A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice

Background: Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and extracellular matrix components. We previously demonstrated the effectiveness...

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Бібліографічні деталі
Опубліковано в: :Experimental Oncology
Дата:2016
Автори: Roomi, M.W., Kalinovsky, T., Rath, M., Niedzwiecki, A.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2016
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Онлайн доступ:https://nasplib.isofts.kiev.ua/handle/123456789/137980
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice / M.W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 54–56. — Бібліогр.: 9 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-137980
record_format dspace
spelling Roomi, M.W.
Kalinovsky, T.
Rath, M.
Niedzwiecki, A.
2018-06-17T20:12:12Z
2018-06-17T20:12:12Z
2016
A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice / M.W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 54–56. — Бібліогр.: 9 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/137980
Background: Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and extracellular matrix components. We previously demonstrated the effectiveness of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract in vitro: on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion. Aim: Our main objective in this study was to investigate the effect of the NM in vivo on human glioblastoma U-87 MG cell line. Materials and Methods: Athymic male nude mice inoculated with 3·106 U-87 MG cells subcutaneously and were fed a regular diet or a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed, the tumors were weighed and measured. The samples were studied histologically. Results: NM inhibited tumor weight and tumor burden by 53% (p = 0.015) and 48% (p = 0.010), respectively. Conclusions: These results suggest the therapeutic potential of NM as an adjuvant in the treatment of glioblastoma.
The research study was funded by Dr. Rath Health Foundation (Santa Clara, CA, USA), a non-profit organization. We thank consulting pathologist Alexander de Paoli, DVM, PhD, IDEXX Reference Laboratories for providing histopathology slides.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Short communications
A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
spellingShingle A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
Roomi, M.W.
Kalinovsky, T.
Rath, M.
Niedzwiecki, A.
Short communications
title_short A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
title_full A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
title_fullStr A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
title_full_unstemmed A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice
title_sort nutrient mixture inhibits glioblastoma xenograft u-87 mg growth in male nude mice
author Roomi, M.W.
Kalinovsky, T.
Rath, M.
Niedzwiecki, A.
author_facet Roomi, M.W.
Kalinovsky, T.
Rath, M.
Niedzwiecki, A.
topic Short communications
topic_facet Short communications
publishDate 2016
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Background: Brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and extracellular matrix components. We previously demonstrated the effectiveness of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract in vitro: on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion. Aim: Our main objective in this study was to investigate the effect of the NM in vivo on human glioblastoma U-87 MG cell line. Materials and Methods: Athymic male nude mice inoculated with 3·106 U-87 MG cells subcutaneously and were fed a regular diet or a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed, the tumors were weighed and measured. The samples were studied histologically. Results: NM inhibited tumor weight and tumor burden by 53% (p = 0.015) and 48% (p = 0.010), respectively. Conclusions: These results suggest the therapeutic potential of NM as an adjuvant in the treatment of glioblastoma.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/137980
citation_txt A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice / M.W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 54–56. — Бібліогр.: 9 назв. — англ.
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