Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis

Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis

The aim of the study was to improve cytogenetic diagnostics and monitoring of myelofibrosis and to reveal the spectrum of cytogenetic abnormalities in patients from Ukraine. Materials and Methods: A total of 42 patients (23 females and 19 males) with myelofibrosis was studied using different cytogen...

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Published in:Experimental Oncology
Date:2016
Main Authors: Lozynskyy, R.Y., Lozynska, M.R., Hontar, Y.V., Huleyuk, N.L., Maslyak, Z.V., Novak, V.L.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2016
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/137981
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis / R.Y. Lozynskyy, M.R. Lozynska, Y.V. Hontar, N.L. Huleyuk, Z.V. Maslyak, V.L. Novak // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 40–44. — Бібліогр.: 14 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-137981
record_format dspace
spelling Lozynskyy, R.Y.
Lozynska, M.R.
Hontar, Y.V.
Huleyuk, N.L.
Maslyak, Z.V.
Novak, V.L.
2018-06-17T20:13:19Z
2018-06-17T20:13:19Z
2016
Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis / R.Y. Lozynskyy, M.R. Lozynska, Y.V. Hontar, N.L. Huleyuk, Z.V. Maslyak, V.L. Novak // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 40–44. — Бібліогр.: 14 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/137981
The aim of the study was to improve cytogenetic diagnostics and monitoring of myelofibrosis and to reveal the spectrum of cytogenetic abnormalities in patients from Ukraine. Materials and Methods: A total of 42 patients (23 females and 19 males) with myelofibrosis was studied using different cytogenetic methods. Granulocyte colony-stimulating factor (G-CSF) was added by the new method during cultivation of peripheral blood (PB) cells from 31 patients for specific stimulation of mitotic divisions. Two patients underwent examination by fluorescent in situ hybridization method. Results: In cell cultures of PB stimulated in vitro with G-CSF and in non-stimulated bone marrow chromosome abnormalities were found in 19 (45.2%) of all the patients. The spectrum of cytogenetic abnormalities of bone marrow and PB was the same in all of the patients. Aspiration of bone marrow was unsuccessful due to significant fibrosis in 10 (29.4%) of 34 patients. The study by fluorescent in situ hybridization method confirmed cytogenetic abnormalities revealed by G-method and discovered additional possibly normal subclone. Conclusions: Cytogenetic study of PB using in vitro G-CSF as a specific stimulant of mitosis instead of phytohemagglutinin revealed significant variety of chromosomal abnormalities in Ukrainian patients with myelofibrosis. This method could be a less invasive alternative to cytogenetic examination of bone marrow in the subgroup of patients with considerable fibrosis and consecutive changes. The usage of fluorescent in situ hybridization method supplemented karyotyping by G-banding method.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
spellingShingle Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
Lozynskyy, R.Y.
Lozynska, M.R.
Hontar, Y.V.
Huleyuk, N.L.
Maslyak, Z.V.
Novak, V.L.
Original contributions
title_short Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
title_full Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
title_fullStr Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
title_full_unstemmed Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
title_sort study of cytogenetic abnormalities in g-csf stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis
author Lozynskyy, R.Y.
Lozynska, M.R.
Hontar, Y.V.
Huleyuk, N.L.
Maslyak, Z.V.
Novak, V.L.
author_facet Lozynskyy, R.Y.
Lozynska, M.R.
Hontar, Y.V.
Huleyuk, N.L.
Maslyak, Z.V.
Novak, V.L.
topic Original contributions
topic_facet Original contributions
publishDate 2016
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description The aim of the study was to improve cytogenetic diagnostics and monitoring of myelofibrosis and to reveal the spectrum of cytogenetic abnormalities in patients from Ukraine. Materials and Methods: A total of 42 patients (23 females and 19 males) with myelofibrosis was studied using different cytogenetic methods. Granulocyte colony-stimulating factor (G-CSF) was added by the new method during cultivation of peripheral blood (PB) cells from 31 patients for specific stimulation of mitotic divisions. Two patients underwent examination by fluorescent in situ hybridization method. Results: In cell cultures of PB stimulated in vitro with G-CSF and in non-stimulated bone marrow chromosome abnormalities were found in 19 (45.2%) of all the patients. The spectrum of cytogenetic abnormalities of bone marrow and PB was the same in all of the patients. Aspiration of bone marrow was unsuccessful due to significant fibrosis in 10 (29.4%) of 34 patients. The study by fluorescent in situ hybridization method confirmed cytogenetic abnormalities revealed by G-method and discovered additional possibly normal subclone. Conclusions: Cytogenetic study of PB using in vitro G-CSF as a specific stimulant of mitosis instead of phytohemagglutinin revealed significant variety of chromosomal abnormalities in Ukrainian patients with myelofibrosis. This method could be a less invasive alternative to cytogenetic examination of bone marrow in the subgroup of patients with considerable fibrosis and consecutive changes. The usage of fluorescent in situ hybridization method supplemented karyotyping by G-banding method.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/137981
citation_txt Study of cytogenetic abnormalities in G-CSF stimulated peripheral blood cells and non-stimulated bone marrow cells of patients with myelofibrosis / R.Y. Lozynskyy, M.R. Lozynska, Y.V. Hontar, N.L. Huleyuk, Z.V. Maslyak, V.L. Novak // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 40–44. — Бібліогр.: 14 назв. — англ.
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first_indexed 2025-12-07T13:32:38Z
last_indexed 2025-12-07T13:32:38Z
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