Experimental study of the efficacy of combined use of cancer vaccine and interferon

Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subti...

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Veröffentlicht in:Experimental Oncology
Datum:2007
Hauptverfasser: Potebnya, G.P., Kudryavets, Yu.Y., Lisovenko, G.S., Cheremshenko, N.L., Voeykova, I.M., Trokhimenko, N.V., Symchich, T.V., Evstrateyva, L.M.
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Veröffentlicht: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2007
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Zitieren:Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id nasplib_isofts_kiev_ua-123456789-138575
record_format dspace
spelling Potebnya, G.P.
Kudryavets, Yu.Y.
Lisovenko, G.S.
Cheremshenko, N.L.
Voeykova, I.M.
Trokhimenko, N.V.
Symchich, T.V.
Evstrateyva, L.M.
2018-06-19T09:50:46Z
2018-06-19T09:50:46Z
2007
Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ.
1812-9269
https://nasplib.isofts.kiev.ua/handle/123456789/138575
Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subtilis B-7025, and preparation of murine IFN-alpha were used. Therapeutic effect was evaluated by measurement of tumor volume and analysis of average life span (ALS) of treated animals. Immunologic study included determination of antitumor cytotoxicity of T-lymphocytes (CTL) and natural killer (NK) cells by radiometric method, functional activity of peritoneal macrophages (MP) — by colorimetric test with nitroazole blue, and evaluation of titers of tumor necrosis factor (TNF) and interleukins-1 and -2 (IL-1, 2). Results: It has been shown that the use of IFN preparation significantly elevated efficacy of vaccine therapy of solid form of LLC: duration of latent period of tumor growth elevated by 25%, ALS — by 28%, index of tumor growth inhibition — by 35–40%. Upon combined use of CV and IFN, significant activation of the cells — effectors of nonspecific immune defense (MP), and specific one (CTL) was observed. Conclusion: The obtained results evidence on perspectiveness of the development of combined schemes of administration of CV and IFN for elevation of the efficacy of vaccine therapy.
Цель: исследовать в эксперименте эффективность комбинированной схемы введения противоопухолевой вакцины (ПВ) и интерферона (ИФН). Материалы и методы: карциному легкого Льюис (КЛЛ) трансплантировали мышам-самцам C57Bl. Для лечения использовали ПВ, приготовленную из клеток КЛЛ с помощью цитотоксических лектинов B. subtilis B-7025, и препарат мышиного ИФН. Терапевтический эффект оценивали путем измерения объема солидной опухоли и анализа средней продолжительности жизни опытных животных. Иммунологическое исследование включало определение противоопухолевой цитотоксичности Т-лимфоцитов (ЦТЛ) и природных киллерных клеток (ПКК) радиометрическим методом; функциональной активности перитонеальных макрофагов (Мф) в колориметрическом НСТ-тесте; определение титров фактора некроза опухоли (ФНО), интерлейкинов-1 и -2. Результаты: показано, что использование препарата ИФН существенно повышает эффективность вакцинотерапии солидной формы модельной КЛЛ: на 25% повышается продолжительность латентного периода, на 28% — средняя продолжительность жизни мышей, на 35–40% — индекс торможения опухолевого роста. При комбинированном применении ПВ и ИФН отмечают существенную активацию клеток-эффекторов как неспецифической (Мф), так и специфической (ЦТЛ) иммунной защиты. Выводы: полученные результаты свидетельствуют о перспективности разработки комбинированных схем введения ПВ с ИФН, позволяющих повысить эффективность вакцинотерапии.
en
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
Experimental Oncology
Original contributions
Experimental study of the efficacy of combined use of cancer vaccine and interferon
Экспериментальное исследование эффективности комбинированного использования противоопухолевой вакцины и интерферона
Article
published earlier
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
title Experimental study of the efficacy of combined use of cancer vaccine and interferon
spellingShingle Experimental study of the efficacy of combined use of cancer vaccine and interferon
Potebnya, G.P.
Kudryavets, Yu.Y.
Lisovenko, G.S.
Cheremshenko, N.L.
Voeykova, I.M.
Trokhimenko, N.V.
Symchich, T.V.
Evstrateyva, L.M.
Original contributions
title_short Experimental study of the efficacy of combined use of cancer vaccine and interferon
title_full Experimental study of the efficacy of combined use of cancer vaccine and interferon
title_fullStr Experimental study of the efficacy of combined use of cancer vaccine and interferon
title_full_unstemmed Experimental study of the efficacy of combined use of cancer vaccine and interferon
title_sort experimental study of the efficacy of combined use of cancer vaccine and interferon
author Potebnya, G.P.
Kudryavets, Yu.Y.
Lisovenko, G.S.
Cheremshenko, N.L.
Voeykova, I.M.
Trokhimenko, N.V.
Symchich, T.V.
Evstrateyva, L.M.
author_facet Potebnya, G.P.
Kudryavets, Yu.Y.
Lisovenko, G.S.
Cheremshenko, N.L.
Voeykova, I.M.
Trokhimenko, N.V.
Symchich, T.V.
Evstrateyva, L.M.
topic Original contributions
topic_facet Original contributions
publishDate 2007
language English
container_title Experimental Oncology
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
format Article
title_alt Экспериментальное исследование эффективности комбинированного использования противоопухолевой вакцины и интерферона
description Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subtilis B-7025, and preparation of murine IFN-alpha were used. Therapeutic effect was evaluated by measurement of tumor volume and analysis of average life span (ALS) of treated animals. Immunologic study included determination of antitumor cytotoxicity of T-lymphocytes (CTL) and natural killer (NK) cells by radiometric method, functional activity of peritoneal macrophages (MP) — by colorimetric test with nitroazole blue, and evaluation of titers of tumor necrosis factor (TNF) and interleukins-1 and -2 (IL-1, 2). Results: It has been shown that the use of IFN preparation significantly elevated efficacy of vaccine therapy of solid form of LLC: duration of latent period of tumor growth elevated by 25%, ALS — by 28%, index of tumor growth inhibition — by 35–40%. Upon combined use of CV and IFN, significant activation of the cells — effectors of nonspecific immune defense (MP), and specific one (CTL) was observed. Conclusion: The obtained results evidence on perspectiveness of the development of combined schemes of administration of CV and IFN for elevation of the efficacy of vaccine therapy. Цель: исследовать в эксперименте эффективность комбинированной схемы введения противоопухолевой вакцины (ПВ) и интерферона (ИФН). Материалы и методы: карциному легкого Льюис (КЛЛ) трансплантировали мышам-самцам C57Bl. Для лечения использовали ПВ, приготовленную из клеток КЛЛ с помощью цитотоксических лектинов B. subtilis B-7025, и препарат мышиного ИФН. Терапевтический эффект оценивали путем измерения объема солидной опухоли и анализа средней продолжительности жизни опытных животных. Иммунологическое исследование включало определение противоопухолевой цитотоксичности Т-лимфоцитов (ЦТЛ) и природных киллерных клеток (ПКК) радиометрическим методом; функциональной активности перитонеальных макрофагов (Мф) в колориметрическом НСТ-тесте; определение титров фактора некроза опухоли (ФНО), интерлейкинов-1 и -2. Результаты: показано, что использование препарата ИФН существенно повышает эффективность вакцинотерапии солидной формы модельной КЛЛ: на 25% повышается продолжительность латентного периода, на 28% — средняя продолжительность жизни мышей, на 35–40% — индекс торможения опухолевого роста. При комбинированном применении ПВ и ИФН отмечают существенную активацию клеток-эффекторов как неспецифической (Мф), так и специфической (ЦТЛ) иммунной защиты. Выводы: полученные результаты свидетельствуют о перспективности разработки комбинированных схем введения ПВ с ИФН, позволяющих повысить эффективность вакцинотерапии.
issn 1812-9269
url https://nasplib.isofts.kiev.ua/handle/123456789/138575
citation_txt Experimental study of the efficacy of combined use of cancer vaccine and interferon / G.P. Potebnya, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva // Experimental Oncology. — 2007. — Т. 29, № 2. — С. 102–105. — Бібліогр.: 23 назв. — англ.
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fulltext 102 Experimental Oncology 29, 102–105, 2007 (June) To improve the technology of cancer biotherapy, it is important to understand functions and mechanism of ac- tion of cytokines that are involved in attraction of immuno- competent cells to immune reactions and elevate ability of T-lymphocytes to eliminate malignant cells. That’s why biological modifiers of immune response are often used in technology of preparation of cancer vaccines (CV) or are used as their components. In the most simple variant, cytokines are administered together with antigen and stimulate immune response against it [1–4]. Interferon (IFN) is one of the key modulators of im- mune response influencing the processes of antigen recognition, differentiation, and functional activity of immunocompetent cells. The most interesting aspects of studies are effects of IFN related to elevation of tumor immunogenecity and its altered sensitivity to cytotoxic action of T-lymphocytes [5]. The role of IFN in combination with other biological agents or chemopreparations became a subject of intense studies. The research of effects of IFN upon combined use with active specific immunization is of special interest [1, 6, 7]. IFNs, including IFN-α2, -β, -γ, are potent inducers of expression of genes of major histocompatibility complex. Exactly this property, apart from indirect immunomodulating action is one of the main reasons for use of IFN in immunotherapy [8]. The facts on high anticancer acitivity of IFN in the case of metastatic melanoma and the increasing number of reports about positive influence of this cytokine on elongation of relapse-free period in the patients after tumor resection are evidencing the expediency of combined use of IFN with CV [6, 9, 10]. The mecha- nisms of synergistic action of vaccine and IFN possibly will differ dependent on composition of the vaccine and the immune reactions that provide an effect of immunization. In numerous reports it has been shown that IFN-α possesses the highest anticancer action [5, 6, 8]. The results of experimental and clinical studies are providing the grounds for the use of IFN-α as an element of optimization of combined therapy directed on the decrease of metastasis risk and improvement of quality of life of oncological patients [10, 11]. The aim of present research was to evaluate the possibilities of the use of immunomodulating properties of IFN for elevation of the efficacy of cancer vaccine prepared from autologous tumor cells with the use of cytotoxic lectin (CL) from B. subtilis B-7025 [12, 13]. MATERIALS AND METHODS The experiments were carried out on male С57Вl 2.5 months old mice bred in the vivarium of R.E. Ka- vetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine (Kyiv, Ukraine) with the use of experimental model of Lewis lung carci- noma (LLC). Tumor cells were transplanted in the thigh muscle (by 5.0 х 105 vital LLC cells per animal). The animals were treated by therapeutic scheme as follow: 1st group received CV, 2nd – preparation of mu- rine IFN, 3rd — CV + IFN by combined scheme. In this series of experiments, the group of untreated animals (4th group, transplantation control) and intact animals (5th group) served as the controls. All experiments with animals were approved by local ethic committee. CV was prepared from grounded LLC tissue samples treated with cytotoxic lectins of В. subtilis B-7025 (1 g of tumor tissue per 10 ml CL solution (0.5 mg/ml)) and stored at –18 ˚С [12]. Preparation of CL was isolated from culture medium of В. subtilis B-7025 by the method described earlier [14]. CV was EXPERIMENTAL STUDY OF THE EFFICACY OF COMBINED USE OF CANCER VACCINE AND INTERFERON G.P. Potebnya*, Yu.Y. Kudryavets, G.S. Lisovenko, N.L. Cheremshenko, I.M. Voeykova, N.V. Trokhimenko, T.V. Symchich, L.M. Evstrateyva R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine Aim: To study in in vivo model the efficacy of combined scheme of administration of cancer vaccine (CV) and interferon (IFN). Materials and Methods: Lewis lung carcinoma (LLC) was transplanted to male C57Bl mice. For treatment, CV prepared from LLC cells with the use of cytotoxic lectins of B. subtilis B-7025, and preparation of murine IFN-alpha were used. Therapeutic effect was evaluated by measurement of tumor volume and analysis of average life span (ALS) of treated animals. Immunologic study included determination of antitumor cytotoxicity of T-lymphocytes (CTL) and natural killer (NK) cells by radiometric method, functional activity of peritoneal macrophages (MP) — by colorimetric test with nitroazole blue, and evaluation of titers of tumor necrosis factor (TNF) and interleukins-1 and -2 (IL-1, 2). Results: It has been shown that the use of IFN preparation significantly elevated efficacy of vaccine therapy of solid form of LLC: duration of latent period of tumor growth elevated by 25%, ALS — by 28%, index of tumor growth inhibition — by 35–40%. Upon combined use of CV and IFN, significant activation of the cells — effectors of nonspecific immune defense (MP), and specific one (CTL) was observed. Conclusion: The obtained results evidence on perspectiveness of the development of combined schemes of administration of CV and IFN for elevation of the efficacy of vaccine therapy. Key Words: cancer vaccine, murine interferon, Lewis lung carcinoma, immunologic indexes. Received: March 28, 2007. *Correspondence: E-mail: iris@onconet.kiev.ua Abbreviations used: CTL — cytotoxic lymphocytes; CV — cancer vaccine; IFN — interferon; IL-1 — interleukin-1; LI — labeling in- dex; LLC — Lewis lung carcinoma; MP — peritoneal macrophages; NK — natural killer cells; TNF — tumor necrosis factor. Exp Oncol 2007 29, 2, 102–105 Experimental Oncology 29, 102–105, 2007 (June) 10329, 102–105, 2007 (June) 103June) 103) 103 103 injected subcutaneously (s. c.) by 0.3 ml at the days 1, 4, 8, 11, 15 after LLC transplantation. Preparation of IFN-alpha was prepared according to [15]. IFN solution was prepared ex tempore and ad- ministered 5 times intraperitoneally (i.p.) by 1000 units in 0.5 ml of physiologic solution 24 h prior to CV ad- ministration. Such scheme for IFN administration was chosen of the base of results of studies on combined use of CV with other immunomodulating preparations [16, 17]. Efficacy of treatment was evaluated by tumor growth inhibition index and average life span (ALS) of experimental animals. Immunologic research included determination of cytotoxic activity of T-lymphocytes (CTL) and natural killer (NK) cells in cytotoxic tests in vitro [18], cyto- chemical activity of peritoneal macrophages (MP) using test with nitroazole blue (NBT) [19], the level of production of tumor necrosis factor (TNF) by MP in vitro using TNF-sensitive L-929 cells [20], the levels of production of IL-1 by adherent fraction of spleen lymphocytes, and IL-2 — by nonadherent one using standard radiometric methods [21, 22]. These studies were carried out at the days 28 and 45 after tumor cells transplantation. Statistical analysis was carried out using Student’s t-criterium; the values p < 0.05 were considered sig- nificant [23]. RESULTS AND DISCUSSION The study of LLC growth evidenced on significant increase of the duration of latent period and pronounced inhibition of tumor growth upon combined use of CV and murine IFN compared with these indexes in the groups of transplantation control or upon separate use of CV and IFN (Fig. 1). Analogous peculiarities have been registered dur- ing analysis of ALS of experimental animals (Table 1). In the group of LLC-bearing mice that received CV at monoregi- men (group 1), insignificant increase of the duration of latent period has been observed as well as tumor growth inhibition by 24–45%, that lead to significant increase of ALS. Administration of CV with IFN (group 3) led to signifi- cant increase of its efficacy: the duration of latent period elevated by 25%, whilst tumor growth inhibition increased by 35–40%. ALS indexes in groups 1 and 3 were 53.71 ± 4.0 and 65.0 ± 2.9 days respectively (p < 0.05). It should be stressed that upon separate use murine IFN also was effective compared to control indexes: the duration of latent period elevated by 39%, ALS — by 38% (54.8 ± 3.75 and 39.8 ± 1.8 days respectively, p < 0.05). So, the obtained results have shown that aadministration of IFN to LLC-bearing mice at monoregimen or in combination with vaccine elevates vaccine’s efficacy. The study of immunologic indexes allowed to anali- ze their changes in treated animals (Table 2). In control group of LLC-bearing mice at the day 28 the cytotoxic activity of NK cells and CTL has been restored at relatively high level (labeling index (LI) was +322.6% and +196.0%, respectively compared to intact control). These indexes as well as MP activity (LI = +79.4%) were significantly higher than these of intact animals (p < 0.05). Fig. 1. Duration of latent period (a) and tumor growth inhibition of experimental animals *p < 0.05 compared to control of transplantation. Table 1. Average life span (ALS) and duration of latent period in mice bearing Lewis lung carcinoma Group Treat- ment Number of ani- mals ALS, days Latent period, days Х ± m t LI, % Х ± m t LI, % I CV 14 53.7 ± 4.0 2.8* 35.0 11.1 ± 1.3 1.4 13.5 ІІ IFN 15 54.8 ± 3.8 3.1* 37.7 12.5 ± 1.1 2.5* 28.3 ІІI CV + IFN 11 65.0 ± 3.9 5.8* 63.3 13.5 ± 1.6 2.7* 38.2 ІV Control 10 39.8 ± 1.8 – – 9.0 ± 0.8 – – *p < 0.05 compared to control of transplantation. Table 2. Activity of effectors of cellular antitumor immunity in mice bearing Lewis lung carcinoma (day 28 of tumor growth) Group Treatment CI, % Activity of MP in NBT-testNK CTL I CV 43.2 ± 2.3*, ** 7.8 ± 4.5*** 0.176*** ІІ IFN 9.4 ± 4.7*** 28.0 ± 4.3* 0.243 IІІ CV + IFN 10.6 ± 5.2*** 34.6 ± 2.4* 0.600*, *** ІV Control of transplantation 35.5 ± 2.6* 24.5 ± 13.8* 0.393* V Intact control 6.4 ± 2.0 2.0 ± 1.2 0.219 *р < 0.05 compared to intact control; **0.05 < р < 0.1 compared to control of transplantation; ***р < 0.05 compared to control of transplantation. Administration of CV to mice resulted in the marked NK cells activation (43.2 ± 2.3 versus 35.5 ± 2.6% for group of transplantation control, p < 0.05); at the same time the indexes of activities of CTL and MP remained at the level of intact control and were significantly lower than these of transplantation control group. Upon the use of IFN, the situation was different — the preparation acted mainly via activation of CTL. Com- bined treatment with both agents (CV + IFN) along by increased specific response was accompanied with elevation of MP activity (compared to transplantation control group, LI was +41% and +52,7%, respectively, p < 0.05), whilst NK cells cytotoxicity remained at the level of that of intact animals. It is known that along with effectors of specific and natural resistance, cytokines (IL-1, IL-2, TNF, IFN) play 104 Experimental Oncology 29, 102–105, 2007 (June) an important role in anticancer defense of the body. As biologic modifiers of immune response, they are often used in technology of preparation of cancer vac- cines or are used as their components. That’s why one may suppose that positive effects of vaccine therapy combined with IFN could be in part explained by its influence on the level of production at least some of mentioned cytokines, in particular, TNF, IL-1, IL-2. At the day 28 of tumor growth, production of TNF in animals from transplantation control group was de- creased compared with intact control: this is evidenced by relatively low titer of TNF (LI = –51.9%, р < 0.05) (Table 3). It should be noted that at this time point in experimental groups (groups І–ІІІ) production of TNF remained at the level of intact animals only in mice that received IFN (the titer of TNF activity in this group was 2.55 ± 0.25 log2 and was twice higher than such indexes of transplantation control group as well as other experimental groups. At this period no alterations in IL-1 activity have been observed in all studied groups. Level of IL-2 production in the supernatants of cul- tured lymphocytes isolated at day 28 from the animals of transplantation control group was significantly lower than that of intact animals (LI = –56.8%, р < 0.05). At this period in mice from groups І and ІІ the titer of IL-2І and ІІ the titer of IL-2 and ІІ the titer of IL-2ІІ the titer of IL-2 the titer of IL-2 activity was practically equal to that of tumor-bearing control mice (LI was –35% and –16%, respectively, compared to transplantation control group). The titers of IL-2 activity in mice that received IFN + CV were close to these of intact control (LI = –36% compared to in- tact control, and +46.8% compared to transplantation control group) (Table 4). It’s interesting that exactly in this group the highest percent of animals (25%) that didn’t develop tumors has been registered. Table 4. Production of IL-2* by Т-lymphocytes from spleen of С57Bl mice after transplantation of Lewis lung carcinoma cells Group of animals Activity of CTL, CI, % Terms of observation (days after tumor cell transplantation) 28 45 CV 7.8 ± 4.52 < 1 log2 1, 2 – CV + IFN 34.6 ± 2.41 2.26 ± 0.011, 2 2.68 ± 0.071, 3 IFN 28.0 ± 4.31 1.29 ± 0.011, 2 – Control of transplantation 24.5 ± 3.81 1.54 ± 0.11 – Intact control 2.0 ± 1.2 3.57 ± 0.08 *Production of IL-2 is expressed in titres of activity, log2; 1p < 0.05 compared to intact control; 2p < 0.05 compared to control of transplantation; 3p < 0.05 compared to previous observation point. That’s why it looks reasonable to analyze the state of effectors of anticancer defense (CTL, NK, MP) (Fig. 2) in these animals. Immunologic research has been carried out at day 45 after LLC transplantation (at this term all animals from transplantation control group died, ALS = 39.8 ± 1.8 days). The results have shown significant activation of effector cells of specific and unspecific defense (p < 0.05). At this term of observa- tion the highest CTL activity has been registered in mice that received IFN separately or in combination with CV (LI was +993.0% and +615.6% respectively, p < 0.05, compared to intact animals). The indexes of NK and MP activity in mice of experimental groups were similar and were significantly higher than control ones. Fig. 2. Activity of effectors of cell immunity in mice with unde- veloped tumors (day 45 after LLC transplantation), that received CV + IFN by combined scheme: a — cytotoxicity of NK and CTL, b — activity of MP in NBT-test. In conclusion, the presented data allow suppose that the use of IFN at monoregimen or in combination with CV favors restoraton of the functional reserve of all effectors of antitumor defense of the body, and that combined administration of CV and IFN could be considered as a promising tool. Further exploration of synergistic action of these preparations could be useful for the development of effective schemes of biotherapy of oncologic diseases. REFERENCES 1. Dillman RO, Wiemann M, Nayak SK, De Leon C, Hood K, DePriest C. Interferon-gamma or granulocyte-macro- phage colony-stimulating factor administered as adjuvants with Table 3. Production of TNF and IL-1 in vitro by adherent splenocytes of mice bearing Lewis lung carcinoma that received CV + IFN by combined scheme Cyto- kine Group Titer of activity, log2 Cytotoxicity index, % 1 : 2 1 : 4 1 : 8 1 : 16 1 : 32 1 : 64 TNF CV 1.13 ± 0.29 127.5 ± 20.2 23.6 ± 8.8 0 0 0 0 IFN 2.55 ± 0.25** 279.2 ± 33.7 133.1 ± 20.8 57.3 ± 14.0 9.5 ± 2.43 0 0 CV + IFN 1.50 ± 0.19 102.2 ± 17.9 16.2 ± 12.5 0 0 0 0 Control of transplantation 1.25 ± 0.31 75.3 ± 15.4 49.4 ± 23.3 0 0 0 0 Intact control 2.6 ± 0.4 119.1 ± 20.4 107.9 ± 7.7 62.9 ± 6.8 6.7 ± 2.1 0 0 IL-1 CV 1.3 ± 0.3 2.6 ± 0.1 1.8 ± 0.4 1.3 ± 0.2 1.1 ± 0.04 0,8 ± 0,04 0,8 ± 0,2 IFN 2.0 ± 0.0 2.8 ± 0.4 2.6 ± 0.3 1.6 ± 0.2 0.9 ± 0.2 0,6 ± 0,1 0,5 ± 0,1 CV + IFN 1.0 ± 0.6 2.3 ± 0.3 1.9 ± 0.3 1.2 ± 0.2 1.1 ± 0.1 1,0 ± 0,1 1,1 ± 0,03 Control of transplantation 2.0 ± 0.0 3.2 ± 0.3 2.4 ± 0.4 1.6 ± 0.3 1.2 ± 0.1 1,6 ± 0,02 1,1 ± 0,04 Intact control 2.4 ± 0.4 2.5 ± 0.04 2.3 ± 0.07 1.9 ± 0.05 1.6 ± 0.3 0,8 ± 0,1 1,1 ± 0,06 *p < 0.05 compared to intact control; **p < 0.05 compared to control of transplantation; ***p<0,05 p < 0.05 compared to previous observation point. 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Материалы и методы: карциному легкого Льюис (КЛЛ) трансплантировали мышам-самцам C57Bl. Для лечения использовали ПВ, приготовленную из клеток КЛЛ с помощью цитотоксических лектинов B. subtilis B-7025, и препарат мышиного ИФН. Терапевтический эффект оценивали путем измерения объема солидной опухоли и анализа средней продолжительности жизни опытных животных. Иммунологическое исследование включало определение противоопухолевой цитотоксичности Т-лимфоцитов (ЦТЛ) и природных киллерных клеток (ПКК) радиометрическим методом; функциональной активности перитонеальных макрофагов (Мф) в колориметрическом НСТ-тесте; определение титров фактора некроза опухоли (ФНО), интерлейкинов-1 и -2. Результаты: показано, что использование препарата ИФН существенно повышает эффективность вакцинотерапии солидной формы модельной КЛЛ: на 25% повышается продолжительность латентного периода, на 28% — средняя продолжительность жизни мышей, на 35–40% — индекс торможения опухолевого роста. При комбинированном применении ПВ и ИФН отмечают существенную активацию клеток-эффекторов как неспецифической (Мф), так и специфической (ЦТЛ) иммунной защиты. Выводы: полученные результаты свидетельствуют о перспективности разработки комбинированных схем введения ПВ с ИФН, позволяющих повысить эффективность вакцинотерапии. Ключевые слова: противоопухолевая вакцина, интерферон, карцинома легкого Льюис, иммунные показатели. Copyright © Experimental Oncology, 2007