Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk
Aim: Evaluation of chromosomal radiosensitivity of healthy individuals and determination those with the increased susceptibility to radiogenic cancer. Methods: Cytogenetic examination of radiation induced injuries in lymphocytes of healthy individuals (n = 103) was carried out on the basis of G2-ass...
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| Опубліковано в: : | Experimental Oncology |
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| Дата: | 2007 |
| Автори: | , |
| Формат: | Стаття |
| Мова: | Англійська |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2007
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk / E.A. Dyomina, N.M. Ryabchenko // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 217–220. — Бібліогр.: 19 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1860188973167542272 |
|---|---|
| author | Dyomina, E.A. Ryabchenko, N.M. |
| author_facet | Dyomina, E.A. Ryabchenko, N.M. |
| citation_txt | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk / E.A. Dyomina, N.M. Ryabchenko // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 217–220. — Бібліогр.: 19 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | Aim: Evaluation of chromosomal radiosensitivity of healthy individuals and determination those with the increased susceptibility to radiogenic cancer. Methods: Cytogenetic examination of radiation induced injuries in lymphocytes of healthy individuals (n = 103) was carried out on the basis of G2-assay. Test system of peripheral blood lymphocytes with metaphase analysis was used. Results: On the basis of the obtained “stage-effect” and “dose-effect” calibrating curves the scheme of cytogenetic examinations of healthy individuals was developed. Analysis of cytogenetic parameters induced by G2 irradiation at 1.5 Gy dose revealed their high interindividual variability. The highest differences were registered for chromatid type aberrations (CV = 42.1%) with the chromatid break predominance in the spectrum (CV = 37.5%). Statistical analysis of the distributions of the obtained individual cytogenetic parameters indicated 12% individuals with increased chromosomal radiosensitivity. Conclusions: Cytogenetic evaluation of individual chromosomal radiosensitivity based on G2-assay has its perspectives in the formation of groups with increased risk of radiogenic cancer developing and its primary prophylactics among healthy population.
Цель: оценка радиочувствительности здоровых лиц на уровне хромосом лимфоцитов и определение индивидуумов с
повышенным риском радиоиндуцированных новообразований. Методы: цитогенетическое исследование радиационно
индуцированных поврежедений в лимфоцитах здоровых доноров (n = 103) проведено на основе G = 103) проведено на основе G2
-теста. Использована
тест-система лимфоцитов периферической крови с последующим метафазным анализом. Результаты: на основе построения
калибровочных кривых “стадия-эффект”, “доза-эффект” разработана схема цитогенетического обследования условно
здоровых лиц. Анализ величин цитогенетических показателей, полученных при облучении в G2
-периоде клеточного цикла
в дозе 1,5 Гр, выявил их значительную вариабельность. Наибольшая вариабельность наблюдалась для аберраций хроматидного
типа (CV = 42,1%), в спектре которых преобладали одиночные хроматидные разрывы (CV = 37,5%). Статистический
анализ распределений индивидуальных значений полученных показателей позволил выявить 12% лиц с повышенной
радиационной чувствительностью хромосом. Выводы: цитогенетическая оценка индивидуальной радиочувствительности
на хромосомном уровне, основанная на G2
-тесте, имеет перспективы применения при формировании групп повышенного
риска радиоиндуцированных злокачественных новообразований и их первичной профилактики среди населения.
|
| first_indexed | 2025-12-07T18:05:35Z |
| format | Article |
| fulltext |
Experimental Oncology ���� ��������� ���� ��eptem�er�� ������� ��������� ���� ��eptem�er�� ����eptem�er�� ����� ��� ���
The pro�lem of new approaches to the forma-
tion of increased cancer risk groups have �eing an
actual practical and fundamental for a long time and
is closely connected with the mechanisms of cancer
development and its primary prophylactics. Assess-
ment of cytogenetic effects in human peripheral �lood
lymphocytes �PBL�� induced �y test irradiation at one
of the most sensitive cell cycle stage �G�-assay�� is one
of the methods used in the investigations of human
individual radiosensitivity �IR��. Cytogenetic methods
�ased on chromosome a�errations analysis make it
possi�le quantitative estimation of radiation effects
on human organism taking into account its individual
peculiarities and thus to estimate its IR. The main �ases
for application of cytogenetic methods in radio�iology
are high radiosensitivity of human PBL and formation
of specific radiation-induced chromosomal a�erra-
tions [�]. They are considered to �e the proven markers
of cancer development in the calculations of cancer risk
after exposure to ionizing radiation [�]. These data are
extrapolated from the known epidemiological genetic
investigations carried out �y European authors�� who
revealed relia�le correlation �etween cancer incidents
and frequency of chromosomal a�errations in human
somatic cells [3].
The radiosensitivity of cancer patients�� children
evacuated from Cherno�yl area�� children after irradia-
tion of their PBL cultures at adapting doses was de-
termined On the �asis of cytogenetic markers [4�6].
However assessments of IR in the group of healthy
individuals on the �asis of test irradiation of PBL in G�
stage of cell cycle period with the su�sequent analy-
sis of chromosomal a�errations are insufficient and
need further development. Their actuality is o�vious
as they allow o�jective and comprehensive prediction
of potential danger of radiation effects from the point
of view of human pathologies development �ased on
genome insta�ility �first of all cancer and multifactorial
diseases��.
This work presents the data o�tained during the
cytogenetic examinations of healthy individuals with
the purpose to evaluate their chromosomal IR and us-
ing this criterion to determine those with the increased
suscepti�ility to radiogenic cancer.
Materials and Methods
Analysis of aberrations level and spectrum in
chromosomes of human PBL�� which are acknowledged
to �e one of the most sensitive to radiation and are
recommended WHO and UN�CEAR for �iological
indication of the radiation injury of human organism
[8���]�� gives an o�jective information a�out genome
integrity in human somatic cells.
Lymphocytes cultures were esta�lished from
�lood samples of ��3 practically health individuals��
who were informed a�out and agreed with the study.
Cells were cultured according to the standard proce-
dures with modifications [��]. Cells were incu�ated
in RPMI �64� medium�� containing �.� μg/ml PHA
�M form�� Gi�co-Invitrogen�� for 5� h �last 4 h with colce-
mid��. This procedure made it possi�le to analyze cells
in the first post-radiation mitosis. Routine preparations
were made and stained with �% Giemsa solution. The
analysis of painted chromosome preparations was car-
ried out according to the conventional requirements
to metaphase spreads [��]. The study was approved
�y Ethic Committee of IEPOR.
γ-Irradiation �6�Со�� of PBL cultures was carried
out at �.��3.� Gy dose range and �.� Gy/min dose
rate during different cell cycle stages �G� — at � h��
G� — �4 h�� � — 4� h і G� — 46 h of cell incu�ation��.
For G� treatment cultures of PBL were irradiated
with dose of �.5 Gy.
Statistical analysis. Cytogenetic parameters
o�tained were analyzed �y the means of standard
increased individual chroMosoMal radiosensitivity
of huMan lyMphocytes as a paraMeter of cancer risk
E.A. Dyomina, N.M. Ryabchenko*
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine
Aim: Evaluation of chromosomal radiosensitivity of healthy individuals and determination those with the increased susceptibility
to radiogenic cancer. Methods: Cytogenetic examination of radiation induced injuries in lymphocytes of healthy individuals (n =
103) was carried out on the basis of G2-assay. Test system of peripheral blood lymphocytes with metaphase analysis was used. Re-
sults: On the basis of the obtained “stage-effect” and “dose-effect” calibrating curves the scheme of cytogenetic examinations of
healthy individuals was developed. Analysis of cytogenetic parameters induced by G2 irradiation at 1.5 Gy dose revealed their high
interindividual variability. The highest differences were registered for chromatid type aberrations (CV = 42.1%) with the chromatid
break predominance in the spectrum (CV = 37.5%). Statistical analysis of the distributions of the obtained individual cytogenetic
parameters indicated 12% individuals with increased chromosomal radiosensitivity. Conclusions: Cytogenetic evaluation of individual
chromosomal radiosensitivity based on G2-assay has its perspectives in the formation of groups with increased risk of radiogenic
cancer developing and its primary prophylactics among healthy population.
Key Words: individual radiosensitivity, G2-assay, radiogenic cancer.
Received: July 3, 2007.
*Correspondence: E-mail: drozd@onconet.kiev.ua
Abbrevations used: PBL – peripheral blood lymphocytes; IR – in-
dividual radiosensitivity.
Exp Oncol ����
���� 3�� �������
��8 Experimental Oncology ���� ��������� ���� ��eptem�er��
descriptive and variation statistics and included calcu-
lation of mean group values (М)�� standard error �SE����
standard deviation (SD)�� sample dispersion �s2����
coefficient of variation �CV�� etc. and representing
experimental data distri�utions as histograms. After
analysis of their forms and fitting of o�tained func-
tions to normal ones �5% confidential intervals were
determined as M ±�� �6SD. Radiosensitive cut-off point
was also calculated as the ��th percentile of o�tained
G� scores. F-test was applied to indicate significance
of the differences �etween donors. A significance level
of p < �.�5 was used throughout.
results and discussion
Estimation of radiation-induced cytogenetic effects
usually requires the data on spontaneous a�errations
level. It is known to �e not exactly fixed value since it can
�e influenced �y patient age�� modifications in culture
incu�ation�� effects of different mutagenic agents etc.
Therefore the necessity to o�tain own data on a�er-
ration spontaneous level is o�vious. Total frequency
of chromosome a�errations in the examined group �n
= ��3�� was in the limits ���%�� with mean �.4� ± �.�4
a�errations per ��� metaphases. Chromatid deletions
and isodeletions were the most frequently a�erration
types �8�%��. Chromatid-type/chromosome-type ra-
tio was 4.8 : �. Individual differences were formed �y
chromatid a�errations reference for spontaneous mu-
tagenesis. Thus spontaneous levels o�tained differed
among donors and exceed the mean population value
indicated in literature [�3].
Our previous studies have shown that cytogenetic
reaction of human chromosomes to radiation exposure
changes during the cell cycle not only in dependence
on its stage �ut also within the limits of each of them
[�4�� �5]. Thus �efore the determination of IR of healthy
donors the detailed study of PBL chromosomal ra-
diosensitivity after irradiation in different cell cycle
stages was carried out resulting in the o�taining of
own cali�ration “stage-effect” curves. Main cytoge-
netic parameters: % of damaged cells�� total num�er
of a�errations�� levels of chromatid and chromosome
types a�errations�� chromatid and isochromatid �reaks��
chromatid exchanges were examined taking into ac-
count irradiation and culture conditions.
This study revealed two picks in chromosomal
radiosensitivity: at the late G�- and G� -stages while
�-stage was radioresistant. During cell cycle progres-
sion regular change of chromosome-type a�errations
to chromatid one was o�served. At the first half of
cell cycle exchange a�errations prevailed�� while frag-
ments — at the second. Exchanges/fragments ratio
in the first half of cycle equaled � then it gradually
reduced due to the decrease of exchanges. At 3� h of
cell cycle it was less than �. Thus under the irradiation
in G�-�� G�- stages chromosome type a�errations pre-
vail in the spectrum of radiation-induced damages; in
G� — chromatid type and in � — �oth types. The high-
est level of fragments�� namely chromatid �reaks were
o�served after G�-stage irradiation. �u�sequently
we registered next types of chromatid �reaks�� which
o�jectively are recognized according the criterions
�Fig. ���: fragments displaced along chromatid length;
fragments displaced along chromatid axis; fragments
inverted relatively to the axis. Chromatid interstitial de-
letions were also registered as chromatid fragments.
fig. 1. Registered types of chromatid fragments
On the �asis of the o�tained “stage-effect” and
“dose-effect” cali�rating curves the scheme of cy-
togenetic examinations of healthy individuals was
developed �Fig. ���. It assumes connection of G�-assay
principles and the statements of the classical radia-
tion cytogenetics. According to them such factors as
dose value�� cell cycle stage�� post irradiation conditions
effect the estimation of quantitative and qualitative
variations in cell radiation response [�6].
fig. 2. The scheme of cytogenetic experiments on the assessment
of individual radiosensitivity of healthy individuals �PHA — phyto-
haemagglutinin�� R-γ-radiation�� C — colcemid�� F — fixation��
Detailed discussion of the data o�tained with the help
of cytogenetic examinations of healthy donors with the
aim of the assessment of their IR is presented �ellow.
� Dose of test irradiation. Linear dose dependence
of fragments num�er after G� irradiation in wide dose
range was o�tained. We also determined the dose —
���5 Gy which allows to o�tain the values of mitotic index
sufficient for metaphase num�er �mean scored num-
�er — ��� metaphases���� the o�jective estimation of
radiation-induced effects and registration of individual
variations in karyotype sensitivity to test irradiation.
� Examination of test irradiation time in the limits
of radiosensitive G� stage made it possi�le to reveal
highest variations in IR values. Ta�le � presents cyto-
genetic data o�tained after γ-irradiation of PBL culture
at �.5 Gy in the dependence from the time of cell incu-
Experimental Oncology ���� ��������� ���� ��eptem�er�� ������� ��������� ���� ��eptem�er�� ����eptem�er�� ����� ��� ���
�ation during G� stage �4�. 44 and 46 h��. The highest
levels of a�errant lymphocytes �3�.� ± �.����� total num-
�er of a�errations �3�.� ± �.����� chromatid a�errations
�38.� ± �.6�� and chromatid �reaks �3�.� ± �.6�� were
registered after irradiation at 46 h of cell incu�ation��
at the end of G� stage. This term is proposed for test
irradiation as allows o�serving the highest differences
in chromosomal radiosensitivity.
Table 1. Frequencies of chromosomal aberrations in human lymphocytes
exposed to 1.5 Gy γ-rays at G2-stage of cell cycle
Irradiation
time (h)
Abnormal
cells, %
Chromosomal aber-
rations/100 cells
Chromatid aber-
rations/100 cells
Breaks/
100 cells
42 18.5 ± 1.9 22.0 ± 2.1 21.2 ± 2.3 20.0 ± 1.2
44 26.9 ± 2.3 33.0 ± 3.1 29.0 ± 2.5 24.0 ± 2.3
46 31.0 ± 0.9 40.6 ± 1.4 38.0 ± 1.6 32.0 ± 1.6
Radiation-induced cell cycle delay. Fixation of cells
irradiated in G� stage in two terms — 5� h and 58 h after
the �eginning of cultivation was carried out �Fig. 3��. It
was revealed that cytogenetic data of intercellular a�er-
rations distri�ution o�tained after G� test irradiation and
5� h cultivation fitted Poisson distri�ution. This fact testi-
fies that the effect of radiation-induced cell cycle delay
plays insignificant role and cell population is relatively
homogeneous. After fixation at 58 h deviations from the
theoretical distri�ution was o�served�� which indicated
the heterogeneity of cell population �mixture of first and
second post radiation mitosis�� and made these data less
informative [�6�� ��].
� O�servation of standard conditions of cell cul-
tivating�� irradiation and fixation is o�ligatory require-
ment for the analysis and comparison of the o�tained
cytogenetic parameters and o�jective IR estimation
on their �asis. These optimal la�oratory conditions are
indicated in the scheme �see Fig. ��� �ottom line��.
fig. 3. Kinetics of chromosomal a�errations frequencies in
human lymphocytes exposed to γ-rays during cell cycle. Lines
A — fixation on 58 h of cultivation�� rest — on 5� h
The developed scheme of the assessment of IR of
healthy individuals on the �asis of G�-assay was ap-
proved during cytogenetic examination of healthy Kyiv
ha�itants �n = ��3��. Analysis of cytogenetic parameters
induced �y G� irradiation at �.5 Gy dose revealed their
high interindividual varia�ility �Ta�le ���. The highest
differences were registered for chromatid type a�erra-
tions �CV = 4�.�%�� with the chromatid �reak predomi-
nance in the spectrum �84% from the total a�errations
num�er�� up to �5% for individual donors�� CV = 3�.5%��.
This made it possi�le to consider chromatid �reaks
to �e the specific marker of chromosomal radiosen-
sitivity after G� irradiation.
Table 2. G2 radiosensitivity of human lymphocytes derived from healthy
individuals (n = 103) and exposed to 1.5 Gy γ-rays
Cytogenetic parameter Mean number M ± SE SD CV, %
Abnormal cells, % 37.9 ± 1.08 9 29.0
Total aberrations /100 metaphases 40.6 ± 1.8 14 34.5
Chromatid breaks /100 metaphases 32.0 ± 1.6 12 37.5
To indicate the cut-off points of individual variations
of the o�tained IR cytogenetic parameters we used
two approaches: calculation of ��% percentile values
of their distri�utions and analysis of its character. In
our case the value ��% cut-off point was 64 a�erra-
tions/��� metaphases that indicated ��% individuals
with increased chromosomal radiosensitivity.
Analysis of the o�tained cytogenetic parameters’
distri�utions in the referent group on the �asis of varia-
tion statistics showed that they did not fitted normal
distri�ution ��hapiro-Wilki test�� w = �.86; p = �.�5 for
chromatid �reaks��. Two picks in a�erration frequencies
were o�served �Fig. 4��. Fitting the o�tained distri�u-
tion to the sum of two normal ones �R� = �.���� made
it possi�le to indicate mean values of cytogenetic
parameters for two su�groups: individuals with normal
and high chromosomal sensitivity to radiation �Ta�le 3��.
Calculation of �5% confidential interval of their varia-
tion as M ± 2 SD makes it possi�le to know high cut-off
value in group of donors with normal IR: 63.� a�erra-
tions/��� metaphases�� which is practically coincides
with ��-percentile values �64 a�errations/��� meta-
phases�� ��% radiosensitive individuals��. O�served �i-
modality in the distri�ution of IR cytogenetic parameters
induced �y the test irradiation in G� stage of cell cycle
testifies for the heterogeneity of the referent group and
possi�le existence of two populations among healthy
individuals � with normal and enhanced chromosomal
radiosensitivity�� which is determined genetically. It is
suggested that such predisposition to the elevated
G� radiosensitivity is a consequence of the inherited
defects in the efficiency of DNA repair system which
predispose to cancer [5�� �8�� ��].
fig. 4. G� chromosomal radiosensitivity of healthy donors �n =
��3�� at �.5 Gy γ-irradiation of lymphocytes
Table 3. Summary of cytogenetic parameters induced by 1.5 Gy test irra-
daition of lymphocytes of healthy donors from two groups – normal (I) and
elevated (II) G2 chromosomal radiosensitivity
Cytogenetic parameter/100
metaphases
Group
of donors
Мean number
M ± SE SD CV, %
Total number of chromosomal
aberrations
І 37.7 ± 1.8 12.7 33.6
ІІ 74 ± 2.2 8.9 12.0
Chromatid aberrations І 33.7 ± 1.1 12 36.3
ІІ 67 ± 2.4 7 10.4
Chromatid breaks І 27 ± 1.4 10 37
ІІ 61 ± 1.8 8 13
��� Experimental Oncology ���� ��������� ���� ��eptem�er��
Taking into account o�tained results�� cytoge-
netic examination of healthy individuals on the �ase
of G�-assay has its perspectives in the formation of
groups with increased risk of cancer developing and
its primary prophylactics among healthy population.
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ПОВЫШЕННАЯ ИНДИВИДУАЛЬНАЯ
РАДИОЧУВСТВИТЕЛЬНОСТЬ ХРОМОСОМ ЛИМФОЦИТОВ
ЧЕЛОВЕКА КАК ПОКАЗАТЕЛЬ РИСКА ЗЛОКАЧЕСТВЕННЫХ
НОВООБРАЗОВАНИЙ
Цель: оценка радиочувствительности здоровых лиц на уровне хромосом лимфоцитов и определение индивидуумов с
повышенным риском радиоиндуцированных новообразований. Методы: цитогенетическое исследование радиационно
индуцированных поврежедений в лимфоцитах здоровых доноров (n = 103) проведено на основе Gn = 103) проведено на основе G = 103) проведено на основе GG2-теста. Использована
тест-система лимфоцитов периферической крови с последующим метафазным анализом. Результаты: на основе построения
калибровочных кривых “стадия-эффект”, “доза-эффект” разработана схема цитогенетического обследования условно
здоровых лиц. Анализ величин цитогенетических показателей, полученных при облучении в GG2-периоде клеточного цикла
в дозе 1,5 Гр, выявил их значительную вариабельность. Наибольшая вариабельность наблюдалась для аберраций хрома-
тидного типа (CV = 42,1%), в спектре которых преобладали одиночные хроматидные разрывы (CV = 37,5%). Статистичес-
кий анализ распределений индивидуальных значений полученных показателей позволил выявить 12% лиц с повышенной
радиационной чувствительностью хромосом. Выводы: цитогенетическая оценка индивидуальной радиочувствительности
на хромосомном уровне, основанная на G2-тесте, имеет перспективы применения при формировании групп повышенного
риска радиоиндуцированных злокачественных новообразований и их первичной профилактики среди населения.
Ключевые слова: радиочувствительность, GG2-тест, радиоиндуцированные новообразования.
Copyright © Experimental Oncology, 2007
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| id | nasplib_isofts_kiev_ua-123456789-138555 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T18:05:35Z |
| publishDate | 2007 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Dyomina, E.A. Ryabchenko, N.M. 2018-06-19T09:27:26Z 2018-06-19T09:27:26Z 2007 Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk / E.A. Dyomina, N.M. Ryabchenko // Experimental Oncology. — 2007. — Т. 29, № 3. — С. 217–220. — Бібліогр.: 19 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138555 Aim: Evaluation of chromosomal radiosensitivity of healthy individuals and determination those with the increased susceptibility to radiogenic cancer. Methods: Cytogenetic examination of radiation induced injuries in lymphocytes of healthy individuals (n = 103) was carried out on the basis of G2-assay. Test system of peripheral blood lymphocytes with metaphase analysis was used. Results: On the basis of the obtained “stage-effect” and “dose-effect” calibrating curves the scheme of cytogenetic examinations of healthy individuals was developed. Analysis of cytogenetic parameters induced by G2 irradiation at 1.5 Gy dose revealed their high interindividual variability. The highest differences were registered for chromatid type aberrations (CV = 42.1%) with the chromatid break predominance in the spectrum (CV = 37.5%). Statistical analysis of the distributions of the obtained individual cytogenetic parameters indicated 12% individuals with increased chromosomal radiosensitivity. Conclusions: Cytogenetic evaluation of individual chromosomal radiosensitivity based on G2-assay has its perspectives in the formation of groups with increased risk of radiogenic cancer developing and its primary prophylactics among healthy population. Цель: оценка радиочувствительности здоровых лиц на уровне хромосом лимфоцитов и определение индивидуумов с
 повышенным риском радиоиндуцированных новообразований. Методы: цитогенетическое исследование радиационно
 индуцированных поврежедений в лимфоцитах здоровых доноров (n = 103) проведено на основе G = 103) проведено на основе G2
 -теста. Использована
 тест-система лимфоцитов периферической крови с последующим метафазным анализом. Результаты: на основе построения
 калибровочных кривых “стадия-эффект”, “доза-эффект” разработана схема цитогенетического обследования условно
 здоровых лиц. Анализ величин цитогенетических показателей, полученных при облучении в G2
 -периоде клеточного цикла
 в дозе 1,5 Гр, выявил их значительную вариабельность. Наибольшая вариабельность наблюдалась для аберраций хроматидного
 типа (CV = 42,1%), в спектре которых преобладали одиночные хроматидные разрывы (CV = 37,5%). Статистический
 анализ распределений индивидуальных значений полученных показателей позволил выявить 12% лиц с повышенной
 радиационной чувствительностью хромосом. Выводы: цитогенетическая оценка индивидуальной радиочувствительности
 на хромосомном уровне, основанная на G2
 -тесте, имеет перспективы применения при формировании групп повышенного
 риска радиоиндуцированных злокачественных новообразований и их первичной профилактики среди населения. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk Повышенная индивидуальная радиочувствительность хромосом лимфоцитов человека как показатель риска злокачественных новообразований Article published earlier |
| spellingShingle | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk Dyomina, E.A. Ryabchenko, N.M. Original contributions |
| title | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk |
| title_alt | Повышенная индивидуальная радиочувствительность хромосом лимфоцитов человека как показатель риска злокачественных новообразований |
| title_full | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk |
| title_fullStr | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk |
| title_full_unstemmed | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk |
| title_short | Increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk |
| title_sort | increased individual chromosomal radiosensitivity of human lymphocytes as a parameter of cancer risk |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138555 |
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