Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro

Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro

Aim: To investigate the mechanisms of cytotoxic activity and pro-/antioxidant effect of lactoferrin on hormone receptor-positive and receptor-negative breast cancer cells in vitro. Materials and Methods: The study was performed on receptor-positive (MCF-7, T47D) and receptor-negative (MDA-MB-231, MD...

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Veröffentlicht in:Experimental Oncology
Datum:2017
Hauptverfasser: Zalutski, I.V., Lukianova, N.Y., Storchai, D.M., Burlaka, A.P., Shvets, Y.V., Borikun, T.V., Todor, I.M., Lukashevich, V.S., Rudnichenko, Y.A., Chekhun, V.F.
Format: Artikel
Sprache:English
Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2017
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Online Zugang:https://nasplib.isofts.kiev.ua/handle/123456789/137977
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Zitieren:Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro / I.V. Zalutski, N.Y. Lukianova, D.M. Storchai, A.P. Burlaka, Y.V. Shvets, T.V. Borikun, I.M. Todor, V.S. Lukashevich, Y.A. Rudnichenko, V.F. Chekhun // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 106–111. — Бібліогр.: 21 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-137977
record_format dspace
spelling Zalutski, I.V.
Lukianova, N.Y.
Storchai, D.M.
Burlaka, A.P.
Shvets, Y.V.
Borikun, T.V.
Todor, I.M.
Lukashevich, V.S.
Rudnichenko, Y.A.
Chekhun, V.F.
2018-06-17T20:07:53Z
2018-06-17T20:07:53Z
2017
Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro / I.V. Zalutski, N.Y. Lukianova, D.M. Storchai, A.P. Burlaka, Y.V. Shvets, T.V. Borikun, I.M. Todor, V.S. Lukashevich, Y.A. Rudnichenko, V.F. Chekhun // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 106–111. — Бібліогр.: 21 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/137977
Aim: To investigate the mechanisms of cytotoxic activity and pro-/antioxidant effect of lactoferrin on hormone receptor-positive and receptor-negative breast cancer cells in vitro. Materials and Methods: The study was performed on receptor-positive (MCF-7, T47D) and receptor-negative (MDA-MB-231, MDA-MB-468) human breast cancer cell lines. Immunocytochemical staining, flow cytometry, low-temperature electron paramagnetic resonance, and the Comet assay were used. Results: Upon treatment with lactoferrin, the increased levels of reactive oxygen species (ROS) (p < 0.05), NO generation rate by inducible NO-synthase (p < 0.05) and the level of “free” iron (p < 0.05) were observed. Moreover, the effects of lactoferrin were more pronounced in receptor-negative MDA-MB-231 and MDA-MB-468 cells. These changes resulted in increased expression of proapoptotic Bax protein (p < 0.05), reduced expression of the antiapoptotic Bcl-2 protein (p < 0.05) and level of not-oxidized mitochondrial cardiolipin (1.4–1.7-fold, p < 0.05). This, in turn, caused an increase in the percentage of apoptotic cells (by 14–24%, p < 0.05). Cytotoxic effects of lactoferrin were accompanied by an increase in the percentage of DNA in the comet tail and blocking cell cycle at G₂/M phase, especially in receptor-negative cell lines. Conclusion: The study showed that exogenous lactoferrin causes a violation of an antioxidant balance by increasing the level of ROS, “free” iron and NO generation rate, resalting in the blocking of cell cycle at G₂/M-phase and apoptosis of malignant cells.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
spellingShingle Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
Zalutski, I.V.
Lukianova, N.Y.
Storchai, D.M.
Burlaka, A.P.
Shvets, Y.V.
Borikun, T.V.
Todor, I.M.
Lukashevich, V.S.
Rudnichenko, Y.A.
Chekhun, V.F.
Original contributions
title_short Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
title_full Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
title_fullStr Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
title_full_unstemmed Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
title_sort influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro
author Zalutski, I.V.
Lukianova, N.Y.
Storchai, D.M.
Burlaka, A.P.
Shvets, Y.V.
Borikun, T.V.
Todor, I.M.
Lukashevich, V.S.
Rudnichenko, Y.A.
Chekhun, V.F.
author_facet Zalutski, I.V.
Lukianova, N.Y.
Storchai, D.M.
Burlaka, A.P.
Shvets, Y.V.
Borikun, T.V.
Todor, I.M.
Lukashevich, V.S.
Rudnichenko, Y.A.
Chekhun, V.F.
topic Original contributions
topic_facet Original contributions
publishDate 2017
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: To investigate the mechanisms of cytotoxic activity and pro-/antioxidant effect of lactoferrin on hormone receptor-positive and receptor-negative breast cancer cells in vitro. Materials and Methods: The study was performed on receptor-positive (MCF-7, T47D) and receptor-negative (MDA-MB-231, MDA-MB-468) human breast cancer cell lines. Immunocytochemical staining, flow cytometry, low-temperature electron paramagnetic resonance, and the Comet assay were used. Results: Upon treatment with lactoferrin, the increased levels of reactive oxygen species (ROS) (p < 0.05), NO generation rate by inducible NO-synthase (p < 0.05) and the level of “free” iron (p < 0.05) were observed. Moreover, the effects of lactoferrin were more pronounced in receptor-negative MDA-MB-231 and MDA-MB-468 cells. These changes resulted in increased expression of proapoptotic Bax protein (p < 0.05), reduced expression of the antiapoptotic Bcl-2 protein (p < 0.05) and level of not-oxidized mitochondrial cardiolipin (1.4–1.7-fold, p < 0.05). This, in turn, caused an increase in the percentage of apoptotic cells (by 14–24%, p < 0.05). Cytotoxic effects of lactoferrin were accompanied by an increase in the percentage of DNA in the comet tail and blocking cell cycle at G₂/M phase, especially in receptor-negative cell lines. Conclusion: The study showed that exogenous lactoferrin causes a violation of an antioxidant balance by increasing the level of ROS, “free” iron and NO generation rate, resalting in the blocking of cell cycle at G₂/M-phase and apoptosis of malignant cells.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/137977
citation_txt Influence of exogenous lactoferrin on the oxidant/ antioxidant balance and molecular profile of hormone receptor-positive and -negative human breast cancer cells in vitro / I.V. Zalutski, N.Y. Lukianova, D.M. Storchai, A.P. Burlaka, Y.V. Shvets, T.V. Borikun, I.M. Todor, V.S. Lukashevich, Y.A. Rudnichenko, V.F. Chekhun // Experimental Oncology. — 2017 — Т. 39, № 2. — С. 106–111. — Бібліогр.: 21 назв. — англ.
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last_indexed 2025-12-07T16:16:54Z
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