Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications

Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications

Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by exp...

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Збережено в:
Бібліографічні деталі
Опубліковано в: :Experimental Oncology
Дата:2017
Автори: Vasyliev, R.G., Rodnichenko, A.E., Gubar, O.S., Zlatska, A.V., Gordiienko, I.M., Novikova, S.N., Zubov, D.O.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2017
Теми:
Original contributions
Онлайн доступ:https://nasplib.isofts.kiev.ua/handle/123456789/138539
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R.G. Vasyliev, A.E. Rodnichenko, O.S. Gubar, A.V. Zlatska, I.M. Gordiienko, S.N. Novikova, D.O. Zubov // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 171–180. — Бібліогр.: 29 назв. — англ.

Репозитарії

Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138539
record_format dspace
spelling Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
2018-06-19T09:10:03Z
2018-06-19T09:10:03Z
2017
Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R.G. Vasyliev, A.E. Rodnichenko, O.S. Gubar, A.V. Zlatska, I.M. Gordiienko, S.N. Novikova, D.O. Zubov // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 171–180. — Бібліогр.: 29 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138539
Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
spellingShingle Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
Original contributions
title_short Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_full Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_fullStr Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_full_unstemmed Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
title_sort large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications
author Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
author_facet Vasyliev, R.G.
Rodnichenko, A.E.
Gubar, O.S.
Zlatska, A.V.
Gordiienko, I.M.
Novikova, S.N.
Zubov, D.O.
topic Original contributions
topic_facet Original contributions
publishDate 2017
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
description Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2⁺Sox10⁺Nestin⁺CD73⁺CD90⁺CD105⁺CD140a⁺CD 140b⁺CD146⁺CD166⁺CD271⁺CD349⁺ CD34⁻CD45⁻CD56⁻HLA⁻DR⁻, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138539
citation_txt Large-scale expansion and characterization of human adult neural crest-derived multipotent stem cells from hair follicle for regenerative medicine applications / R.G. Vasyliev, A.E. Rodnichenko, O.S. Gubar, A.V. Zlatska, I.M. Gordiienko, S.N. Novikova, D.O. Zubov // Experimental Oncology. — 2017 — Т. 39, № 3. — С. 171–180. — Бібліогр.: 29 назв. — англ.
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first_indexed 2025-12-07T16:45:33Z
last_indexed 2025-12-07T16:45:33Z
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