Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation

• Datum: 2016
• Hauptverfasser: Franskevych, D.V., Grynyuk, I.I., Prylutska, S.V., Pasichnyk, G.V., Petukhov, D.M., Drobot, L.B., Matyshevska, O.P., Ritter, U.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2016
• Schriftenreihe: Experimental Oncology
• Schlagworte: Original contributions
• Online Zugang: https://nasplib.isofts.kiev.ua/handle/123456789/138002
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Zitieren: Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation / D.V. Franskevych, I.I. Grynyuk, S.V. Prylutska, G.V. Pasichnyk, D.M. Petukhov, L.B. Drobot, O.P. Matyshevska, U. Ritter // Experimental Oncology. — 2016 — Т. 38, № 2. — С. 89–93. — Бібліогр.: 26 назв. — англ.

Aim: To estimate the combined action of C₆₀ fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lamp (λ = 410–700 nm, 2.45 J/cm²) was used for C₆₀ fullerene photoexcitation. Nitrite level and NO-synthase activity were measured by Griess reaction and by conversion of L-arginine to L-citrulline, respectively. p38 MAPK activity was assessed by Western blot analysis. Cell cycle distribution was determined by flow cytometry. Results: It was shown that light irradiation of C₆₀ fullerene-treated L1210 cells was accompanied by 55% decrease of their viability at 48 h of culture. Nitrite level measured as an index of reactive NO generation was increased at the early period after C₆₀ fullerene photoexcitation due to activation of both constitutive and inducible NO-synthase isoforms. The simultaneous activation of p38 MAPK was detected. Accumulation of L1210 cells in sub-G₁ phase of cell cycle was observed after C₆₀ fullerene photoexcitation. Conclusion: Photoexcited C₆₀ fullerene exerts cytotoxic effect, at least in part, through triggering production of reactive NO species and activation of p38 kinase apoptotic pathways in L1210 leukemic cells.