Antiangiogenic properties of a nutrient mixture in a model of hemangioma
The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant ant...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2009 |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2009
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862635011316908032 |
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| author | Roomi, M.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. |
| author_facet | Roomi, M.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. |
| citation_txt | Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines. Aim: Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating cell viability, MMP secretion, invasion, morphology and apoptosis. Methods: Athymic nude mice, 5–6 weeks old, were inoculated with 3 x106 EOMA cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B — a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM in vitro. Results: NM inhibited the growth of tumors by 50%. In vitro, NM exhibited dose response cytotoxicity with 10%, 30% and 55% at 10, 100 and 1000 μg/ml. Invasion through Matrigel was inhibited at 50, 100 and 500 μg/ml by 25%, 30% and 100% respectively. NM induced dose-dependent apoptosis of EOMA cells. Conclusions: These results suggest that NM may have therapeutic potential in treating infantile hemangioendotheliomas and, perhaps, other cutaneous vascular tumors.
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| first_indexed | 2025-11-30T17:00:56Z |
| format | Article |
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| id | nasplib_isofts_kiev_ua-123456789-138207 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-11-30T17:00:56Z |
| publishDate | 2009 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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| spelling | Roomi, M.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. 2018-06-18T10:59:38Z 2018-06-18T10:59:38Z 2009 Antiangiogenic properties of a nutrient mixture in a model of hemangioma / M.W. Roomi, T. Kalinovsky, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2009. — Т. 31, № 4. — С. 214-219. — Бібліогр.: 33 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138207 The pathogenesis of hemangiomas is still largely unknown and the current therapy, such as systemic corticosteroid, vincristine, and interferon-alpha, is toxic and remains unsatisfactory. A nutrient mixture (NM) containing lysine, proline, ascorbic acid and green tea extract has shown significant anti-angiogenic and anti-tumor effect against a number of cancer cell lines. Aim: Using a mouse hemangioendothelioma model, we investigated the efficacy of NM. We also tested the effect of NM in vitro, evaluating cell viability, MMP secretion, invasion, morphology and apoptosis. Methods: Athymic nude mice, 5–6 weeks old, were inoculated with 3 x106 EOMA cells subcutaneously and randomly divided into two groups; group A was fed a regular diet and group B — a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighed and processed for histology. We also tested the effect of NM in vitro. Results: NM inhibited the growth of tumors by 50%. In vitro, NM exhibited dose response cytotoxicity with 10%, 30% and 55% at 10, 100 and 1000 μg/ml. Invasion through Matrigel was inhibited at 50, 100 and 500 μg/ml by 25%, 30% and 100% respectively. NM induced dose-dependent apoptosis of EOMA cells. Conclusions: These results suggest that NM may have therapeutic potential in treating infantile hemangioendotheliomas and, perhaps, other cutaneous vascular tumors. The research study was funded by Dr. Rath Health Foundation (Plantation, Florida, USA), a non-profit organization. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Antiangiogenic properties of a nutrient mixture in a model of hemangioma Article published earlier |
| spellingShingle | Antiangiogenic properties of a nutrient mixture in a model of hemangioma Roomi, M.W. Kalinovsky, T. Niedzwiecki, A. Rath, M. Original contributions |
| title | Antiangiogenic properties of a nutrient mixture in a model of hemangioma |
| title_full | Antiangiogenic properties of a nutrient mixture in a model of hemangioma |
| title_fullStr | Antiangiogenic properties of a nutrient mixture in a model of hemangioma |
| title_full_unstemmed | Antiangiogenic properties of a nutrient mixture in a model of hemangioma |
| title_short | Antiangiogenic properties of a nutrient mixture in a model of hemangioma |
| title_sort | antiangiogenic properties of a nutrient mixture in a model of hemangioma |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138207 |
| work_keys_str_mv | AT roomimw antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma AT kalinovskyt antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma AT niedzwieckia antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma AT rathm antiangiogenicpropertiesofanutrientmixtureinamodelofhemangioma |