Antiangiogenic properties of a nutrient mixture in a model of hemangioma

Antiangiogenic properties of a nutrient mixture in a model of hemangioma

The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant ant...

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Published in:Experimental Oncology
Date:2009
Main Authors: Roomi, M.W., Kalinovsky, T., Niedzwiecki, A., Rath, M.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2009
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Online Access:https://nasplib.isofts.kiev.ua/handle/123456789/138207
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Journal Title:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Cite this:Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138207
record_format dspace
spelling Roomi, M.W.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
2018-06-18T10:59:38Z
2018-06-18T10:59:38Z
2009
Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138207
The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines. Aim: Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating cell viability, MMP secretion, invasion, morphology and apoptosis. Methods: Athymic nude mice, 5–6 weeks old, were inoculated with 3 x106 EOMA cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B — a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM in vitro. Results: NM inhibited the growth of tumors by 50%. In vitro, NM exhibited dose response cytotoxicity with 10%, 30% and 55% at 10, 100 and 1000 μg/ml. Invasion through Matrigel was inhibited at 50, 100 and 500 μg/ml by 25%, 30% and 100% respectively. NM induced dose-dependent apoptosis of EOMA cells. Conclusions: These results suggest that NM may have therapeutic potential in treating infantile hemangioendotheliomas and, perhaps, other cutaneous vascular tumors.
The research study was funded by Dr. Rath Health Foundation (Plantation, Florida, USA), a non-profit organization.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Antiangiogenic properties of a nutrient mixture in a model of hemangioma
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Antiangiogenic properties of a nutrient mixture in a model of hemangioma
spellingShingle Antiangiogenic properties of a nutrient mixture in a model of hemangioma
Roomi, M.W.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
Original contributions
title_short Antiangiogenic properties of a nutrient mixture in a model of hemangioma
title_full Antiangiogenic properties of a nutrient mixture in a model of hemangioma
title_fullStr Antiangiogenic properties of a nutrient mixture in a model of hemangioma
title_full_unstemmed Antiangiogenic properties of a nutrient mixture in a model of hemangioma
title_sort antiangiogenic properties of a nutrient mixture in a model of hemangioma
author Roomi, M.W.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
author_facet Roomi, M.W.
Kalinovsky, T.
Niedzwiecki, A.
Rath, M.
topic Original contributions
topic_facet Original contributions
publishDate 2009
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines. Aim: Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating cell viability, MMP secretion, invasion, morphology and apoptosis. Methods: Athymic nude mice, 5–6 weeks old, were inoculated with 3 x106 EOMA cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B — a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM in vitro. Results: NM inhibited the growth of tumors by 50%. In vitro, NM exhibited dose response cytotoxicity with 10%, 30% and 55% at 10, 100 and 1000 μg/ml. Invasion through Matrigel was inhibited at 50, 100 and 500 μg/ml by 25%, 30% and 100% respectively. NM induced dose-dependent apoptosis of EOMA cells. Conclusions: These results suggest that NM may have therapeutic potential in treating infantile hemangioendotheliomas and, perhaps, other cutaneous vascular tumors.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138207
citation_txt Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ.
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AT kalinovskyt antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma
AT niedzwieckia antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma
AT rathm antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma
first_indexed 2025-11-30T17:00:56Z
last_indexed 2025-11-30T17:00:56Z
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