Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation
Aim: To estimate the combined action of C₆₀ fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lam...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2016 |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2016
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation / D.V. Franskevych, I.I. Grynyuk, S.V. Prylutska, G.V. Pasichnyk, D.M. Petukhov, L.B. Drobot, O.P. Matyshevska, U. Ritter // Experimental Oncology. — 2016 — Т. 38, № 2. — С. 89–93. — Бібліогр.: 26 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862545017016418304 |
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| author | Franskevych, D.V. Grynyuk, I.I. Prylutska, S.V. Pasichnyk, G.V. Petukhov, D.M. Drobot, L.B. Matyshevska, O.P. Ritter, U. |
| author_facet | Franskevych, D.V. Grynyuk, I.I. Prylutska, S.V. Pasichnyk, G.V. Petukhov, D.M. Drobot, L.B. Matyshevska, O.P. Ritter, U. |
| citation_txt | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation / D.V. Franskevych, I.I. Grynyuk, S.V. Prylutska, G.V. Pasichnyk, D.M. Petukhov, L.B. Drobot, O.P. Matyshevska, U. Ritter // Experimental Oncology. — 2016 — Т. 38, № 2. — С. 89–93. — Бібліогр.: 26 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To estimate the combined action of C₆₀ fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lamp (λ = 410–700 nm, 2.45 J/cm²) was used for C₆₀ fullerene photoexcitation. Nitrite level and NO-synthase activity were measured by Griess reaction and by conversion of L-arginine to L-citrulline, respectively. p38 MAPK activity was assessed by Western blot analysis. Cell cycle distribution was determined by flow cytometry. Results: It was shown that light irradiation of C₆₀ fullerene-treated L1210 cells was accompanied by 55% decrease of their viability at 48 h of culture. Nitrite level measured as an index of reactive NO generation was increased at the early period after C₆₀ fullerene photoexcitation due to activation of both constitutive and inducible NO-synthase isoforms. The simultaneous activation of p38 MAPK was detected. Accumulation of L1210 cells in sub-G₁ phase of cell cycle was observed after C₆₀ fullerene photoexcitation. Conclusion: Photoexcited C₆₀ fullerene exerts cytotoxic effect, at least in part, through triggering production of reactive NO species and activation of p38 kinase apoptotic pathways in L1210 leukemic cells.
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| first_indexed | 2025-11-25T04:38:18Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-138002 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-25T04:38:18Z |
| publishDate | 2016 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Franskevych, D.V. Grynyuk, I.I. Prylutska, S.V. Pasichnyk, G.V. Petukhov, D.M. Drobot, L.B. Matyshevska, O.P. Ritter, U. 2018-06-17T20:45:05Z 2018-06-17T20:45:05Z 2016 Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation / D.V. Franskevych, I.I. Grynyuk, S.V. Prylutska, G.V. Pasichnyk, D.M. Petukhov, L.B. Drobot, O.P. Matyshevska, U. Ritter // Experimental Oncology. — 2016 — Т. 38, № 2. — С. 89–93. — Бібліогр.: 26 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138002 Aim: To estimate the combined action of C₆₀ fullerene and light irradiation on viability of L1210 leukemic cells, nitric oxide (NO) generation, p38 mitogen-activated protein kinase (MAPK) activity and cell cycle distribution. Methods: Cell viability was assessed by MTT test. Light-emitting diode lamp (λ = 410–700 nm, 2.45 J/cm²) was used for C₆₀ fullerene photoexcitation. Nitrite level and NO-synthase activity were measured by Griess reaction and by conversion of L-arginine to L-citrulline, respectively. p38 MAPK activity was assessed by Western blot analysis. Cell cycle distribution was determined by flow cytometry. Results: It was shown that light irradiation of C₆₀ fullerene-treated L1210 cells was accompanied by 55% decrease of their viability at 48 h of culture. Nitrite level measured as an index of reactive NO generation was increased at the early period after C₆₀ fullerene photoexcitation due to activation of both constitutive and inducible NO-synthase isoforms. The simultaneous activation of p38 MAPK was detected. Accumulation of L1210 cells in sub-G₁ phase of cell cycle was observed after C₆₀ fullerene photoexcitation. Conclusion: Photoexcited C₆₀ fullerene exerts cytotoxic effect, at least in part, through triggering production of reactive NO species and activation of p38 kinase apoptotic pathways in L1210 leukemic cells. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation Article published earlier |
| spellingShingle | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation Franskevych, D.V. Grynyuk, I.I. Prylutska, S.V. Pasichnyk, G.V. Petukhov, D.M. Drobot, L.B. Matyshevska, O.P. Ritter, U. Original contributions |
| title | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation |
| title_full | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation |
| title_fullStr | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation |
| title_full_unstemmed | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation |
| title_short | Photocytotoxic effect of C₆₀ fullerene against L1210 leukemic cells is accomPanied by enhanced nitric oxide Production and p38 maPk activation |
| title_sort | photocytotoxic effect of c₆₀ fullerene against l1210 leukemic cells is accompanied by enhanced nitric oxide production and p38 mapk activation |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138002 |
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