Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy

Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy

Photodynamic therapy leads to oxidative stress through the generation of free radicals. Oxidative stress causes damage to cellular macromolecules such as nucleic acids, proteins and lipids. Aim: To examine the hematoporphyrin derivative (HpD) — mediated photodynamic effect on cervical adenocarcinoma...

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Published in:Experimental Oncology
Date:2009
Main Authors: Saczko, J., Skrzypek, W., Chwilkowska, A., Choromanska, A., Pola, A., Gamian, A., Kulbacka, J.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/138206
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy / J. Saczko, W. Skrzypek, A. Chwilkovska, A. Choromanska, A. Pola, A. Gamian, J. Kulbaska // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 195-199. — Бібліогр.: 39 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138206
record_format dspace
spelling Saczko, J.
Skrzypek, W.
Chwilkowska, A.
Choromanska, A.
Pola, A.
Gamian, A.
Kulbacka, J.
2018-06-18T10:59:05Z
2018-06-18T10:59:05Z
2009
Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy / J. Saczko, W. Skrzypek, A. Chwilkovska, A. Choromanska, A. Pola, A. Gamian, J. Kulbaska // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 195-199. — Бібліогр.: 39 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138206
Photodynamic therapy leads to oxidative stress through the generation of free radicals. Oxidative stress causes damage to cellular macromolecules such as nucleic acids, proteins and lipids. Aim: To examine the hematoporphyrin derivative (HpD) — mediated photodynamic effect on cervical adenocarcinoma cell line HeLa. Methods: The HpD localization in HeLa cells was analyzed by confocal microscopy with epi-fluorescence system. Lipid peroxidation (LPO) was estimated by measurement of the concentration of malondialdehyde, protein degradation — by modified Ellman’s method, superoxide dysmutase (SOD) — using Ransod Kit. The expression of inducible nitric oxide synthase (iNOS) was detected by immunocytochemical staining. Results: The HpD was distributed all over the cytoplasm with preferential localization in the inner side of the plasma membrane and around the nuclear envelope. The process of photosensitizer distribution was time dependent. PDT-HpD increased the level of malonodialdehyde (MDA), SOD activity and the expression of iNOS in HeLa cells. However, PDT induced the decrease in the level of protein-associated thiol groups. Conclusions: Our study showed the important role of PDT-mediated oxidative stress in HeLa cells. HpD-PDT might be alternative and less invasive approach for treatment of patients with cervical cancer resistant for standard chemotherapy and radiotherapy.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
spellingShingle Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
Saczko, J.
Skrzypek, W.
Chwilkowska, A.
Choromanska, A.
Pola, A.
Gamian, A.
Kulbacka, J.
Original contributions
title_short Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
title_full Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
title_fullStr Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
title_full_unstemmed Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy
title_sort photo-oxidative action in cervix carcinoma cells induced by hpd — mediated photodynamic therapy
author Saczko, J.
Skrzypek, W.
Chwilkowska, A.
Choromanska, A.
Pola, A.
Gamian, A.
Kulbacka, J.
author_facet Saczko, J.
Skrzypek, W.
Chwilkowska, A.
Choromanska, A.
Pola, A.
Gamian, A.
Kulbacka, J.
topic Original contributions
topic_facet Original contributions
publishDate 2009
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Photodynamic therapy leads to oxidative stress through the generation of free radicals. Oxidative stress causes damage to cellular macromolecules such as nucleic acids, proteins and lipids. Aim: To examine the hematoporphyrin derivative (HpD) — mediated photodynamic effect on cervical adenocarcinoma cell line HeLa. Methods: The HpD localization in HeLa cells was analyzed by confocal microscopy with epi-fluorescence system. Lipid peroxidation (LPO) was estimated by measurement of the concentration of malondialdehyde, protein degradation — by modified Ellman’s method, superoxide dysmutase (SOD) — using Ransod Kit. The expression of inducible nitric oxide synthase (iNOS) was detected by immunocytochemical staining. Results: The HpD was distributed all over the cytoplasm with preferential localization in the inner side of the plasma membrane and around the nuclear envelope. The process of photosensitizer distribution was time dependent. PDT-HpD increased the level of malonodialdehyde (MDA), SOD activity and the expression of iNOS in HeLa cells. However, PDT induced the decrease in the level of protein-associated thiol groups. Conclusions: Our study showed the important role of PDT-mediated oxidative stress in HeLa cells. HpD-PDT might be alternative and less invasive approach for treatment of patients with cervical cancer resistant for standard chemotherapy and radiotherapy.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138206
citation_txt Photo-oxidative action in cervix carcinoma cells induced by HpD — mediated photodynamic therapy / J. Saczko, W. Skrzypek, A. Chwilkovska, A. Choromanska, A. Pola, A. Gamian, J. Kulbaska // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 195-199. — Бібліогр.: 39 назв. — англ.
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AT skrzypekw photooxidativeactionincervixcarcinomacellsinducedbyhpdmediatedphotodynamictherapy
AT chwilkowskaa photooxidativeactionincervixcarcinomacellsinducedbyhpdmediatedphotodynamictherapy
AT choromanskaa photooxidativeactionincervixcarcinomacellsinducedbyhpdmediatedphotodynamictherapy
AT polaa photooxidativeactionincervixcarcinomacellsinducedbyhpdmediatedphotodynamictherapy
AT gamiana photooxidativeactionincervixcarcinomacellsinducedbyhpdmediatedphotodynamictherapy
AT kulbackaj photooxidativeactionincervixcarcinomacellsinducedbyhpdmediatedphotodynamictherapy
first_indexed 2025-12-02T08:42:25Z
last_indexed 2025-12-02T08:42:25Z
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