g fresh tissue.
Activities of MMP-� and MMP-9 were determined
�y zymography in 1�% polyacrylamide gel with the ad-
dition of �.1% of gelatin as su�strate and using MMP-�
and MMP-9 standards �Sigma�� USA�� [7].
The results were statistically evaluated �y computer
programs Statistics ��.� and Exel ���� using paramet-
ric and correlation analysis.
In the vessels isolated from tumor tissue and in
a. rectalis superior�� there have �een revealed elevated
levels of generation rate of superoxide radical-anions
compared to those in �ranches of a. gastroepiploica
sinistra �Fig. 1��. Both in the vascular tissue isolated
from tumors and a. rectalis superior�� activation of
NO· synthesis �y NO-synthase systems�� in particu-
lar�� mNOS has �een revealed. In the tumor vessels��
NO· levels were at the range of �.7� nmol/g of fresh
tissue�� whilst in a. rectalis superior NO· level yielded
�.�1 nmol/g of fresh tissue �Fig. ���. These indices are
significantly higher than those in a. gastroepiploica
sinistra �Fig. ���. It has to �e mentioned that significant
HIGH FORMATION OF SUPEROXIDE ANION AND NITRIC OXIDE,
AND MATRIX METALLOPROTEINASES ACTIVITY IN VASCULAR
WALL OF RECTAL CARCINOMA VESSELS
A.P. Burlaka1, E.P. Sidorik1, I.I. Ganusevich1, *, Yu.M. Lestchenko3, A.A. Burlaka2, S.P. Osinsky1
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
2O.O. Bogomolets National Medical University, MH of Ukraine, Kyiv, Ukraine
3L.P. Shupik Kyiv Medical Academy of Postgraduate Education, MH of Ukraine, Kyiv, Ukraine
Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix
metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR
at the room temperature and 77 °K, Spin Traps technology and zymography in polyacrylamide gels were applied. Results: In the vessels iso-
lated from rectal tumors and Arteria rectalis superior high levels of ROS, NO· and formation of complexes of NO· with FeS-proteins at the
sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also
observed. The direct positive correlation between the rate of NO· generation and formation of complexes of NO·· with FeS-proteins as well
as between ROS and NO formation and MMPs activities have been revealed. Conclusion: Altered oxidative equilibrium in mitochondria of
cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs.
Key Words: vascular wall, radical oxygen species, nitric oxide, matrix metalloproteinases-2 and -9.
Received: November 21, 2006.
*Correspondence: E-mail: osion@onconet.kiev.ua
Abbreviations used: NO — nitric oxide; ROS - radical oxygen species.
Exp Oncol �����
���� 4�� �������
SHORT COMMUNICATIONS
��4 Experimental Oncology ���� ��������� ����� ��ecem�er��
differences �etween the rate of superoxide radical-
anions generation and NO levels in vessels feeding
the region of gastric wall with ulcer lesion and those
in vessels feeding the tumor tissue were determined.
The specificity of increased formation of reactive oxy-
gen and nitrogen species in tumor vessels is required
to �e confirmed in the special investigation with the
extended spectra of vascular specimens.
Fig. 1. The rate of superoxide radical-anions generation in the
vessels isolated from tumors �1�� and A. rectalis superior �2��
compared with such in vessels isolated from stomach wall in
patient with gastric ulcer �3��
Fig. 2. The levels of NO in the vessels isolated from tumors �1��
and Arteria rectalis superior �2�� compared with such in vessels
isolated from stomach wall in patient with gastric ulcer �3��
Intensity of EPR signal in the region of the factor of
spectroscopic splitting�� g = �.���� on the EPR spectra
of the vessels isolated from tumors differentiated from
such o�tained with a. rectalis superior �Fig. ���.
Fig. 3. EPR spectra of vessels isolated from tumor of rectum
�1���� and Arteria rectalis superior �2��
*g = �.�� characterizes the level of generated NO·-FeS-proteins
complexes in mitochondrial mem�ranes.
As we have reported earlier [�]�� the intensity of this
signal responds to the level of NO-FeS-protein com-
plexes generated upon interaction of NO· with FeS-pro-
teins of respiratory chain of mitochondrial mem�ranes��
in particular with the proteins from N and S clusters in
NA�PH-u�iquinone-oxidoreductase and succinatede
hydrogenase�� respectively. In the tumoral vessels�� high
content of NO-FeS-protein complexes �1.� ± �.11 a. u��
compared to such ��.�� ± �.�� a. u�� in a. rectalis su-
perior�� was revealed �Fig. 4��. One should note that in
normal state such complexes are generated at physi-
ologic concentrations ��.1� ± �.�1 a. u�� for regulation
of the rate of electron flow through respiratory chain of
mitochondria. High levels of the complexes of NO with
FeS-proteins in mitochondria are considered as indexes
of oxidative reactivity of the cells further expressed �y
NO effects in the formation of cell hypoxia and tumor
progression. Mechanism of such distur�ance upon
chemical carcinogens and during tumor growth was
descri�ed in our earlier investigations [���� 9].
Fig. 4. �ependence �etween the level of generation of NO·-
FeS-proteins complexes at the sites of respiratory chain of
mitochondria of cellular elements of the tumoral vessels and
concentration of NO· synthesized in the vascular wall of rectal
tumor vessels
Also�� we have revealed the direct positive relation
�etween generation of NO· complexes with FeS-pro-
teins at the sites of respiratory chain of mitochondria in
the vessels of rectal tumors and concentration of NO·
produced in the tumor �r = �.�4; p < �.�1��.
In the vessels isolated from tumor tissues and in
a. rectalis superior high levels of MMP-� and MMP-9
activities were o�served �Fig. ����� especially of the last
one that is considered characteristic for vascular
endothelium and play a pivotal role in the processes
related to the formation and function of vessels �oth
at normal and pathological states. It is known that
physiologic state of the vessels is characterized �y
very low �up to zero�� activities of MMP-� and MMP-9.
Upon malignant progression activities of MMP-� and
MMP-9 are increased making the conditions for intra-
and extravasation of tumor cells �y the destruction of
extracellular matrix that can results in the promotion
of tumor invasion and metastasis [��� �].
In the vessels of tumor and a. rectalis superior
the direct positive correlations �etween the levels of
ROS and NO· generation and activities of MMP-� and
MMP-9 have �een revealed �r = �.��; r = �.4�; r = �.47;
r = �.���; p < �.���� respectively�� that characterizes the
regulatory influence of ROS and NO· toward gelatina-
ses via activation of latent forms of the enzymes [��].
Experimental Oncology ���� ��������� ����� ��ecem�er�� ������� ��������� ����� ��ecem�er�� ����ecem�er�� ����� ��� ���
Fig. 5. Activities of matrix metalloproteinases in the vessels
isolated from tumor �1�� and in a. rectialis superior �2��
It is known that superoxide radical-anions produced
�y endothelial and vascular smooth muscle cells may
activate specific signal transduction pathways and
coordinate interrelated pathologic reactions in the
vascular wall [1�]. There are the data showing that the
process of extravasation is potentiated simultaneously
with an increase of NO· level generated �y human
НТ1��� fi�rosarcoma cells [11].
In conclusion�� taking into account the a�ove men-
tioned data and the results of other authors [��� 1���1�]
it may �e supposed that high levels of ROS and NO
generated in the vascular wall participate in the forma-
tion of cellular hypoxia and activation of matrix metal-
loproteinases in the cellular elements of tumor vessels.
These events may contri�ute to the metastatic process
simultaneously with those o�served in tumor cells.
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Высокий уроВень образоВания супероксидного
радикал-аниона и оксида азота и актиВности
матриксных металлопротеиназ В стенке сосудоВ
опухолей прямой кишки
Цель: изучить взаимоотношение между уровнями образования активных форм кислорода (АФК) и оксида азота (ОА) и активности
матриксных металлопротеиназ (MMPs) MMP-2 и MMP-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, иMMPs) MMP-2 и MMP-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, и) MMP-2 и MMP-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, иMMP-2 и MMP-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, и-2 и MMP-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, иMMP-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, и-9 в сосудах, изолированных из опухолево�� ткани прямо�� кишки, и
в Arteria rectalis superior. Методы: ЭПР при комнатно�� температуре и при 77 °K, техноло�ия спиновых ловушек, зимо�рафия вK, техноло�ия спиновых ловушек, зимо�рафия в, техноло�ия спиновых ловушек, зимо�рафия в
полиакриламидном �еле. Результаты: в сосудах, изолированных из опухолево�� ткани прямо�� кишки, и в Arteria rectalis superior
обнаружены высокие уровни образования АФК, NONO· и комплексов NONO· с FeS-белками в участках �лектронтранспортно�� �епис FeS-белками в участках �лектронтранспортно�� �епиFeS-белками в участках �лектронтранспортно�� �епибелками в участках �лектронтранспортно�� �епи
митохондри��. Определена также высокая активность MMP-2 и MMP-9 в стенке сосудов, находя�ихся в опухолевом узле.MMP-2 и MMP-9 в стенке сосудов, находя�ихся в опухолевом узле.-2 и MMP-9 в стенке сосудов, находя�ихся в опухолевом узле.MMP-9 в стенке сосудов, находя�ихся в опухолевом узле.-9 в стенке сосудов, находя�ихся в опухолевом узле.
Обнаружена прямая положительная корреля�ия между величино�� образования NONO· и образованием комплексов NONO· с FeS-с FeS-FeS-
белками, а также между образованием АФК и NO и активность�� MMPs.NO и активность�� MMPs. и активность�� MMPs.MMPs. Выводы: измененное окислительное равновесие в
митохондриях клеток стенки сосудов, находя�ихся в опухолево�� ткани, усиливает формирование в них клеточно�� �ипоксии,
ее аутокаталитическое потен�ирование, что сопровождается актива�ие�� в �тих клетках MMPs.MMPs..
Ключевые слова: сосудистая стенка, активные формы кислорода, оксид азота, матриксные металлопротеиназы-2 и -9.
Copyright © Experimental Oncology, 2006
|
| id | nasplib_isofts_kiev_ua-123456789-137932 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T16:03:16Z |
| publishDate | 2006 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Burlaka, A.P. Sidorik, E.P. Ganusevich, I.I. Lestchenko, Yu.M. Burlaka, A.А. Osinsky, S.P. 2018-06-17T19:12:49Z 2018-06-17T19:12:49Z 2006 High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels / A.P. Burlaka, E.P. Sidorik, I.I. Ganusevich, Yu.M. Lestchenko, A.A. Burlaka, S.P. Osinsky // Experimental Oncology. — 2006. — Т. 28, № 4. — С. 323-325. — Бібліогр.: 13 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/137932 Aim: To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior. Methods: EPR at the room temperature and 77 °K, Spin Traps technology and zymography in polyacrylamide gels were applied. Results: In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO· and formation of complexes of NO· with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO· generation and formation of complexes of NO·· with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed. Conclusion: Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs. Цель: изучить взаимоотношение между уровнями образования активных форм кислорода (АФК) и оксида азота (ОА) и активности матриксных металлопротеиназ (MMPs) MMP-2 и MMP-9 в сосудах, изолированных из опухолевой ткани прямой кишки, и в Arteria rectalis superior. Методы: ЭПР при комнатной температуре и при 77 °K, технология спиновых ловушек, зимофрафия в полиакриламидном геле. Результаты: в сосудах, изолированных из опухолевой ткани прямой кишки, и в Arteria rectalis superior обнаружены высокие уровни образования АФК, · и комплексов · с FeS-белками в участках цепи митохондрий. Определена также высокая активность MMP-2 и MMP-9 в стенке сосудов, находящихся в опухолевом узле. Обнаружена прямая положительная корреляция между величиной образования · и образованием комплексов · с белками, а также между образованием АФК и NO и активность MMPs. Выводы: измененное окислительное равновесие в митохондриях клеток стенки сосудов, находящихся в опухолевой ткани, усиливает формирование в них клеточной гипоксии, ее аутокаталитическое потенцирование, что сопровождается активацией в этих клетках MMPs en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Short communications High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels высокий уровень образования супероксидного радикал-аниона и оксида азота и активности матриксных металлопротеиназ в стенке сосудов опухолей прямой кишки Article published earlier |
| spellingShingle | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels Burlaka, A.P. Sidorik, E.P. Ganusevich, I.I. Lestchenko, Yu.M. Burlaka, A.А. Osinsky, S.P. Short communications |
| title | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels |
| title_alt | высокий уровень образования супероксидного радикал-аниона и оксида азота и активности матриксных металлопротеиназ в стенке сосудов опухолей прямой кишки |
| title_full | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels |
| title_fullStr | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels |
| title_full_unstemmed | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels |
| title_short | High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels |
| title_sort | high formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels |
| topic | Short communications |
| topic_facet | Short communications |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/137932 |
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