TGF-β1 expression by glioma C6 cells in vitro
The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of...
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| Veröffentlicht in: | Experimental Oncology |
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| Datum: | 2017 |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2017
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| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/138583 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| _version_ | 1862710957169442816 |
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| author | Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. |
| author_facet | Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. |
| citation_txt | TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ. |
| collection | DSpace DC |
| container_title | Experimental Oncology |
| description | The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS.
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| first_indexed | 2025-12-07T17:27:46Z |
| format | Article |
| fulltext | |
| id | nasplib_isofts_kiev_ua-123456789-138583 |
| institution | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| issn | 1812-9269 |
| language | English |
| last_indexed | 2025-12-07T17:27:46Z |
| publishDate | 2017 |
| publisher | Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| record_format | dspace |
| spelling | Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. 2018-06-19T10:00:39Z 2018-06-19T10:00:39Z 2017 TGF-β1 expression by glioma C6 cells in vitro / L.D. Liubich, L.M. Kovalevska, M.I. Lisyany, V.M. Semenova, T.A. Malysheva, L.P. Stayno, V.V. Vaslovych // Experimental Oncology. — 2017 — Т. 39, № 4. — С. 258–263. — Бібліогр.: 40 назв. — англ. 1812-9269 https://nasplib.isofts.kiev.ua/handle/123456789/138583 The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS. en Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України Experimental Oncology Original contributions TGF-β1 expression by glioma C6 cells in vitro Article published earlier |
| spellingShingle | TGF-β1 expression by glioma C6 cells in vitro Liubich, L.D. Kovalevska, L.M. Lisyany, M.I. Semenova, V.M. Malysheva, T.A. Stayno, L.P. Vaslovych, V.V. Original contributions |
| title | TGF-β1 expression by glioma C6 cells in vitro |
| title_full | TGF-β1 expression by glioma C6 cells in vitro |
| title_fullStr | TGF-β1 expression by glioma C6 cells in vitro |
| title_full_unstemmed | TGF-β1 expression by glioma C6 cells in vitro |
| title_short | TGF-β1 expression by glioma C6 cells in vitro |
| title_sort | tgf-β1 expression by glioma c6 cells in vitro |
| topic | Original contributions |
| topic_facet | Original contributions |
| url | https://nasplib.isofts.kiev.ua/handle/123456789/138583 |
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