DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients
Aim: To evaluate the generation and repair of DNA double strand breaks (DSBs) as a critical factors that define the efficiency of radiation therapy of cancer patients. Methods: Peripheral blood lymphocytes obtained from 18 patients with head and neck squamous cell carcinoma (HNSCC) and 18 healthy do...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2009 |
| Hauptverfasser: | , , , , , , , , |
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| Sprache: | Englisch |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/138137 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients / P. Rusin, L. Markiewicz, J. Olszewski, A. Morawiec-Sztandera, M. Kowalski, K. Pezybylowska, D. Kaczmarczyk, K. Kusmierczyk, I. Majstereksup // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 168-173. — Бібліогр.: 20 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862539411063832576 |
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| author | Rusin, P. Markiewicz, L. Olszewski, J. Morawiec-Sztandera, A. Kowalski, M. Przybylowska, K. Kaczmarczyk, D. Kusmierczyk, K. Majstereksup, I. |
| author_facet | Rusin, P. Markiewicz, L. Olszewski, J. Morawiec-Sztandera, A. Kowalski, M. Przybylowska, K. Kaczmarczyk, D. Kusmierczyk, K. Majstereksup, I. |
| citation_txt | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients / P. Rusin, L. Markiewicz, J. Olszewski, A. Morawiec-Sztandera, M. Kowalski, K. Pezybylowska, D. Kaczmarczyk, K. Kusmierczyk, I. Majstereksup // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 168-173. — Бібліогр.: 20 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: To evaluate the generation and repair of DNA double strand breaks (DSBs) as a critical factors that define the efficiency of radiation therapy of cancer patients. Methods: Peripheral blood lymphocytes obtained from 18 patients with head and neck squamous cell carcinoma (HNSCC) and 18 healthy donors were studied. The efficiency of DSBs repair after genotoxic treatment with hydrogen peroxide and γ-radiation were examined by neutral comet assay. MTT assay was used for cell viability analysis and Annexin V-FITC kit specific for kinase-3 was employed to determine apoptosis. Results: Lymphocytes from HNSCC patients were sensitive to genotoxic treatment and displayed impaired DSBs repair. Finally, as a consequence of this finding we have evidenced higher rate of apoptosis induction after γ-radiation treatment of lymphocytes from HNSCC patients than those from healthy controls. Conclusions: DSBs repair and increased apoptosis in cells of patients with head and neck cancer is relevant for efficient therapy of HNSCC.
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| first_indexed | 2025-11-24T15:15:10Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-138137 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-24T15:15:10Z |
| publishDate | 2009 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Rusin, P. Markiewicz, L. Olszewski, J. Morawiec-Sztandera, A. Kowalski, M. Przybylowska, K. Kaczmarczyk, D. Kusmierczyk, K. Majstereksup, I. 2018-06-18T09:41:22Z 2018-06-18T09:41:22Z 2009 DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients / P. Rusin, L. Markiewicz, J. Olszewski, A. Morawiec-Sztandera, M. Kowalski, K. Pezybylowska, D. Kaczmarczyk, K. Kusmierczyk, I. Majstereksup // Experimental Oncology. — 2009. — Т. 31, № 3. — С. 168-173. — Бібліогр.: 20 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138137 Aim: To evaluate the generation and repair of DNA double strand breaks (DSBs) as a critical factors that define the efficiency of radiation therapy of cancer patients. Methods: Peripheral blood lymphocytes obtained from 18 patients with head and neck squamous cell carcinoma (HNSCC) and 18 healthy donors were studied. The efficiency of DSBs repair after genotoxic treatment with hydrogen peroxide and γ-radiation were examined by neutral comet assay. MTT assay was used for cell viability analysis and Annexin V-FITC kit specific for kinase-3 was employed to determine apoptosis. Results: Lymphocytes from HNSCC patients were sensitive to genotoxic treatment and displayed impaired DSBs repair. Finally, as a consequence of this finding we have evidenced higher rate of apoptosis induction after γ-radiation treatment of lymphocytes from HNSCC patients than those from healthy controls. Conclusions: DSBs repair and increased apoptosis in cells of patients with head and neck cancer is relevant for efficient therapy of HNSCC. This work was supported by grant N301 099 32/3581 from Polish Ministry of Science and Higher Education. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients Article published earlier |
| spellingShingle | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients Rusin, P. Markiewicz, L. Olszewski, J. Morawiec-Sztandera, A. Kowalski, M. Przybylowska, K. Kaczmarczyk, D. Kusmierczyk, K. Majstereksup, I. Original contributions |
| title | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients |
| title_full | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients |
| title_fullStr | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients |
| title_full_unstemmed | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients |
| title_short | DNA double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients |
| title_sort | dna double strand breaks repair and apoptosis induction in peripheral blood lymphocytes of head and neck cancer patients |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138137 |
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