Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma
Aim: The genetic mechanisms of resistance to chemotherapy in B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) are not clear. We aimed to determine the peculiarities of abnormal karyotype formation in bone marrow (BM) cells and peripheral blood (PB) blast transformed B-cells...
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| Published in: | Experimental Oncology |
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| Date: | 2017 |
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| Format: | Article |
| Language: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Online Access: | https://nasplib.isofts.kiev.ua/handle/123456789/137974 |
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| Cite this: | Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma / S.V. Andreieva, K.V. Korets, O.E. Ruzhinska, I.M. Skorokhod , O.G. Alkhimova // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 141–144. — Бібліогр.: 20 назв. — англ. |
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Andreieva, S.V. Korets, K.V. Ruzhinska, O.E. Skorokhod, I.M. Alkhimova, O.G. 2018-06-17T20:05:37Z 2018-06-17T20:05:37Z 2017 Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma / S.V. Andreieva, K.V. Korets, O.E. Ruzhinska, I.M. Skorokhod , O.G. Alkhimova // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 141–144. — Бібліогр.: 20 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/137974 Aim: The genetic mechanisms of resistance to chemotherapy in B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) are not clear. We aimed to determine the peculiarities of abnormal karyotype formation in bone marrow (BM) cells and peripheral blood (PB) blast transformed B-cells in relapse of B-CLL/SLL. Materials and Methods: Cytogenetic GTG banding technique and molecular cytogenetic in interphase cells (i-FISH) studies of BM cells and PB blast transformed B-lymphocytes were performed in 14 patients (10 males and 4 females) with B-CLL/SLL. Results: The results of karyotyping BM and PB cells revealed the heterogeneity of cytogenetic abnormalities in combined single nosological group of B-CLL/SLL. In PB B-cells, chromosome abnormalities related to a poor prognosis group were registered 2.5 times more often than in BM cells. Additional near tetraploid clones that occurred in 57.1% cases were the peculiar feature of BM cell karyotypes. Chromosomal rearrangements characteristic of the group of adverse cytogenetic prognosis were revealed in all cases from which in 2 cases by karyotyping BM cells, in 6 cases in PB B-cells and in 8 cases by the i-FISH method in BM cells, i.e. their detection frequency was 3 times higher in PB B-cells and 4 times higher when analyzing by i-FISH in BM cells. Conclusions: Mismatch in abnormal karyotypes in BM and PB B-cells by the presence of quantitative and structural chromosomal rearrangements may be indicative of simultaneous and independent processes of abnormal clone formation in the lymph nodes and BM hematopoietic cells. Accumulation the information about previously unidentified chromosomal rearrangements in relapse of the disease may help to understand the ways of resistance formation to chemotherapy. We thank Prof. Danylo Gluzman, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NAS of Ukraine, for the confirmation of diagnosis and stage of the disease. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma Article published earlier |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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| title |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma |
| spellingShingle |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma Andreieva, S.V. Korets, K.V. Ruzhinska, O.E. Skorokhod, I.M. Alkhimova, O.G. Original contributions |
| title_short |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma |
| title_full |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma |
| title_fullStr |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma |
| title_full_unstemmed |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma |
| title_sort |
comparison of chromosomal rearrangements in bone marrow cells and blast transformed b-cells in relapse of b-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma |
| author |
Andreieva, S.V. Korets, K.V. Ruzhinska, O.E. Skorokhod, I.M. Alkhimova, O.G. |
| author_facet |
Andreieva, S.V. Korets, K.V. Ruzhinska, O.E. Skorokhod, I.M. Alkhimova, O.G. |
| topic |
Original contributions |
| topic_facet |
Original contributions |
| publishDate |
2017 |
| language |
English |
| container_title |
Experimental Oncology |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| format |
Article |
| description |
Aim: The genetic mechanisms of resistance to chemotherapy in B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) are not clear. We aimed to determine the peculiarities of abnormal karyotype formation in bone marrow (BM) cells and peripheral blood (PB) blast transformed B-cells in relapse of B-CLL/SLL. Materials and Methods: Cytogenetic GTG banding technique and molecular cytogenetic in interphase cells (i-FISH) studies of BM cells and PB blast transformed B-lymphocytes were performed in 14 patients (10 males and 4 females) with B-CLL/SLL. Results: The results of karyotyping BM and PB cells revealed the heterogeneity of cytogenetic abnormalities in combined single nosological group of B-CLL/SLL. In PB B-cells, chromosome abnormalities related to a poor prognosis group were registered 2.5 times more often than in BM cells. Additional near tetraploid clones that occurred in 57.1% cases were the peculiar feature of BM cell karyotypes. Chromosomal rearrangements characteristic of the group of adverse cytogenetic prognosis were revealed in all cases from which in 2 cases by karyotyping BM cells, in 6 cases in PB B-cells and in 8 cases by the i-FISH method in BM cells, i.e. their detection frequency was 3 times higher in PB B-cells and 4 times higher when analyzing by i-FISH in BM cells. Conclusions: Mismatch in abnormal karyotypes in BM and PB B-cells by the presence of quantitative and structural chromosomal rearrangements may be indicative of simultaneous and independent processes of abnormal clone formation in the lymph nodes and BM hematopoietic cells. Accumulation the information about previously unidentified chromosomal rearrangements in relapse of the disease may help to understand the ways of resistance formation to chemotherapy.
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| issn |
1812-9269 |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/137974 |
| citation_txt |
Comparison of chromosomal rearrangements in bone marrow cells and blast transformed B-cells in relapse of B-cell chronic lymphocytic leukemia/ small lymphocytic lymphoma / S.V. Andreieva, K.V. Korets, O.E. Ruzhinska, I.M. Skorokhod , O.G. Alkhimova // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 141–144. — Бібліогр.: 20 назв. — англ. |
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| first_indexed |
2025-12-07T17:03:48Z |
| last_indexed |
2025-12-07T17:03:48Z |
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