Adsorption-rheological properties of blood serum in lung cancer patients
The aim and objectives of the study were to investigate the state of adsorption-rheological properties of blood (ARPB) in patients with different clinical course of lung cancer (LC), the detection of violations of surface-active, viscoelastic and relaxation properties of blood serum, their associati...
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
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| Cite this: | Adsorption-rheological properties of blood serum in lung cancer patients / Y.V. Dumanskiy, O.Y. Stoliarova, O.V. Syniachenko, M.F. Giulmamedova, Y.A. Potapov // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 304–307. — Бібліогр.: 17 назв. — англ. |
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| author | Dumanskiy, Y.V. Stoliarova, O.Y. Syniachenko, O.V. Giulmamedova, M.F. Potapov, Y.A. |
| author_facet | Dumanskiy, Y.V. Stoliarova, O.Y. Syniachenko, O.V. Giulmamedova, M.F. Potapov, Y.A. |
| citation_txt | Adsorption-rheological properties of blood serum in lung cancer patients / Y.V. Dumanskiy, O.Y. Stoliarova, O.V. Syniachenko, M.F. Giulmamedova, Y.A. Potapov // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 304–307. — Бібліогр.: 17 назв. — англ. |
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| description | The aim and objectives of the study were to investigate the state of adsorption-rheological properties of blood (ARPB) in patients with different clinical course of lung cancer (LC), the detection of violations of surface-active, viscoelastic and relaxation properties of blood serum, their association with tumor markers, the evaluation of the prognostic value of initial indexes in the development of complications from radiochemotherapy. Patients and Methods: The study included 115 patients with LC at the age from 24 to 80 years (average age 58 years), among whom there were 78% men and 22% women. The parameters of surface (interfacial) viscosity, elasticity, viscoelasticity module, tension and relaxation of blood serum were studied by the oscillating drop method using a computer tensiometer “PAT2-Sinterface”, and its volumetric viscosity was investigated using a Low-Shear-30 rotational viscometer. ARPB parameters were also studied in a control group composed from 50 healthy donors. Results: Increased levels of volumetrical viscosity, surface tension, surface elasticity and the relaxation time of the blood are typical for patients with LC and depended on the localization of the tumor, its histological variant, differentiation grade, severity of the course of the disease, the number of metastases in the lymph nodes, distant organs and skeleton, involvement of the pleura and ribs, the development of compression pulmonary syndrome, metastasis into the spine, adrenals, brain, and pancreas. The surface-active, viscoelastic and relaxation properties of the blood correlated with the levels of tumor markers (TGFβ1, VEGF, C-reactive protein, α2-macroglobulin). Conclusions: Integral changes of ARPB observed in every fifth patient with LC are involved in the pathogenesis of the disease, have predictive value in relation to the clinical course of disease (volumetric viscosity) and the development of complications from radiochemotherapy (surface viscosity).
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304 Experimental Oncology 39, 304–307, 2017 (December) Experimental Oncology 39, 304–307, 2017 (December) 305
ADSORPTION-RHEOLOGICAL PROPERTIES OF BLOOD SERUM
IN LUNG CANCER PATIENTS
Y.V. Dumanskiy1, *, O.Y. Stoliarova2, O.V. Syniachenko1, M.F. Giulmamedova1, Y.A. Potapov1
1Donetsk National Medical University, Lyman 84400, Ukraine
2National Cancer Institute, Kyiv 03022, Ukraine
The aim and objectives of the study were to investigate the state of adsorption-rheological properties of blood (ARPB) in patients with
different clinical course of lung cancer (LC), the detection of violations of surface-active, viscoelastic and relaxation properties of blood
serum, their association with tumor markers, the evaluation of the prognostic value of initial indexes in the development of complica-
tions from radiochemotherapy. Patients and Methods: The study included 115 patients with LC at the age from 24 to 80 years (aver-
age age 58 years), among whom there were 78% men and 22% women. The parameters of surface (interfacial) viscosity, elasticity,
viscoelasticity module, tension and relaxation of blood serum were studied by the oscillating drop method using a computer tensiometer
“PAT2-Sinterface”, and its volumetric viscosity was investigated using a Low-Shear-30 rotational viscometer. ARPB parameters were
also studied in a control group composed from 50 healthy donors. Results: Increased levels of volumetrical viscosity, surface tension,
surface elasticity and the relaxation time of the blood are typical for patients with LC and depended on the localization of the tumor, its
histological variant, differentiation grade, severity of the course of the disease, the number of metastases in the lymph nodes, distant
organs and skeleton, involvement of the pleura and ribs, the development of compression pulmonary syndrome, metastasis into the
spine, adrenals, brain, and pancreas. The surface-active, viscoelastic and relaxation properties of the blood correlated with the levels
of tumor markers (TGFβ1, VEGF, C-reactive protein, α2-macroglobulin). Conclusions: Integral changes of ARPB observed in every
fifth patient with LC are involved in the pathogenesis of the disease, have predictive value in relation to the clinical course of disease
(volumetric viscosity) and the development of complications from radiochemotherapy (surface viscosity).
Key Words: cancer, lung, blood, serum, adsorption, rheology.
Lung cancer (LC) is the leader in the structure
of cancer morbidity and mortality [1, 2]. An impor-
tant pathogenetic link supporting the properties
of LC is neoangiogenesis (the formation of new ves-
sels) [3]. At the same time, the expression of pro-
tein products that are surfactants (surface active
substances) or possessing insurfactant properties
(surface-inactive) in patients with LC provides the pro-
cesses of neoangiogenesis and the physicochemical
interfacial state of blood serum [4]. There is a close
intercommunication between the biochemical compo-
nents and the functional activity of the cells in tumor
microenvironment, capable to secrete surfactants/
insurfactants influencing biochemical processes, and
thus the adsorption-rheological properties of blood
(ARPB) — surface tension (ST), serum viscosity (SV),
serum elasticity (SE), serum relaxation (SR), and vis-
coelasticity (VE) modulus [5, 6].
The interfacial tensiometry method is used to study
the ST of cancer cell cultures as a criterion of their
aggressiveness [7, 8], as well as to evaluate the ef-
fect of antitumor drugs in vitro [6], in particular, in the
context of the character of the drug load on cancer
cells [9, 10]. It has been shown that the inhibitory ef-
fect on the viability of tumor cells occurs in the cases
when ST of the culture medium decreased [11]. Taking
into consideration the different activity of suspensions
of normal and tumor cells at interphase (“blood”/
“air”), it is considered possible to selecting individual
treatment of patients with LC on the basis of similar
studies in vitro [12]. There is an opinion that ARPB
can be used to monitor the effectiveness of antitumor
radiochemotherapy [13].
The purposes and tasks of the study were to in-
vestigate the state of ARPB in patients with different
variants of LC course, to determine the practical sig-
nificance of violations of surface-active, viscoelastic
and relaxation properties of blood serum, their inter-
relationships with tumor markers, to evaluate the
prognostic role of initial indices in the development
of complications of radiochemotherapy.
MATERIALS AND METHODS
The work was carried out in accordance with the
ethical norms set forth in the Declaration of the Hel-
sinki Medical Assembly, the patients gave informed
consent for the study, which was approved by the
commission on bioethics of the Donetsk National
Medical University.
115 patients with LC aged 24 to 80 years (mean
57.9 ± 1.1 years) were under the observation, among
them there were 78.3% of men and 21.7% of women.
None of the examined patients received previously
surgical treatment of LC. Localization of LC was right-
sided in 59.1% of the cases, left-sided — in 38.3%,
bilateral — in 2.6%; in the upper lobes of the lungs —
in 23.5% of cases, in the lower lobes — in 13.9%,
in the upper-lower-lobe on the left — in 19.1%, the
middle-upper-lobe on the right — in 30.4%, the me-
diastinal — in 13.0%. The central form of LC occurred
in 77.4% of cases, peripheral — in 22.6%.
Submitted: May 10, 2017.
Correspondence: E-mail: oncologdopс@gmail.com
Abbreviations used: ARPB — adsorption-rheological properties
of blood; CT — chemotherapy; ISI — integral severity index of tu-
mor process; LC — lung cancer; RT — radiotherapy; SE — surface
elasticity; SR — surface relaxation; ST — surface tension; SV —
surface viscosity; VE — viscoelasticity; VV — volume viscosity.
Exp Oncol 2017
39, 4, 304–307 Small cell histological variant of LC was established
in 16.5% of cases, non-small cell histological vari-
ant of LC — in 83.5%, including adenocarcinoma —
in 40.0%, squamous cell carcinoma — in 38.3%, large
cell carcinoma — in 5.2%. IIIA stage of the tumor pro-
cess was observed in 12.2% of cases, IIIB — in 18.3%,
IV — in 69.6%. The degree of differentiation of LC was
1.15 ± 0.108 points, the staging — 6.57 ± 0.065 r.u.,
the integral severity index of tumor process (ISI) was
3.97 ± 0.056 r.u., which was calculated by the formula:
ISI = ln [T + N2 + (SM)2],
where ln is the decimal logarithm, T is the inter-
national index of the primary tumor, N is the interna-
tional index of metastasis in regional lymph nodes, and
SM is the sum of metastases in distant organs. The
course of the disease in 12.2% of patients was compli-
cated by exudative pleurisy, in 11.3% — compression
syndrome, 10.4% — tracheal invasion, in 5.2% — inva-
sion in the chest wall and compression of the recurrent
nerve, in 4.4% — respectively obstructive atelectasis
and compression of the upper vena cava, in 3.8% —
tumor invasion in the esophagus, in 2.6% — in the ribs.
Metastatic spreading of LC to lymph nodes was
found in 96.5% of observations, in distant organs —
in 54.8%, in the skeleton — in 51.3%, and the average
number of metastases per one patient was respectively
2.42 ± 0.136 a.u., 1.86 ± 0.137 a.u. and 3.03 ± 0.192 a.u.
For the diagnosis of LC and its metastases, the meth-
ods of radiography, computed tomography, magnetic
resonance imaging and sonography (Multix-Compact-
Siemens, Germany; Somazom-Emotion-6-Siemens,
Germany; Gygoscan-Intera-Philips, Netherlands;
Envisor-Philips, Netherlands), esophagogastroscopy
(fibroscope Olympus-GIF-Q20, Japan) were used. The
parameters of the blood (SV, SE, ST, SR, and VE) were
assessed using a computer tensiometer PAT2-Sinterface
(Germany), the volume viscosity of the blood (VV) was ex-
amined using a rotary viscosimeter Low-Shear-30 (Swit-
zerland). In our studies, a rapid stress deformation
of the expansion of the blood serum surface was used
(at t = 1200 s). The integral degree of change of ARPB
(X) indices was determined by the formula:
2
1 ∑
n
i=1 SD
(М1 – М2)
n ,
where M1 is the index in the patient, M2 is the ave-
rage index in healthy persons of the control group,
SD is the standard deviation of the index in healthy
persons. The modified value was considered to be X
> 2 r.u. As control, the parameters of ARPB were stu-
died in the blood serum of 50 practically healthy people
aged 18 to 62 years (27 men and 23 women). Using
immunoenzyme and biochemical methods (the reader
PR2100-Sanofi Diagnostic Pasteur, France; Olympus-
AU640 Analyzer, Japan), the levels of TGFβ1, VEGF, os-
teopontin, osteocalcin, C-reactive protein, fibronectin
and α2-macroglobulin in the blood serum were studied.
Statistical analysis of the data was carried out
using computer variational, nonparametric, cor-
relative, regressive, one- (ANOVA) and multifactorial
(ANOVA/MANOVA) dispersion analysis (Microsoft Ex-
cel and Statistica-Stat-Soft, USA). Mean values (M),
their standard deviations (SD) and errors (m),
Pearson’s correlation coefficients (r), regression
criteria (R), dispersions of Brown — Forsythe (BF),
Student (t), Wilcoxon — Rao (WR) and the reliability
of statistical indices (p) were estimated. The values
p < 0.05 were considered significant.
RESULTS AND DISCUSSION
The indices X > 2 r.u. were found in 20.9% of the
patients with LC, which are conditionally included in the
main group. If in healthy people the VV parameters
were 1.3 ± 0.03 mPa•s, SV — 15.5 ± 0.23 mN/m, SE —
42.8 ± 0.68 mN/m, ST — 42.8 ± 0.25 mN/m, SR —
114.4 ± 3.03 s, VE — 23.7 ± 1.04 mN/m, the values of VV,
SE, ST and SR were significantly higher in patients with
LC, which (> M + SD healthy), were respectively detected
in 100.0; 5.2; 18.3 and 16.5% of cases (Fig. 1, 2).
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0
VV, mPa · s
Fig. 1. Histograms of the VV index in patients with LC (dark curve)
and healthy people (light curve)
SR, s
40 60 80 100 120 140 160 180 200
Fig. 2. Histograms of the SR index in patients with LC (dark
curve) and healthy people (light curve)
According to the findings of the multifactorial disper-
sion analysis of Wilcoxon — Rao, the localization of LC
(WR = 1.58, p = 0.019), the presence of compression
pulmonary syndrome (WR = 1.64, p = 0.013), the number
of lymph node metastases per one patient (WR = 2.04,
p = 0.001) and to distant organs (WR = 3.55, p < 0.001)
http://exp-oncology.com.ua/article/organization/maksim-gorky-donetsk-national-medical-university-krasnyi-lyman-84400-ukraine
http://exp-oncology.com.ua/article/organization/national-cancer-institute-kyiv-03022-ukraine
306 Experimental Oncology 39, 304–307, 2017 (December) Experimental Oncology 39, 304–307, 2017 (December) 307
influence the integral state of ARPB. According to the
Brown — Forsythe analysis, the parameters SV (BF = 2.69,
p = 0.013), which in men proved to be significantly (by 8%)
large (t = 2.87, p = 0.005) are associated with the sex of pa-
tients with LC. Indices of VV are affected by the small-cell
form of LC (BF = 5.46, p = 0.021), the presence of com-
pression pulmonary syndrome (BF = 4.16, p = 0.044),
adrenal metastases (BF = 6.99, p = 0.009) and the ribs
(BF = 7.37, p = 0.008), the number of metastases in lymph
nodes per a patient (BF = 5.39, p = 0.022), SV is af-
fected by development of exudative pleurisy (BF = 2.69,
p = 0.013), tumor invasion in the ribs (BF = 3.33, p = 0.003)
and meta stasis in the brain (BF = 2.74, p = 0.012),
SE is affected by metastases in subclavian lymph nodes
(BF = 1.79, p = 0.041), the sternum (BF = 1.82, p = 0.036),
the humerus (BF = 2.60, p = 0.002) and the spinal column
(BF = 3.20, p < 0.001), ST is affected by the index of dif-
ferentiation of LC (BF = 2,26, p = 0,047), SR is affected
by metastases in the spine (BF = 4.61, p = 0.038) and pan-
creas (BF = 3.22, p < 0.001), VE is affected by metastases
in the spine (BF = 1.74, p = 0.037) and non-small cell LC
(BF = 2.34, p = 0.003).
Analysis of multiple regression showed a direct
dependence of VV on the integral state of clinical signs
of LC (R = +2.28, p = 0.025), and on the character of tu-
mor metastasis and the number of metastases in lymph
nodes (R = +2.74, p = 0.007). VV directly correlates with
ISI (r = +0.231, p = 0.013) and the number of metastases
in the lymph nodes (r = +0.360, p < 0.001), and SR and
VE values are inversely correlated with the number of me-
tastases in distant organs (r = −0.189, p = 0.043 and
r = −0.238, p = 0.011), which is reflected in Fig. 3 and 4.
There are no correlations between separate indexes
of ARPB and such tumor markers as osteopontin, os-
teocalcin and fibronectin. In turn, with all the parameters
of ARPB without exception, there are direct relations
between the level of VEGF, which, in addition to the
prognostic factor of high aggression of LC [14–16],
is an important component in the development of neo-
angiogenesis in such patients [17]. In addition to the
above, VV positively correlates with TGFβ1 (r = +0.197,
p = 0.035) and C-reactive protein (r = +0.293, p = 0.002),
and ST and SR directly correlates with TGFβ1 (respec-
tively R = +0.209, p = 0.025 and r = +0.191, p = 0.042)
and inversely with α2-macroglobulin (r = −0.190,
p = 0.042 and r = −0.266, p = 0.004).
In deceased patients with X < 2 r.u. the life expec-
tancy was on average six months longer than in the main
group of the examined persons, respectively amount-
ing to 11.3 ± 1.41 months and 17.9 ± 6.96 months, but
the differences were unreliable. The life expectancy
of such patients was influenced by the initial VV pa-
rameters (BF = 1.94, p = 0.018) and SV (BF = 1.71,
p = 0.048), and a direct correlation was established
with SE, ST and VE levels (r = +0.279, p = 0.007,
r = +0.245, p = 0.019, r = +0.360, p < 0.001).
According to the results of ANOVA/MANOVA, the
character of tumor metastasis in the lymph nodes
(WR = 1.92, p = 0.048) and the skeleton (WR = 1.71,
p = 0.049) affects the integral state of ARPB in patients
with LC. As ANOVA shows, the number of metastases
in the lymph nodes per one patient (correspondingly BF =
3.79, p = 0.002 and BF = 3.30, p = 0.005) is related to the
levels of VV and VE. In turn, the process of metastatic
spreading in the bone is determined by the parameters
of viscosity of blood (BF = 2.26, p = 0.043), and in dis-
tant organs — VE of the serum (BF = 2.93, p = 0.016).
Correspondingly, ISI differently depends on VV and VE
(R = +2.24, p = 0.027 and R = −2.42, p = 0.017, respec-
tively), metastatic spreading into the lymph nodes de-
pends only on VV (R = +3.65, p < 0.001), and metastatic
spreading into distant organs depends on VE (R = −4.98,
p < 0.001). Metastases in the osteoarticular apparatus
are closely related to the relaxation properties of the
blood (R = +3.30, p = 0.001). We suppose that the indices
in the blood serum VV > 3.5 mPa•s (> M + SD patients)
reflect the severity of the unfavorable course of LC.
Radical radiation therapy (RT) of the primary tu-
mor (> 60 Gy) was received by 5.2% of the number
of examined patients, radical RT in combination with
chemotherapy (CT) — 21.7%, palliative RT of the
lungs — 10.4% of patients, palliative RT with CT —
36.5%, palliative RT for distant metastases — 10.4%,
palliative RT in combination with CT — 36.5%, pallia-
R = -2.6983+1.6946*x
2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8
VV, mPa · s
0
1
2
3
4
5
6
7
Nu
m
be
r o
f m
et
as
ta
se
s
r = +0.3599, p = 0.00008
Fig. 3. Correlation-regression relationships of the VV indica-
tion with the number of metastases in the lymph nodes of the
patients with LC
0
1
2
3
4
5
6
7
Nu
m
be
r o
f m
et
as
ta
se
s
R = 2.1351-0.0438*x
10 15 20 25 30 35 40
VE, mN/m
r = -0.2380, p = 0.0108
Fig. 4. Correlation-regression relations of the VE indication with
the number of metastases in the distant organs of the patients
with LC
tive RT for distant organs — 10.4%, in combination
with CT — 15.7%. In general, RT was received by all
patients, and CT was received by 85 (73.9%) of them.
At the same time, the power of treatment was evalu-
ated. Thus, the average doses of chemotherapeutic
drugs {< M + SD} were defined as minimal (1 point),
the doses {M + SD M + 2SD} — as moderate
(2 points), {M + 2SD M + 3SD} — as high (3 points)
and {> M + 3SD} — as very high (4 points). The power
of RT (W) and CT (Y) was calculated by the formula:
W(Y) = (a + b + c + d) : n,
where a, b, c, d — respectively the number of zones
of irradiation or preparations in 1, 2, 3 and 4 points,
n — the total number of irradiation zones and the
total number of used drugs. The average parameters
W were 1.39 ± 0.076 r.u., and Y — 0.37 ± 0.032 r.u.
As evidenced by the multifactorial analysis of Wil-
coxon — Rao, the character of complications of radio-
chemotherapy for LC is closely related to the overall
condition of ARPB (WR = 4.57, p < 0.001). The number
of complications from the treatment per a patient is sig-
nificantly influenced by the initial indices in blood VV
(BF = 20.01, p < 0.001), SV (BF = 4.86, p = 0.001), SE
(BF = 4. 20, p = 0.003), ST (BF = 6.31, p < 0.001), and
SR (BF = 3.07, p = 0.020), it is demonstrated by ANO-
VA. At the same time, the values of VV have a dispersion
effect on the development of radiation pneumofibrosis
(BF = 5.06, p = 0.026), the formation of myelodepres-
sion (BF = 9.68, p = 0.002) and the appearance of acute
disturbance of cerebral circulation of different degree
of severity (BF = 27,77, p < 0.001), the parameters
SE and SR — on the appearance of acute radiation
pneumonitis (respectively, BF = 5.37, p = 0.022 and
BF = 4.67, p = 0.033), ST and VE — on acute tubuloin-
terstitial nephritis (BF = 8.62, p = 0.004 and BF = 4.42,
p = 0.038) and acute radiation esophagitis (BF = 8.12,
p = 0.006 and BF = 8.21, p = 0.005).
Analysis of multiple regression shows a direct
dependence of complications of RT and meta-
static spreading of tumor processes on the SV level
(R = +3.98, p < 0.001) and feedback with VE (R = −3.33,
p = 0.002). Taking into account the statistical process-
ing of the obtained data of the study, a conclusion has
been made which has a practical focus: the indices of SV
> 20 mN/m (> M + SD of patients) are prognostic-neg-
ative for possible complications of further radiotherapy
of patients with LC.
CONCLUSIONS
RT in every fifth patient is accompanied by integral
changes in ARPB, which are manifested by an increase
of the levels of VV, SE, ST and SR, depending on the sex
of patients (VE), tumor localization, its histological form,
degree of differentiation and severity of the course (VV,
ST, VE), the number of metastases in lymph nodes,
distant organs and skeleton (VV, VE), involvement of the
pleura and ribs in the process (SV), the development
of compression lung syndrome (VV, SR), metastatic
spreading in the spine (SE, SR, VE), adrenal glands,
the brain (SV), and the pancreas (SR). Surface-active,
viscoelastic and relaxation properties of blood correlate
with the levels of tumor markers such as VEGF, TGFβ1,
C-reactive protein, α2-macroglobulin, and indices
VV and SV possess prognostic significance with respect
to LC course and development of complications of ra-
diochemotherapy respectively.
REFERENCES
1. Ceniceros L, Aristu J, Castanon E, et al. Stereotactic
body radiotherapy (SBRT) for the treatment of inoperable
stage I non-small cell lung cancer patients. Clin Transl Oncol
2015; 55: 213–9.
2. Grаdalska-Lampart M, Karczmarek-Borowska B,
Radziszewska AU. Lung cancer in Podkarpackie region in the
years 2002–2011. Pneumonol Alergol Pol 2015; 83: 109–19.
3. Yamada K, Maishi N, Akiyama K, et al. CXCL12-
CXCR7 axis is important for tumor endothelial cell angiogenic
property. Int J Cancer 2015; 23: 172–8.
4. Zhou L, Pan Y, Xing Y, et al. Effects of Feijining Decoc-
tion on vascular endothelial growth factor protein expression
and changes of T cell subsets in Lewis lung carcinoma-bearing
mice. Biomed Rep 2015; 3: 403–7.
5. Giannopoulou E, Siatis KE, Metsiou D, et al. The in-
hibition of aromatase alters the mechanical and rheological
properties of non-small-cell lung cancer cell lines affecting
cell migration. Biochim Biophys Acta 2015; 1853: 328–37.
6. Rojewska M, Prochaska K, Olejnik A, Rychlik J. Adsorp-
tion properties of biologically active derivatives of quaternary
ammonium surfactants and their mixtures at aqueous/air interface
II. Dynamics of adsorption, micelles dissociation and cytotoxic-
ity of QDLS. Colloids Surf B Biointerfaces 2014; 119: 154–61.
7. Butler CM, Foty RA. Measurement of aggregate cohe-
sion by tissue surface tensiometry. J Vis Exp 2011; 50: 165–9.
8. Winters BS, Shepard SR, Foty RA. Biophysical measure-
ment of brain tumor cohesion. Int J Cancer 2015; 114: 371–9.
9. Laskar P, Samanta S, Ghosh SK, Dey J. In vitro evaluation
of pH-sensitive cholesterol-containing stable polymeric micelles for
delivery of camptothecin. J Colloid Interface Sci 2014; 430: 305–14.
10. Munyendo WL, Zhang Z, Abbad S, et al. Micelles
of TPGS modified apigenin phospholipid complex for oral
administration: preparation, in vitro and in vivo evaluation.
J Biomed Nanotechnol 2013; 9: 2034–47.
11. Jiang L, Shen C, Long X, et al. Rhamnolipids elicit the
same cytotoxic sensitivity between cancer cell and normal cell
by reducing surface tension of culture medium. Appl Microbiol
Biotechnol 2014; 98: 10187–96.
12. Preetha A, Banerjee R, Huilgol N. Dynamic surface
tensiomeSRy of tissues using Langmuir films. Colloids Surf
B Biointerfaces 2015; 40: 35–43.
13. Bloy N, Pol J, Manic G, et al. Radioimmunotherapy
for oncological indications. Oncoimmunology 2014; 3: 954929.
14. Domigan CK, Warren CM, Antanesian V, et al. Auto-
crine VEGF maintains endothelial survival through regulation
of metabolism and autophagy. J Cell Sci 2015; 128: 2236–48.
15. Lin Q, Xue L, Tian T, et al. Prognostic value of serum
IL-17 and VEGF levels in small cell lung cancer. Int J Biol
Markers 2015; 30: 165–75.
16. Ostheimer C, Bache M, Güttler A, et al. Prognostic infor-
mation of serial plasma osteopontin measurement in radiotherapy
of non-small-cell lung cancer. BMC Cancer 2014; 21: 858–65.
17. Akin Kabalak P, Çiledag A, Demir N, et al. Prognostic sig-
nificance of serum vascular endothelial growth factor and angiopoi-
etin-2 in patients with lung cancer. Tuberk Toraks 2015; 63: 71–7.
Copyright © Experimental Oncology, 2017
http://www.ncbi.nlm.nih.gov/pubmed/26243396
http://www.ncbi.nlm.nih.gov/pubmed/26243396
http://www.ncbi.nlm.nih.gov/pubmed/26243396
http://www.ncbi.nlm.nih.gov/pubmed/25754052
http://www.ncbi.nlm.nih.gov/pubmed/25754052
http://www.ncbi.nlm.nih.gov/pubmed/26100110
http://www.ncbi.nlm.nih.gov/pubmed/26100110
http://www.ncbi.nlm.nih.gov/pubmed/26100110
http://www.ncbi.nlm.nih.gov/pubmed/26137245
http://www.ncbi.nlm.nih.gov/pubmed/26137245
http://www.ncbi.nlm.nih.gov/pubmed/26137245
http://www.ncbi.nlm.nih.gov/pubmed/26137245
http://www.ncbi.nlm.nih.gov/pubmed/25450981
http://www.ncbi.nlm.nih.gov/pubmed/25450981
http://www.ncbi.nlm.nih.gov/pubmed/25450981
http://www.ncbi.nlm.nih.gov/pubmed/25450981
http://www.ncbi.nlm.nih.gov/pubmed/24768517
http://www.ncbi.nlm.nih.gov/pubmed/24768517
http://www.ncbi.nlm.nih.gov/pubmed/24768517
http://www.ncbi.nlm.nih.gov/pubmed/24768517
http://www.ncbi.nlm.nih.gov/pubmed/24768517
http://www.ncbi.nlm.nih.gov/pubmed/21505411
http://www.ncbi.nlm.nih.gov/pubmed/21505411
http://www.ncbi.nlm.nih.gov/pubmed/15551307
http://www.ncbi.nlm.nih.gov/pubmed/15551307
http://www.ncbi.nlm.nih.gov/pubmed/24974243
http://www.ncbi.nlm.nih.gov/pubmed/24974243
http://www.ncbi.nlm.nih.gov/pubmed/24974243
http://www.ncbi.nlm.nih.gov/pubmed/24266259
http://www.ncbi.nlm.nih.gov/pubmed/24266259
http://www.ncbi.nlm.nih.gov/pubmed/24266259
http://www.ncbi.nlm.nih.gov/pubmed/25231070
http://www.ncbi.nlm.nih.gov/pubmed/25231070
http://www.ncbi.nlm.nih.gov/pubmed/25231070
http://www.ncbi.nlm.nih.gov/pubmed/?term=Preetha A%5BAuthor%5D&cauthor=true&cauthor_uid=15620838
http://www.ncbi.nlm.nih.gov/pubmed/?term=Banerjee R%5BAuthor%5D&cauthor=true&cauthor_uid=15620838
http://www.ncbi.nlm.nih.gov/pubmed/?term=Huilgol N%5BAuthor%5D&cauthor=true&cauthor_uid=15620838
http://www.ncbi.nlm.nih.gov/pubmed/?term=cancer+esophageal+tensiometry##
http://www.ncbi.nlm.nih.gov/pubmed/?term=cancer+esophageal+tensiometry##
http://www.ncbi.nlm.nih.gov/pubmed/25941606
http://www.ncbi.nlm.nih.gov/pubmed/25941606
http://www.ncbi.nlm.nih.gov/pubmed/25956888
http://www.ncbi.nlm.nih.gov/pubmed/25956888
http://www.ncbi.nlm.nih.gov/pubmed/25956888
http://www.ncbi.nlm.nih.gov/pubmed/26165685
http://www.ncbi.nlm.nih.gov/pubmed/26165685
http://www.ncbi.nlm.nih.gov/pubmed/25416631
http://www.ncbi.nlm.nih.gov/pubmed/25416631
http://www.ncbi.nlm.nih.gov/pubmed/25416631
http://www.ncbi.nlm.nih.gov/pubmed/26167963
http://www.ncbi.nlm.nih.gov/pubmed/26167963
http://www.ncbi.nlm.nih.gov/pubmed/26167963
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| id | nasplib_isofts_kiev_ua-123456789-138545 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T16:56:01Z |
| publishDate | 2017 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Dumanskiy, Y.V. Stoliarova, O.Y. Syniachenko, O.V. Giulmamedova, M.F. Potapov, Y.A. 2018-06-19T09:14:10Z 2018-06-19T09:14:10Z 2017 Adsorption-rheological properties of blood serum in lung cancer patients / Y.V. Dumanskiy, O.Y. Stoliarova, O.V. Syniachenko, M.F. Giulmamedova, Y.A. Potapov // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 304–307. — Бібліогр.: 17 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138545 The aim and objectives of the study were to investigate the state of adsorption-rheological properties of blood (ARPB) in patients with different clinical course of lung cancer (LC), the detection of violations of surface-active, viscoelastic and relaxation properties of blood serum, their association with tumor markers, the evaluation of the prognostic value of initial indexes in the development of complications from radiochemotherapy. Patients and Methods: The study included 115 patients with LC at the age from 24 to 80 years (average age 58 years), among whom there were 78% men and 22% women. The parameters of surface (interfacial) viscosity, elasticity, viscoelasticity module, tension and relaxation of blood serum were studied by the oscillating drop method using a computer tensiometer “PAT2-Sinterface”, and its volumetric viscosity was investigated using a Low-Shear-30 rotational viscometer. ARPB parameters were also studied in a control group composed from 50 healthy donors. Results: Increased levels of volumetrical viscosity, surface tension, surface elasticity and the relaxation time of the blood are typical for patients with LC and depended on the localization of the tumor, its histological variant, differentiation grade, severity of the course of the disease, the number of metastases in the lymph nodes, distant organs and skeleton, involvement of the pleura and ribs, the development of compression pulmonary syndrome, metastasis into the spine, adrenals, brain, and pancreas. The surface-active, viscoelastic and relaxation properties of the blood correlated with the levels of tumor markers (TGFβ1, VEGF, C-reactive protein, α2-macroglobulin). Conclusions: Integral changes of ARPB observed in every fifth patient with LC are involved in the pathogenesis of the disease, have predictive value in relation to the clinical course of disease (volumetric viscosity) and the development of complications from radiochemotherapy (surface viscosity). en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Adsorption-rheological properties of blood serum in lung cancer patients Article published earlier |
| spellingShingle | Adsorption-rheological properties of blood serum in lung cancer patients Dumanskiy, Y.V. Stoliarova, O.Y. Syniachenko, O.V. Giulmamedova, M.F. Potapov, Y.A. Original contributions |
| title | Adsorption-rheological properties of blood serum in lung cancer patients |
| title_full | Adsorption-rheological properties of blood serum in lung cancer patients |
| title_fullStr | Adsorption-rheological properties of blood serum in lung cancer patients |
| title_full_unstemmed | Adsorption-rheological properties of blood serum in lung cancer patients |
| title_short | Adsorption-rheological properties of blood serum in lung cancer patients |
| title_sort | adsorption-rheological properties of blood serum in lung cancer patients |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138545 |
| work_keys_str_mv | AT dumanskiyyv adsorptionrheologicalpropertiesofbloodseruminlungcancerpatients AT stoliarovaoy adsorptionrheologicalpropertiesofbloodseruminlungcancerpatients AT syniachenkoov adsorptionrheologicalpropertiesofbloodseruminlungcancerpatients AT giulmamedovamf adsorptionrheologicalpropertiesofbloodseruminlungcancerpatients AT potapovya adsorptionrheologicalpropertiesofbloodseruminlungcancerpatients |